Two new Ophiostoma species lacking conidial states isolated from bark beetles and bark beetle-infested Abies species in Japan

Abstract

Two ophiostomatoid fungi without conidiophores and conidia were isolated from bark beetles and bark beetle-infested Abies spp. The teleomorph characters were similar to species of Ophiostoma, but the absence of the conidial stage hampered their exact generic assignment. Detailed morphological observations and molecular analysis clarified their generic affiliation. Consequently, they were placed in the genus Ophiostoma , but their morphology and DNA sequences did not coincide with any other species of Ophiostoma. Here, we propose these two species as Ophiostoma aoshimae sp. nov. and Ophiostoma rectangulosporium sp. nov. The former species is characterized by perithecial necks ornamented with projections and ellipsoid to oblong ascospores, and the latter species is characterized by perithecial necks ornamented with rectangular ascospores and white colonies.

Fungi associated with Ips acuminatus (Coleoptera: Curculionidae) in Ukraine with a special emphasis on pathogenicity of ophiostomatoid species

“…Moreover, in 2006 G. rectangulosporium was also described from Japan in association with the bark beetle infesting Abies spp. (Ohtaka et al, 2006) and in 2009 from China in association with Dendroctonus valens Le Conte (Coleoptera: Curculionidae) infesting Pinus tabuliformis Carr. (Lu et al, 2009).…”

Genetic characterization of Raffaelea quercivora isolates collected from areas of oak wilt in Japan

“…The genetic characterization of fungal ribosomal DNA (rDNA) has become commonly used for the identification and placement of the ambrosia fungi, Ambrosiella and Raffaelea, and other ophiostomatoid fungi (Cassar and Blackwell 1996;Jones and Blackwell 1998;Farrell et al 2001;Ohtaka et al 2006). The internal transcribed spacer (ITS) region of rDNA has been used for species discrimination because its basal sequences are highly variable between species but low within species (Bruns et al 1991;Lee and Taylor 1992;Nilsson et al 2008).…”

Multigene phylogenies of Ophiostomataceae associated with Monterey pine bark beetles in Spain reveal three new fungal species

“…All these species have hyalorhinocladiella-to pesotum-like anamorphs, except Gra. rectangulosporium, for which no anamorph has been observed (Ohtaka et al 2006).…”

“…microcarpum (Yamaoka et al 2004, dark brown to black conidiophores), Gra. rectangulosporium (Ohtaka et al 2006) and seven undescribed taxa, one of which is described here as Gra. crescericum.…”