TWo METHODS FOR THE DEMONSTRATION OF NORADRENALINE-CONTAINING ADRENAL MEDULLARY CELLS

Hopse, Väinö K.; Mäkinen, Eero

doi:10.1177/14.5.434

Cited by 16 publications

(7 citation statements)

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“…Hopsu and Mäkinen’s staining is one of the classical methods. This method is based on the reduction of NBT salt at alkaline pH essentially as in the demonstration of sulfhydryl groups (Hopsu and Mäkinen 1966). To determine the cell type of hematoxylin-stained chromaffin cells, we used this method.…”

Section: Resultsmentioning

confidence: 99%

“…We followed Hopsu and Mäkinen’s staining method (Hopsu and Mäkinen 1966) except for the buffer system. In brief, paraffin sections were deparaffinized in xylene and ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…Despite extensive research, however, the differences between the two types of chromaffin cells remain unclear partly because of a difficulty in differential demonstration of E cells and NE cells (especially in the mouse medulla). Indeed, many histochemical methods do exist to distinguish the two cell types (Coupland and Hopwood 1966; Honore 1971; Hopsu and Mäkinen 1966). However, these methods, which involve special histologic procedures, have certain practical drawbacks that impair their general usefulness (Honore 1971).…”

Section: Introductionmentioning

confidence: 99%

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A simple and sensitive method for the demonstration of norepinephrine-storing adrenomedullary chromaffin cells

Khan

Lee

Kamitani

2012

Histochem Cell Biol

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Section: Resultsmentioning

confidence: 99%

“…We followed Hopsu and Mäkinen’s staining method (Hopsu and Mäkinen 1966) except for the buffer system. In brief, paraffin sections were deparaffinized in xylene and ethanol.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A simple and sensitive method for the demonstration of norepinephrine-storing adrenomedullary chromaffin cells

Khan

Lee

Kamitani

2012

Histochem Cell Biol

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“…In addition, three tests quoted by Barka and Anderson (1963) were used: diazotized saffranin for 5- HT (Lillie et al 1953), the astra-blue method for acid mucopolysaccharides (Pioch 1958), and the potassium iodate method for adrenalin (Hillarp and Hokfelt 1955). The presence of noradrenalin was determined by the tetrazolium and ferric-ferricyanide reactions (Hopsu and Makinen 1966) after glutaraldehyde fixation. The same fixation preceded tests for indoles (Solcia et al 1966).…”

Section: Methodsmentioning

confidence: 99%

HISTOCHEMICAL ANALYSIS OF THE NEUROSECRETORY CELLS IN THE ADULT BRAIN OFTRIPHAENA PRONUBA(LEPIDOPTERA: NOCTUIDAE)

Hinks

1971

Can Entomol

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Four major categories of neurosecretory cells (A, B, C, D) were distinguished in the brain of Triphaena pronuba L. by staining sections with paraldehyde fuchsin and counterstains. These comprised nine types of cells, seven of which were recognizable for analysis of their secretory products. Histochemical tests indicate that the lepidopteron brain has a multiplicity of neurosecretory cells, each type producing chemically different secretions.

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“…PC18, a second APN inhibitor, inhibits APN 2150-fold (Ki = 8 nM) more strongly than APA and 125-fold more strongly than aminopeptidase B (EC 3.4.11.6). The latter is another monozinc aminopeptidase that preferentially cleaves N-terminal basic residues [59,60]. These compounds were used in vivo [32,61] to study the metabolism of brain angiotensins (Fig.…”

Section: Identification Of the In Vivo Metabolic Pathways Of Angii Anmentioning

confidence: 99%

Aminopeptidase A inhibitors as centrally acting antihypertensive agents

et al. 2008

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Among the main bioactive peptides of the brain renin-angiotensin system, angiotensin (Ang) II and AngIII exhibit the same affinity for the type 1 and type 2 Ang receptors. Both peptides, injected intracerebroventricularly, cause similar increase in blood pressure (BP). Because AngII is converted in vivo to AngIII, the identity of the true effector is unknown. This review summarized recent insights into the predominant role of brain AngIII in the central control of BP underlining the fact that brain aminopeptidase A (APA), the enzyme forming central AngIII, could constitute a putative central therapeutic target for the treatment of hypertension. This led to the development of potent, systematically active APA inhibitors, such as RB150, as a prototype of a new class of centrally acting antihypertensive agents for the treatment of certain forms of hypertension.

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TWo METHODS FOR THE DEMONSTRATION OF NORADRENALINE-CONTAINING ADRENAL MEDULLARY CELLS

Cited by 16 publications

References 0 publications

A simple and sensitive method for the demonstration of norepinephrine-storing adrenomedullary chromaffin cells

A simple and sensitive method for the demonstration of norepinephrine-storing adrenomedullary chromaffin cells

HISTOCHEMICAL ANALYSIS OF THE NEUROSECRETORY CELLS IN THE ADULT BRAIN OFTRIPHAENA PRONUBA(LEPIDOPTERA: NOCTUIDAE)

Aminopeptidase A inhibitors as centrally acting antihypertensive agents

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