A new caddisflies species Holocentropus totuttotam sp. nov. and Nyctiophylax totaktoetak sp. nov. (Polycentropodidae) are described and illustrated from Rovno amber (Priabonian). Holocentropus atratus (Pictet, 1856) and Holocentropus vetustus (Germar, 1813) are recorded for Rovno amber for the first time. Now the family Polycentropodidae is represented by 23 named species among 42 named species of caddisflies known from Rovno amber. A list of fossil Trichoptera known from the Rovno amber is given.