Two new species of Hypoxylon (Hypoxylaceae) from China based on morphological and DNA sequence data analyses

Abstract

Two new species, Hypoxylon larissae and Hypoxylon jianfengense, are described and illustrated on the basis of a combination of morphological and molecular characters from China. Hypoxylon larissae was collected from southwest China (Yunnan province), while H. jianfengense was collected from southern China (Hainan province). Hypoxylon larissae is characterized by effused-pulvinate stromata, Orange (7) and Luteous (12) pigments extracted in 10% KOH and light brown to dark brown ascospores with straight germ slit. It is phylogenetically and morphologically closely related to H. fenderi. Hypoxylon jianfengense is distinguished by tubular to long tubular perithecia, Dark Green (21), Isabelline (65) and Dull Green (70) pigments extracted in 10% KOH and conspicuous coil-like perispore ornamentation. Hypoxylon jianfengense clustered with H. invadens and H. trugodes in a well-supported clade. Phylogenetic analyses of multi-gene sequences (ITS-LSU-RPB2-TUB2) support the recognition of the taxa as distinct.

Taxonomic and phylogenetic contributions to Diatrypaceae from southeastern Tibet in China

“…The studied specimens were macromorphologically observed with the aid of a VHX-600E microscope of Keyence Corporation (Osaka, Japan) up to ×200. The microscopic procedure followed Song et al (2022) . Specimen sections were mounted in water, 10% potassium hydroxide (KOH), and Melzer’s reagent (1.5 g potassium iodide, 0.5 g crystalline iodine, and 22 g chloral hydrate dissolved in 20 ml distilled water), and then microscopic examinations were carried out with an Olympus IX73 inverted fluorescence microscope (Tokyo, Japan) at magnifications up to × 1,000.…”

Three New Species of Hypoxylon (Xylariales, Ascomycota) on a Multigene Phylogeny from Medog in Southwest China

“…The stroma and perithecia were observed, photographed and measured with a VHX-600E 3D microscope from the Keyence Corporation (Osaka, Japan). Fresh material was respectively immersed in water, 10% KOH, and Melzer’s reagent to observe micromorphological structures as determined by Ma et al and Song et al [ 20 , 21 ]. The observations, micrographs, and measurements of asci and ascospores were performed by using an Olympus IX73 inverted fluorescence microscope (Olympus, Tokyo, Japan) and the CellSens Dimensions Software (Olympus, Tokyo, Japan).…”

“…Fresh tissue of stroma was used for DNA extraction and sequence generation following the suggestions by Ma et al and Song et al [ 20 , 21 ]. Sequences of four DNA loci—ITS (internal transcribed spacer regions), nrLSU (nuclear large subunit ribosomal DNA), RPB2 (RNA polymerase II second largest subunit), and β-tubulin (beta-tubulin) were selected for multi-gene phylogenetic analyses [ 2 , 25 ].…”

“…The alignment, trimming, and concatenation of sequences followed Song et al [ 21 ]. The multi-gene phylogenetic analyses were performed by using two methods of maximum likelihood (ML) and Bayesian analyses (BA) based on ITS-LSU-RPB2-β-tubulin datasets and ITS-β-tubulin datasets.…”

“…In this genus, more than 200 species with 1189 epithets included in the Index Fungorum have been reported so far [ 4 , 15 , 16 ]. Despite species of Hypoxylon being widely distributed throughout Asia, only 57 species were reported in China currently [ 17 , 18 , 19 , 20 , 21 ].…”