Two new disposable bioreactors for plant cell culture: The wave and undertow bioreactor and the slug bubble bioreactor

Terrier, Bénédicte; Courtois, Didier; Hénault, Nicolas; Cuvier, Arnaud; Bastin, Maryse; Aknin, Aziz; Dubreuil, Julien; Pétiard, Vincent

doi:10.1002/bit.21187

Cited by 102 publications

(50 citation statements)

References 44 publications

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“…Mass transfer has been reported to be higher in the center of the bubble column as compared to that near the walls due to the presence of a larger number of bubbles in the center. Higher gas holdup (indicating the volume of gas phase occupied by gas bubbles in the liquid phase) is also reported to be crucial for better design of bubble column reactor (Bouaifi et al 2001;Luo et al 1999;Terrier et al 2007).…”

Section: Discussionmentioning

confidence: 94%

Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors

Patra

Srivastava

2015

Plant Cell Rep

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Alternative biotechnological protocol for large-scale artemisinin production was established. It featured enhanced growth and artemisinin production by cultivation of hairy roots in nutrient mist bioreactor (NMB) coupled with novel cultivation strategies. Artemisinin is used for the treatment of cerebral malaria. Presently, its main source is from seasonal plant Artemisia annua. This study featured investigation of growth and artemisinin production by A. annua hairy roots (induced by Agrobacterium rhizogenes-mediated genetic transformation of explants) in three bioreactor configurations-bubble column reactor, NMB and modified NMB particularly to establish their suitability for commercial production. It was observed that cultivation of hairy roots in a non-stirred bubble column reactor exhibited a biomass accumulation of 5.68 g/l only while batch cultivation in a custom-made NMB exhibited a higher biomass concentration of 8.52 g/l but relatively lower artemisinin accumulation of 0.22 mg/g was observed in this reactor. A mixture of submerged liquid-phase growth (for 5 days) followed by gas-phase cultivation in nutrient mist reactor operation strategy (for next 15 days) was adopted for hairy root cultivation in this investigation. Reasonably, high (23.02 g/l) final dry weight along with the artemisinin accumulation (1.12 mg/g, equivalent to 25.78 mg/l artemisinin) was obtained in this bioreactor, which is the highest reported artemisinin yield in the gas-phase NMB cultivation.

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Section: Discussionmentioning

confidence: 94%

Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors

Patra

Srivastava

2015

Plant Cell Rep

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“…(Goyal and Ramawat 2008a , b ;Sharma et al 2009 ;He et al 2011 ), Psoralea sp. (Shinde et al 2009a , b ), and Glycine max (Federici et al 2003 ;Terrier et al 2007 ). Calycosin, formononetin, and pseudobaptigenin are also present in the more widespread legume Trifolium pratense (Kokotkiewicz et al 2013 ).…”

Section: Isofl Avone Productionmentioning

confidence: 95%

Improving Secondary Metabolite Production in Tissue Cultures

Kumar

2015

Plant Biology and Biotechnology

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Plant cell and tissue culture has been suggested as an alternative means for year-round production of secondary metabolites with an added potential of increasing yields by culture selection and manipulation, genetic transformation, hairy root cultures, and use of bioreactors for mass production. Secondary metabolite pathways and genes involved in those pathways have been identifi ed, and regulation of transcription and transcription factors has been determined by studying functional genomics in conjunction with data-mining tools of bioinformatics. Besides this, advances in metabolic engineering enable researchers to confer new secondary metabolic pathways to crops by transferring three to fi ve, or more, heterologous genes taken from various other species. As an alternative, the metabolic pathways of useful secondary metabolites have been modifi ed to improve their productivity via genetic transformation. However, there is a need to understand metabolic pathways of secondary metabolism at the molecular level. Plant hairy roots offer a novel and sustainable tissue-based system that preserves multiple specialized cell types believed to be important in maintaining a better consistency in synthesis of bioactive secondary molecules. This paper will review stateof-the-art reports on improving production of secondary metabolites in tissue cultures in various plant species.

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“…5). The latest disposable bioreactor developments, including the Wave & Undertow bioreactor (WU bioreactor) and the Slug Bubble bioreactor (SB bioreactor), were successfully applied to grow tobacco and soya cells expressing isoflavones and monoclonal antibodies (anti-rabies) up to 100 L culture volume (Girard et al 2006;Terrier et al 2006). The WU bioreactor is based on the WIM principle like BioWave, while the SB bioreactor represents a bubble column that allows an easy increase of size by using multiple units.…”

Section: Suitable Bioreactors For Plant Cell Suspension Culturesmentioning

confidence: 99%

Plant Cell-Based Bioprocessing

Eibl

2009

Cell and Tissue Reaction Engineering

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Plant cell-based bioprocessing is the use of plant cell and tissue cultures for the production of biologically active substances (low molecular secondary metabolites and recombinant proteins). The most significant advantage of plant cell culture over the traditionally grown whole wild plant or engineered transgenic plant is the sterile production of metabolites under defined controlled conditions independent of climatic changes and soil conditions, which means that variations in product yield and quality can be better avoided. Furthermore, regulatory requirements such as the cGMP standards, which have to be adhered to in the early stages of pharmaceutical production, are more easily met.Moreover, plant cells are capable of performing complex posttranslational processing, which is a precondition for heterologous protein expression. When compared with mammalian cells, which currently dominate in the commercial protein manufacture, plant cell cultures as alternative expression systems guarantee safer processes because there is a lower risk of contamination by mammalian viruses, pathogens, and toxins. In addition to this considerable advantage, the process costs can also be substantially reduced. This is due to the fact that plant cell culture medium is very simple in composition and therefore relatively inexpensive.This chapter provides an overview of culture types, techniques, and suitable bioreactors used to produce secondary metabolites and recombinant proteins in plant cells. We describe plant cell culture basics, discuss key topics relevant to plant cell bioreactor engineering with application examples, and give an overview of approaches to improving productivity of plant cell-based processes.

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Two new disposable bioreactors for plant cell culture: The wave and undertow bioreactor and the slug bubble bioreactor

Cited by 102 publications

References 44 publications

Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors

Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors

Improving Secondary Metabolite Production in Tissue Cultures

Plant Cell-Based Bioprocessing

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