Two new mutations in dnaJ suppress DNA damage hypersensitivity and capsule overproduction phenotypes of Δlon mutant of Escherichia coli by modulating the expression of clpYQ (hslUV) and rcsA genes

Kumaran, Nagarajan; Karthik, Maruthan; Kumar, Vikram; Tennyson, Jebasingh; Munavar, M. Hussain

doi:10.1016/j.gene.2019.144135

Cited by 4 publications

(1 citation statement)

References 42 publications

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“…33 The Zwf gene was overexpressed to promote accessibility NADPH and GTP for optimal 2′-FL synthesis. 34 The clpYQ gene encodes a protease that can also be used to inhibit RcsA and RcsB regulators, 35 and Lon acts in the same way.…”

Section: ■ Results and Disscusionmentioning

confidence: 99%

High-Yield Synthesis of 2′-Fucosyllactose from Glycerol and Glucose in Engineered Escherichia coli

Wang,

Zhang,

Gao

et al. 2023

J. Agric. Food Chem.

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2′-Fucosyllactose (2′-FL) is vital for the growth and development of newborns. In this study, we developed a synthesis pathway for 2′-FL in Escherichia coli BL21 (DE3). Then, we optimized the solubility of α-1,2-fucosyltransferase, thereby enhancing the production yield of 2′-FL. Based on this finding, we further enhanced the expression of guanosine inosine kinase Gsk and knocked out the isocitrate lyase regulator gene iclR. This strategy reduced the formation of byproduct acetate during the metabolic process and alleviated carbon source overflow effects in the strain, resulting in further improvement of the yield of 2′-FL. In a 3 L bioreactor, employing fed-batch fermentation with glycerol and glucose as substrates, the engineered strain BWLAI-RSZL exhibited impressive 2′-FL titers of 121.9 and 111.56 g/L, along with productivity levels of 1.57 and 1.31 g/L/h, respectively. The reported 2′-FL titers reached a groundbreaking level, irrespective of the carbon source employed (glycerol or glucose), highlighting the significant potential for large-scale industrial synthesis of 2′-FL.

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Section: ■ Results and Disscusionmentioning

confidence: 99%

High-Yield Synthesis of 2′-Fucosyllactose from Glycerol and Glucose in Engineered Escherichia coli

Wang,

Zhang,

Gao

et al. 2023

J. Agric. Food Chem.

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Effect of Clp protease from Corynebacterium glutamicum on heterologous protein expression

Liu

Meng

Wang

et al. 2022

Protein Expression and Purification

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Proteomic survey of the DNA damage response inCaulobacter crescentus

Tashjian

Zeinert

Eyles³

2023

Preprint

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The bacterial DNA damage response is a critical, coordinated response to DNA replication stress. The canonical bacterial DNA damage response, first characterized in Escherichia coli, is controlled by the global transcriptional regulator LexA and the recombinase RecA. While genome-wide studies have described how the DNA damage response is regulated at a transcriptional level, relatively little is known about post-transcriptional regulation of this response. Here, we perform a proteome-wide survey of the DNA damage response in Caulobacter crescentus. We find that not all changes in protein abundance during the response to DNA damage are predicted by changes in transcription. We validate one of these post-transcriptionally regulated candidates to show its importance to survival of DNA damage. To investigate post-translational control of the DNA damage response, we perform a similar survey in cells lacking the Lon protease. This reveals that induction of the DNA damage response at the protein level is dampened in these strains, consistent with their reduced tolerance to DNA damage. Finally, proteome-wide stability measurements following damage nominate candidate Lon substrates that suggest post-translational regulation of the DNA damage response. The DNA damage response helps bacteria to respond to and potentially survive DNA damage. The mutagenesis that is induced as part of this response plays a role in bacterial evolution and is essential to the development and spread of antibiotic resistance. Understanding how bacteria coordinate their response to DNA damage could help us to combat this growing threat to human health. While the transcriptional regulation of the bacterial DNA damage response has been characterized, this study is the first to our knowledge to compare changes in RNA and protein levels to identify potential targets of post-transcriptional regulation in response to DNA damage.

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Two new mutations in dnaJ suppress DNA damage hypersensitivity and capsule overproduction phenotypes of Δlon mutant of Escherichia coli by modulating the expression of clpYQ (hslUV) and rcsA genes

Cited by 4 publications

References 42 publications

High-Yield Synthesis of 2′-Fucosyllactose from Glycerol and Glucose in Engineered Escherichia coli

High-Yield Synthesis of 2′-Fucosyllactose from Glycerol and Glucose in Engineered Escherichia coli

Effect of Clp protease from Corynebacterium glutamicum on heterologous protein expression

Proteomic survey of the DNA damage response inCaulobacter crescentus

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