Holocene diatoms of the Colorado River Delta, Argentina. A paleoenvironmental approach from the study of "small fragilarioids". Diatom assemblages of a sedimentary succession in the deltaic plain of the Colorado River (Argentina) were studied in order to reconstruct the paleoenvironmental conditions of the delta in response to Holocene sea-level changes. A 191 cm depth core was extracted by vibracoring from Las Isletas (LI), located 22 km from the coast (39°43'5.7"S, 62°20'49.7"W). A cluster analysis -CONISS of 35 subsamples defined three diatom zones (DZ). The sedimentary succession is dominated by small fragilarioids, being Punctastriata glubokoensis Williams, Chudaev & Gololobova the most abundant species; the accompanying flora included fresh-brackish and allochthonous marine-coastal taxa. At the base, subzone DZIA (191-163 cm, 7,315±64 14 C yr BP) included sandy lithological units and presented abundant marine-coastal species. The overlying subzone DZIB (163-96 cm) has low abundance of allochthonous marine species. The uppermost zone, DZII (96 cm from the top) integrate the silt-sandy lithological units, and it was deposited ca. 2,218±29 14 C yr BP. DZII lacked marine species. The LI sequence represents the infilling of a river-estuarine channel with tidal influence ca. 7,300 14 C yr BP that gradually evolved into a fresh-brackish river channel, similar to the current river, ca. 2,200 14 C yr BP. During the Middle Holocene transgression, the greatest marine influence would have transported coastal marine species upstream to the LI site; the increase of fluvial influence associated with the progradation of the delta lobe would have favored the ubiquity and persistence of the small fragilarioids in the fluvial-delta assemblages of the Colorado River during the last ca. 7,000 14 C yr BP.