Two novel heteropolymer‐forming proteins maintain the multicellular shape of the cyanobacterium <i>Anabaena</i> sp. PCC 7120

Springstein, Benjamin L.; Nürnberg, Dennis J.; Woehle, Christian; Weißenbach, Julia; Theune, Marius L.; Helbig, Andreas O.; Maldener, Iris; Stucken, Karina

doi:10.1111/febs.15630

Cited by 7 publications

(7 citation statements)

References 99 publications

(157 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Homologues to the heteropolymer-forming IFs ZicK and ZacK, recently discovered in the cyanobacterium Anabaena spp. [98], are also present in the Ca . T. nelsonii genomes.…”

Section: Discussionmentioning

confidence: 99%

Outside-in: intracellular vesicles in giant sulfur bacteria contain peptidoglycan

Flood

Leprich

Hunter

et al. 2022

Preprint

View full text Add to dashboard Cite

Until recently, the cellular envelopes of bacteria were regarded as static and rigid relative to those of eukaryotes. While investigating peptidoglycan synthesis in populations of giant sulfur bacteria, Candidatus Thiomargarita spp., we observed internal vesicle-like features (VLFs). VLFs, as imaged following the active incorporation of D-amino acids, appear to begin as invaginations and delaminations of the cellular envelope. Staining with wheat germ agglutinin confirmed the presence of peptidoglycan in VLFs, while polymyxin B revealed that the outer membrane is present in some VLFs. Transmission electron microscopy revealed a complex network of interconnected VLFs. Genomes of Ca . Thiomargarita nelsonii lack a canonical divisome, while possessing homologs to genes such as actin, membrane scaffolding proteins, and dynamins that are associated with phagocytosis in eukaryotes. The physiological role of VLFs remains unclear, but the presence of sulfur globules in some suggests compartmentalization of metabolism and energy production. This is the first report of peptidoglycan and outer membrane bound intracellular vesicles within prokaryotic cells. These findings transform the canonical view of the inflexible bacterial cell envelope and further narrow the divide between prokaryotes and eukaryotes.

show abstract

“…Homologues to the heteropolymer-forming IFs ZicK and ZacK, recently discovered in the cyanobacterium Anabaena spp. [98], are also present in the Ca . T. nelsonii genomes.…”

Section: Discussionmentioning

confidence: 99%

Outside-in: intracellular vesicles in giant sulfur bacteria contain peptidoglycan

Flood

Leprich

Hunter

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The employed in vitro polymerization assay using an unspecific NHS-fluorescein dye proved to be a valuable tool to conveniently detect polymerizing proteins in vitro (for a list of polymerizing cyanobacterial proteins see Figure 4) and could facilitate the identification of other filamentous proteins. We are currently working on the additional characterization of several Anabaena CCRPs and initial results indicate that some of those CCRPs, including ZicK and ZacK, could be involved in the stabilization of the linear trichome phenotype in Anabaena, extending the known impact of CCRPs from cell shape to trichome shape [211]. Noteworthy, ZicK and ZacK were also observed to be strictly interdependent to form heteropolymers in vitro and in vivo, describing a novel trait of bacterial CCRPs [211].…”

Section: Coiled-coil-rich Proteins In Cyanobacteriamentioning

confidence: 97%

“…We are currently working on the additional characterization of several Anabaena CCRPs and initial results indicate that some of those CCRPs, including ZicK and ZacK, could be involved in the stabilization of the linear trichome phenotype in Anabaena, extending the known impact of CCRPs from cell shape to trichome shape [211]. Noteworthy, ZicK and ZacK were also observed to be strictly interdependent to form heteropolymers in vitro and in vivo, describing a novel trait of bacterial CCRPs [211].…”

Section: Coiled-coil-rich Proteins In Cyanobacteriamentioning

confidence: 97%

“…Depiction of coiled-coil-rich-regions (purple rectangles) in (A) the human vimentin, (B) previously described bacterial CCRPs, and (C) recently identified cyanobacterial CCRPs. Coiled-coil-rich regions were predicted using the COILS algorithm [212] or were obtained from [9,197,204,211]. Orange rectangles indicate TPR repeats that are also identified as coiled-coils by the COILS algorithm.…”

Section: Coiled-coil-rich Proteins In Cyanobacteriamentioning

confidence: 99%

“…Orange rectangles indicate TPR repeats that are also identified as coiled-coils by the COILS algorithm. Vimentin [188]; TlpA [192]; Crescentin [9]; FilP [197], Syc2039, HmpF Syc , Slr7083, HmpF Syn , Fm7001, All4981 [204]; ZicK, ZacK [211].…”

Section: Coiled-coil-rich Proteins In Cyanobacteriamentioning

confidence: 99%

See 2 more Smart Citations

Structural Determinants and Their Role in Cyanobacterial Morphogenesis

Springstein

Nürnberg

Weiss

et al. 2020

Life

Self Cite

View full text Add to dashboard Cite

Cells have to erect and sustain an organized and dynamically adaptable structure for an efficient mode of operation that allows drastic morphological changes during cell growth and cell division. These manifold tasks are complied by the so-called cytoskeleton and its associated proteins. In bacteria, FtsZ and MreB, the bacterial homologs to tubulin and actin, respectively, as well as coiled-coil-rich proteins of intermediate filament (IF)-like function to fulfil these tasks. Despite generally being characterized as Gram-negative, cyanobacteria have a remarkably thick peptidoglycan layer and possess Gram-positive-specific cell division proteins such as SepF and DivIVA-like proteins, besides Gram-negative and cyanobacterial-specific cell division proteins like MinE, SepI, ZipN (Ftn2) and ZipS (Ftn6). The diversity of cellular morphologies and cell growth strategies in cyanobacteria could therefore be the result of additional unidentified structural determinants such as cytoskeletal proteins. In this article, we review the current advances in the understanding of the cyanobacterial cell shape, cell division and cell growth.

show abstract

Identification of structural and regulatory cell-shape determinants in Haloferax volcanii

Schiller,

Hong,

Kouassi

et al. 2024

Nat Commun

View full text Add to dashboard Cite

Archaea play indispensable roles in global biogeochemical cycles, yet many crucial cellular processes, including cell-shape determination, are poorly understood. Haloferax volcanii, a model haloarchaeon, forms rods and disks, depending on growth conditions. Here, we used a combination of iterative proteomics, genetics, and live-cell imaging to identify mutants that only form rods or disks. We compared the proteomes of the mutants with wild-type cells across growth phases, thereby distinguishing between protein abundance changes specific to cell shape and those related to growth phases. The results identified a diverse set of proteins, including predicted transporters, transducers, signaling components, and transcriptional regulators, as important for cell-shape determination. Through phenotypic characterization of deletion strains, we established that rod-determining factor A (RdfA) and disk-determining factor A (DdfA) are required for the formation of rods and disks, respectively. We also identified structural proteins, including an actin homolog that plays a role in disk-shape morphogenesis, which we named volactin. Using live-cell imaging, we determined volactin’s cellular localization and showed its dynamic polymerization and depolymerization. Our results provide insights into archaeal cell-shape determination, with possible implications for understanding the evolution of cell morphology regulation across domains.

show abstract

Two novel heteropolymer‐forming proteins maintain the multicellular shape of the cyanobacterium Anabaena sp. PCC 7120

Abstract: Supinfo: 1 Supinfo Two novel heteropolymer-forming proteins maintain the multicellular shape of the cyanobacterium Anabaena sp. PCC 7120

Cited by 7 publications

References 99 publications

Outside-in: intracellular vesicles in giant sulfur bacteria contain peptidoglycan

Outside-in: intracellular vesicles in giant sulfur bacteria contain peptidoglycan

Structural Determinants and Their Role in Cyanobacterial Morphogenesis

Identification of structural and regulatory cell-shape determinants in Haloferax volcanii

Contact Info

Product

Resources

About