Two muscle-specific LIM proteins in Drosophila.

Stronach, Beth; Siegrist, Sarah E.; Beckerle, Mary C.

doi:10.1083/jcb.134.5.1179

Cited by 70 publications

(97 citation statements)

References 47 publications

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“…This gene was suggested to have a role in the maintenance of cytosolic Ca 2+ levels, which may become raised as part of the stress response. Transcripts for a gene with 94% identity at the amino acid level to D. melanogaster LIM protein (Stronach et al 1996) were found in the goldenrod gall moth E. scudderiana after exposure to low temperatures (Bilgen at al 2001). Muscle LIM proteins are essential for myogenesis and promote differentiation of striated muscle (Arber et al 1994).…”

Section: Mitochondrial Degradationmentioning

confidence: 99%

How insects survive the cold: molecular mechanisms—a review

2008

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Insects vary considerably in their ability to survive low temperatures. The tractability of these organisms to experimentation has lead to considerable physiology-based work investigating both the variability between species and the actual mechanisms themselves. This has highlighted a range of strategies including freeze tolerance, freeze avoidance, protective dehydration and rapid cold hardening, which are often associated with the production of specific chemicals such as antifreezes and polyol cryoprotectants. But we are still far from identifying the critical elements behind overwintering success and how some species can regularly survive temperatures below -20°C. Molecular biology is the most recent tool to be added to the insect physiologist's armoury. With the public availability of the genome sequence of model insects such as Drosophila and the production of custom-made molecular resources, such as EST libraries and microarrays, we are now in a position to start dissecting the molecular mechanisms behind some of these well-characterised physiological responses. This review aims to provide a state of the art snapshot of the molecular work currently being conducted into insect cold tolerance and the very interesting preliminary results from such studies, which provide great promise for the future.

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Section: Mitochondrial Degradationmentioning

confidence: 99%

How insects survive the cold: molecular mechanisms—a review

2008

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“…In situ hybridization to embryos was performed as described (Stronach et al 1996). The dpp antisense riboprobe, corresponding to dpp coding sequence, was generated by use of the Boehringer Mannheim Genius RNA labeling kit according to the manufacturer.…”

Section: Staining and Imagingmentioning

confidence: 99%

Activation of the JNK pathway during dorsal closure in Drosophila requires the mixed lineage kinase, slipper

Stronach¹,

Perrimon²

2002

Genes Dev.

104

111

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The Jun kinase (JNK) pathway has been characterized for its role in stimulating AP-1 activity and for modulating the balance between cell growth and death during development, inflammation, and cancer. Six families of mammalian kinases acting at the level of JNKKK have emerged as upstream regulators of JNK activity (MLK, LZK, TAK, ASK, MEKK, and TPL); however, the specificity underlying which kinase is utilized for transducing a distinct signal is poorly understood. In Drosophila, JNK signaling plays a central role in dorsal closure, controlling cell fate and cell sheet morphogenesis during embryogenesis. Notably, in the fly genome, there are single homologs of each of the mammalian JNKKK families. Here, we identify mutations in one of those, a mixed lineage kinase, named slipper (slpr), and show that it is required for JNK activation during dorsal closure. Furthermore, our results show that other putative JNKKKs cannot compensate for the loss of slpr function and, thus, may regulate other JNK or MAPK-dependent processes.

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“…MLP in vertebrates also initiates a stretchregulated downstream response, a hypertrophic program that leads to an increase in the number of sarcomeres, likely through its ability to translocate from the Z-disk to the nucleus, where it associates with muscle-specific transcriptional activators (Arber et al, 1994;Knöll et al, 2002). In contrast, Drosophila Mlp(s) display some transient nuclear localization, but with no proven nuclear function, because an Mlp84B transgene carrying a nuclear export signal can fully rescue the pupal lethality of mlp84B mutants (Stronach et al, 1996;Clark et al, 2007).…”

Section: The Z-disk As a Focal Point For Force Propagationmentioning

confidence: 99%

“…1). The Drosophila MLP family is encoded by two genes, mlp60A and mlp84B, and their protein products are both detected at the periphery of Z-disks and at myotendinous junctions (Stronach et al, 1996;Clark et al, 2007). Interestingly, despite its early localization, Mlp84B depletion results in late muscle defects observed just before pupation.…”

Section: The Z-disk As a Focal Point For Force Propagationmentioning

confidence: 99%

Molecular mechanisms of mechanosensing in muscle development

Jani

Schöck

2009

Developmental Dynamics

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Mechanical forces are crucial to muscle development and function, but the mechanisms by which forces are sensed and transduced remain elusive. Evidence implicates the sarcolemmal lattice of integrin adhesion and the Z-disk components of the contractile machinery in such processes. These mechanosensory devices report changes in force to other cellular compartments by self-remodeling. Here we explore how their structural and functional properties integrate to regulate muscle development and maintenance.

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Two muscle-specific LIM proteins in Drosophila.

Abstract: Abstract. The LIM domain defines a zinc-binding motif found in a growing number of eukaryotic proteins that regulate cell growth and differentiation during development

Cited by 70 publications

References 47 publications

How insects survive the cold: molecular mechanisms—a review

How insects survive the cold: molecular mechanisms—a review

Activation of the JNK pathway during dorsal closure in Drosophila requires the mixed lineage kinase, slipper

Molecular mechanisms of mechanosensing in muscle development

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