Two New Sulfated Furostanol Saponins from Tribulus terrestris

Abstract

The known furostanol saponins methylprotodioscin and protodioscin and two new sulfated saponins, sodium salt of 26-O-β-glucopyranosyl-22α-methoxy-(25R)-furost-5-ene-3β,26-diol- 3-O-α-rhamnopyranosyl-(1→2)-β-4-O-sulfo-glucopyranoside (methylprototribestin) and sodium salt of 26-O-β-glucopyranosyl-22α-hydroxy-(25R)-furost-5-ene-3β,26-diol-3-O-α-rhamnopyranosyl-( 1→2)-β-4-O-sulfo-glucopyranoside (prototribestin) have been isolated from the aerial parts of Tribulus terrestris L. growing in Bulgaria. The structures of the new compounds were elucidated on the basis of 1D and 2D (DQF-COSY, TOCSY, HSQC-TOCSY, HSQC, HMBC, ROESY) NMR data, ESI mass spectra and chemical transformation.

Complex Investigations ofTribulus TerrestrisL. for Sustainable use by Pharmaceutical Industry

“…of Phytochemistry at the Bulgarian Academy of Sciences (32,33,35) have shown that the two main components of T. terrestris, grown in Bulgaria, are protodioscin (1) and prototribestin (3) and not protodioscin (1) and protogracilin (2) as previously reported by other authors (56,57) (Fig. 1).…”

“…terrestris from Southhungary, samples natively distributed in Spain and turkey and the results were compared with the samples natively distributed in Bulgaria. the study of the content of the steroidal saponins protodioscin, prototribestin, dioscin and rutin in various samples of T. terrestris were profiled and quantified using HPLC method described in our previous works (12,13,32,33).…”

A Study onTribulus TerrestrisL. Anatomy and Ecological Adaptation

“…it has been known as a valuable medicinal plant for a long time. the plant is used directly as a herb or as a main component for production of a number of medicines and food supplements (1,7,8). the high and consistent use of Tribulus terrestris by the pharmaceutical industry has led to a production demand and fostered the need for its semi-cultivation.…”

Intraspecific Variability of Biologically Active Compounds of Different Populations ofTribulus TerrestrisL. (Zygophyllaceae) in South Bulgaria

“…it is also included in many dietary supplements claimed to have a biostimulating activity (2). Several studies have shown that steroidal saponins are among the compounds responsible for the biological activities of T. terrestris (1,3,4,5). …”