Two new Rahnella genomospecies that cannot be phenotypically differentiated from Rahnella aquatilis

Brenner, Don J.; Müller, H. E.; Steigerwalt, Arnold G.; Whitney, Anne M.; O'Hara, C M; Kämpfer, Peter

doi:10.1099/00207713-48-1-141

Cited by 51 publications

(39 citation statements)

References 28 publications

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“…Remarkably, in that study, several DGGE bands identified as Rahnella spp. were found (Table 2), showing the closest homology to genomospecies 2, identified previously by Brenner et al (6).…”

Section: Vol 72 2006supporting

confidence: 65%

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Changes in the Spoilage-Related Microbiota of Beef during Refrigerated Storage under Different Packaging Conditions

Ercolini

Russo

Torrieri

et al. 2006

Appl Environ Microbiol

392

244

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The microbial spoilage of beef was monitored during storage at 5°C under three different conditions of modified-atmosphere packaging (MAP): (i) air (MAP1), (ii) 60% O 2 and 40% CO 2 (MAP2), and (iii) 20% O 2 and 40% CO 2 (MAP3). Pseudomonas, Enterobacteriaceae, Brochothrix thermosphacta, and lactic acid bacteria were monitored by viable counts and PCR-denaturing gradient gel electrophoresis (DGGE) analysis during 14 days of storage. Moreover, headspace gas composition, weight loss, and beef color change were also determined at each sampling time. Overall, MAP2 was shown to have the best protective effect, keeping the microbial loads and color change to acceptable levels in the first 7 days of refrigerated storage. The microbial colonies from the plate counts of each microbial group were identified by PCR-DGGE of the variable V6-V8 region of the 16S rRNA gene. Thirteen different genera and at least 17 different species were identified after sequencing of DGGE fragments that showed a wide diversity of spoilage-related bacteria taking turns during beef storage in the function of the packaging conditions. The countable species for each spoilage-related microbial group were different according to packaging conditions and times of storage. In fact, the DGGE profiles displayed significant changes during time and depending on the initial atmosphere used. The spoilage occurred between 7 and 14 days of storage, and the microbial species found in the spoiled meat varied according to the packaging conditions. Rahnella aquatilis, Rahnella spp., Pseudomonas spp., and Carnobacterium divergens were identified as acting during beef storage in air (MAP1). Pseudomonas spp. and Lactobacillus sakei were found in beef stored under MAP conditions with high oxygen content (MAP2), while Rahnella spp. and L. sakei were the main species found during storage using MAP3. The identification of the spoilage-related microbiota by molecular methods can help in the effective establishment of storage conditions for fresh meat.

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Section: Vol 72 2006supporting

confidence: 65%

“…In addition, Rahnella aquatilis can be associated with human infections (52). Two other Rahnella genomospecies that are not phenotypically distinguishable from Rahnella aquatilis were characterized previously by Brenner et al (6). Remarkably, in that study, several DGGE bands identified as Rahnella spp.…”

Section: Vol 72 2006mentioning

confidence: 77%

Changes in the Spoilage-Related Microbiota of Beef during Refrigerated Storage under Different Packaging Conditions

Ercolini

Russo

Torrieri

et al. 2006

Appl Environ Microbiol

392

244

View full text Add to dashboard Cite

show abstract

“…As no phenotypic differentiation of these strains from other strains within biogroup 16 could be determined and only two strain exist in this group, at the present time it is proposed to designate a novel genomospecies [33] represented by strain NCTC 9529. This strain was originally isolated from water and deposited at the NCTC, London, UK, in 1954.…”

Section: Resultsmentioning

confidence: 99%

The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies 1

et al. 2007

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Background Enterobacter sakazakii is an opportunistic pathogen that can cause infections such as necrotizing enterocolitis, bacteraemia, meningitis and brain abscess/lesions. When the species was defined in 1980, 15 biogroups were described and it was suggested that these could represent multiple species. In this study the taxonomic relationship of strains described as E. sakazakii was further investigated. Results Strains identified as E. sakazakii were divided into separate groups on the basis of f-AFLP fingerprints, ribopatterns and full-length 16S rRNA gene sequences. DNA-DNA hybridizations revealed five genomospecies. The phenotypic profiles of the genomospecies were determined and biochemical markers identified. Conclusion This study clarifies the taxonomy of E. sakazakii and proposes a reclassification of these organisms.

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“…Two Rahnella genomospecies were described in 1998, but they are not readily distinguishable from R. aquatilis, and they have not yet been named (3). Reference strains of genomovar 1 are pyrase positive and are more susceptible to quinolones than those of genomovar 2, which are pyrase negative (19).…”

Section: Discussionmentioning

confidence: 99%

Rahnella aquatilis Bacteremia from a Suspected Urinary Source

Tash

2005

J Clin Microbiol

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A 76-year-old male with prostatic hyperplasia presented with acute pyelonephritis. Blood cultures yielded Rahnella aquatilis. Treatment with intravenous followed by oral levofloxacin resulted in cure. Important characteristics of this organism include its biochemical similarities to Enterobacter agglomerans, its apparent ability to cause bacteremia from a renal focus, and its response to quinolone therapy. CASE REPORTA 76-year-old male presented to the emergency room complaining of nausea, vomiting, and "shaking." His medical history was significant for benign prostatic hypertrophy and a urinary tract infection 6 months previously. Physical examination revealed a fever of 103.7°F, a blood pressure of 109/57 mm Hg, a pulse of 112 beats/min, and a respiratory rate of 18 breaths/min; the physical examination was otherwise unremarkable. The complete blood count was significant for 18,700 white blood cells and 94% granulocytes with a left shift. Urinalysis revealed "many" bacteria and red and white blood cells and was leukocyte esterase positive. One dose (500 mg) of intravenous levofloxacin and fluids were administered for pyelonephritis. The patient was discharged on 500 mg of oral levofloxacin daily for 7 days. Two days later, blood cultures from separate sites grew gram-negative bacilli, which were subsequently identified as Rahnella aquatilis using the API system (bioMérieux). The isolate was sensitive to amoxicillinclavulanic acid,

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Two new Rahnella genomospecies that cannot be phenotypically differentiated from Rahnella aquatilis

Cited by 51 publications

References 28 publications

Changes in the Spoilage-Related Microbiota of Beef during Refrigerated Storage under Different Packaging Conditions

Changes in the Spoilage-Related Microbiota of Beef during Refrigerated Storage under Different Packaging Conditions

Rahnella aquatilis Bacteremia from a Suspected Urinary Source

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