Two new triterpenoid saponins obtained by microbial hydrolysis withAlternaria alternataAS 3.6872

Abstract

Compound 1, a triterpenoid saponin from Ardisia gigantifolia Stapf showing potential anti-tumour activity, was hydrolysed into two deglycosyl derivatives (2 and 3) by Alternaria alternata AS 3.6872. Both these derivatives are new compounds. Their structures were elucidated on the basis of 1D, 2D NMR, HR-ESI-MS and optical rotation spectral data. Compounds 1-3 were evaluated for their cytotoxicity against human hepatocellular carcinoma and normal liver cells by Cell Counting Kit 8 colorimetric assay.

AG36 Inhibits Human Breast Cancer Cells Proliferation by Promotion of Apoptosis In vitro and In vivo

“…AG36 (purity: >99%) was the biotransformation product of triterpenoid saponin AG4 from A. gigantifolia stapf. as previously described ( Mu et al, 2015 ). The Dulbecco’s phosphate buffered saline (DPBS), protease inhibitor cocktail, gelatin, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA).…”

“…Among them, cyclamiretin A 3β-O-{α- L -rhamnopyranosyl-(1→3)-[β- D -xylopyranosyl-(1→2)]-β- D -glucopyranosyl-(1→4)-[β- D -gluco-pyranosyl-(1→2)]-α- L -arabinopyranoside} (AG4) had prom-inent cytotoxicity against MCF-7 cells ( Zheng et al, 2013 ). In order to discover new anticancer lead compounds, AG4 was biotransformated by Alternaria alternata AS 3.6872 to obtain AG36 ( Mu et al, 2015 ). The structure of AG36 is similar with that of AG4, but with four-sugar units at C-3 ( Figure 1 ), and AG36 showed better cytotoxicity than AG4 against human breast cancer MCF-7 cells.…”

A new Secondary metabolites of the crinoid (Comanthina schlegeli) associated fungus Alternaria brassicae 93

“…However, there are few reports about crinoid associated fungi and their metabolites. In addition, Alternaria species play a significant role in new compounds discovery Mu et al 2015;. Accordingly, we took the crinoid-associated fungus Alternaria brassicae 93 as a new resource for novel compounds.…”

Triterpenoid Saponins from <i>Ardisia gigantifolia</i> and Mechanism on Inhibiting Proliferation of MDA-MB-231 Cells

“…This type of triterpenoid saponins showed cytotoxicity against a variety of human cancer cell lines through cell cycle arresting, apoptosis inducing and microtubule disassembling, which demonstrate the potential anti-cancer properties of the saponins. [4][5][6][7][8] We have isolated thirteen triterpenoid saponins (1,(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) from the rhizomes of A. gigantifolia STAPF. and obtained four new triterpenoid saponins (2-5) by biotransformation of compound 1.…”

“…and obtained four new triterpenoid saponins (2-5) by biotransformation of compound 1. [9][10][11][12][13] Some of them have prominent cytotoxicity against breast cancer cell lines. 14) In order to identify the active moieties for development of novel drugs against breast cancers, we reported the structure-activity relationship (SAR) of compounds 1-17 and MCF-7.…”