Two Nonrecombining Sympatric Forms of the Human Malaria Parasite<i>Plasmodium ovale</i>Occur Globally

Sutherland, Colin J.; Tanomsing, Naowarat; Nolder, Debbie; Oguike, Mary C.; Jennison, Charlie; Pukrittayakamee, Sasithon; Dolecek, Christiane; Hien, Tran Tinh; Rosário, Virgílio Estólio do; Arez, Ana Paula; Pinto, João; Michon, P; Escalante, Ananías A.; Nosten, François; Burke, Martin; Lee, Rogan; Blaze, Marie; Otto, Thomas D.; Barnwell, John W.; Pain, Arnab; Williams, John E.; White, Nicholas J.; Day, Nicholas P. J.; Snounou, Georges; Lockhart, Peter J.; Chiodini, Peter L.; Imwong, Mallika; Polley, Spencer D.

doi:10.1086/652240

Cited by 354 publications

(401 citation statements)

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“…The species of Plasmodium that infect humans are: P. falciparum, P. vivax, P. malariae, P. knowlesi, P. ovale curtisi and P. ovale wallikeri. 5 Plasmodium falciparum is the most prevalent and lethal. 1 Plasmodium falciparum accounts for about 98% of malaria cases in Nigeria while P. malariae occurs as mixed infection with P. falciparum.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of malaria parasites among blood donors in Kaduna, Nigeria

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Prevalence of malaria parasites among blood donors in Kaduna, Nigeria

et al. 2016

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“…P. ovale had the lowest detection rate of the four species (70%), though it was assayed the least frequently, in only 10 tests. The three false-negative results occurred in labs that reported other false negatives; therefore, it is unclear if the errors resulted from laboratory procedures or from an inability to detect the dimorphic P. ovale (16).…”

Section: 23mentioning

confidence: 99%

A Quality Control Program within a Clinical Trial Consortium for PCR Protocols To Detect Plasmodium Species

et al. 2014

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v Malaria parasite infections that are only detectable by molecular methods are highly prevalent and represent a potential transmission reservoir. The methods used to detect these infections are not standardized, and their operating characteristics are often unknown. We designed a proficiency panel of Plasmodium spp. in order to compare the accuracy of parasite detection of molecular protocols used by labs in a clinical trial consortium. Ten dried blood spots (DBSs) were assembled that contained P. falciparum, P. vivax, P. malariae, and P. ovale; DBSs contained either a single species or a species mixed with P. falciparum. DBS panels were tested in 9 participating laboratories in a masked fashion. Of 90 tests, 68 (75.6%) were correct; there were 20 false-negative results and 2 false positives. The detection rate was 77.8% (49/63) for P. falciparum, 91.7% (11/12) for P. vivax, 83.3% (10/12) for P. malariae, and 70% (7/10) for P. ovale. Most false-negative P. falciparum results were from samples with an estimated <5 parasites per l of blood. Between labs, accuracy ranged from 100% to 50%. In one lab, the inability to detect species in mixed-species infections prompted a redesign and improvement of the assay. Most PCR-based protocols were able to detect P. falciparum and P. vivax at higher densities, but these assays may not reliably detect parasites in samples with low P. falciparum densities. Accordingly, formal quality assurance for PCR should be employed whenever this method is used for diagnosis or surveillance. Such efforts will be important if PCR is to be widely employed to assist malaria elimination efforts.

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“…Five species of malaria can be transmitted to humans. Severe disease is largely caused by P. falciparum, while the diseases caused by P. vivax, P. ovale [Sutherland et al, 2010] and P. malariae are generally milder and rarely fatal. P. knowlesi is a zoonosis that causes malaria in macaques but sometimes can infect humans [Fong et al, 1971;Singh et al, 2004].…”

Section: Plasmodium Sppmentioning

confidence: 99%