Two new Clitocella species from North China revealed by phylogenetic analyses and morphological characters

Abstract

Two new species of Clitocella are proposed based on morphological and phylogenetic investigations. Clitocella borealichinensissp. nov. is closely related to C. orientalis but distinguished from the latter by its slightly smaller basidiospores and hyphae of pileipellis with pale brown to brown intracellular or parietal pigment. Clitocella coloratasp. nov. is closely related to C. popinalis and C. mundula in macromorphology but is differentiated from C. popinalis by its slightly smaller basidiospores and the difference in genetic profile, and from C. mundula by its relatively colorful pileus (white to yellowish white, grayish white to grayish brown, pink white). Phylogenetic analyses based on sequence data from five different loci (ITS, nrLSU, tef1, rpb2 and atp6) support the taxonomic position of the two new species in the genus Clitocella. The illustrations and descriptions for the new taxa are provided.

Overview of the European species of the genus Clitocella (Entolomataceae, Agaricales) with notes on extralimital taxa

“…The .phy file was loaded into the RAxML v. 8 program ( Stamatakis 2014 ) using the standard search algorithm with 1000 bootstrap cycles according to the GTR + GAMMA model. Clitopilus prunulus was used as outgroup following Baroni et al (2020) and Mao et al (2022) . The trees in .tre format were read with the software SEAVIEW v. 4 ( Gouy et al 2010 ) and saved in a vector format for printing.…”

“…Notes — It is a widespread saprotrophic genus known from Europe, Asia, North, South and Central America in forested or in grassy areas and also on dunes ( Baroni 1981 , Contu 2002 , Noordeloos 1988a , 2012a , Moreau et al 2008 , Kluting et al 2014 , Kour et al 2016 , Silva-Filho et al 2018 , Baroni et al 2020 , Jian et al 2020 , Mao et al 2022 ). Clitocella species are usually terrestrial but C. termitophila grows on arboreal nests of neotropical termites ( Baroni et al 2020 ).…”

“…Phylogenetically it is sister to the genus Clitopilus , typified with Agaricus prunulus . The highly supported clade Clitocella + Clitopilus is sister to Clitopilopsis , typified with Agaricus hirneolus ( Co-David et al 2009 , Kluting et al 2014 , Morgado et al 2016 , Vizzini et al 2016 , Sánchez-García & Matheny 2017 , Boccardo & Dovana 2019 , Baroni et al 2020 , Sánchez-García et al 2020 , Mao et al 2022 ). Most species of Clitopilus show the same clitocyboid habit as Clitocella but are distinguished by basidiospores which are ornamented with pustules arranged in longitudinal ridges ( Noordeloos 1988b , 2012b ).…”

“…popinalis ( Kour et al 2016 ) is C. colorata ( Fig. 1 and phylogenetic analysis in Mao et al 2022 ). Rhodocybe popinalis var.…”

“…The aim of the present paper is to provide a detailed overview of the species of Clitocella present in Europe based on morphological and molecular phylogenetic multigene analyses (nrITS, nr28S, RPB2 , EF-1α and ATP6 ) of collections from different areas, comparing them also with the Clitocella species recently described from Asia and Central and South America ( Silva-Filho et al 2018 , Baroni et al 2020 , Jian et al 2020 , Mao et al 2022 ). Clitocella was established to accommodate the rhodocyboid species with crowded, narrow, long-decurrent lamellae, absence of clamp connections, and thin-walled, obscurely pustulate binucleate basidiospores ( Kühner & Lamoure 1971 , Kluting et al 2014 ).…”