Two novel HMW MAP2 variants with four microtubule‐binding repeats and different projection domains

Langkopf, Anja; Guilleminot, Jocelyne; Nunez, Jacques

doi:10.1016/0014-5793(94)01128-1

Cited by 14 publications

(5 citation statements)

References 29 publications

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“…This isoform has been described so far only in a neuronal cell line (Langkopf et al 1994) and only very low levels are present in the adult brain (Doll et al 1993;Kindler and Garner 1994).…”

Section: Discussionmentioning

confidence: 94%

Expression of microtubule-associated proteins MAP2 and tau in cultured rat brain oligodendrocytes

Müller

Heinrich

Heck

et al. 1997

Cell and Tissue Research

114

106

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Oligodendrocytes in culture are characterized by large membranous sheets containing an elaborate network of microtubules. Microtubule-associated proteins (MAPs) participate in microtubule stability and the regulation of the cellular architecture. We have investigated the expression of two major groups of MAPs, MAP2 and tau, in cultured rat brain oligodendrocytes. Alternatively spliced isoforms of mRNAs encoding MAP2 and tau were assessed by means of reverse transcription and polymerase chain reaction using a newly designed set of MAP2- and tau-specific primers. The data were compared with data obtained with cultures of rat brain astrocytes and rat cerebral neurons, and adult rat brain. The results show that oligodendrocytes, similarly to neurons, express mainly MAP2c transcripts containing three microtubule-binding repeats. They also contain small amounts of MAP2b mRNA. Six low molecular weight tau isoforms, namely tau 1-6, have been described in the brain (Goedert et al. 1991). The major isoform of tau mRNA in oligodendrocytes was found to be tau 1, which represents a marker typical for immature neurons. Tau 2 and tau 4 isoforms were also detected, albeit at a very low level. Immunoblot analysis of oligodendroglia cell extracts confirmed the presence of tau protein. It migrates as a single polypeptide with an apparent molecular weight of approximately 55 kDa. In addition, oligodendrocytes express MAP2c protein, which migrates as a close double band with an apparent molecular weight around 70 kDa. Indirect immunofluorescence staining indicated that tau and MAP2 immunoreactivity was expressed in oligodendrocytes of immature and mature morphologies in the cell somata and cellular processes. Tau was particularly found in the end of the cellular extensions, and both proteins exhibited a distribution similar to myelin basic protein. Thus, oligodendroglia, like neuronal cells, contain microtubule-associated proteins, mainly MAP2c and the tau 1 isoform, although at a much lower level. The presence of these MAPs in myelin-forming cells further points to the functional significance of the cytoskeleton during oligodendrocyte differentiation, process outgrowth, and myelin formation.

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Section: Discussionmentioning

confidence: 94%

Expression of microtubule-associated proteins MAP2 and tau in cultured rat brain oligodendrocytes

Müller

Heinrich

Heck

et al. 1997

Cell and Tissue Research

114

106

View full text Add to dashboard Cite

show abstract

“…Each of these proteins is encoded by a single gene and the RNA transcripts could be alternatively spliced into different isoforms. This is the case for tau (Himmler, 1989), MAP2 (Papandrikopoulou et al, 1989;Langkopf et al, 1994), and MAP1B (Kutschera et al, 1998). So far, the existence of transcripts generated by alternative splicing for MAP1A has not been reported.…”

Section: Introductionmentioning

confidence: 95%

Microtubule‐associated protein 1B function during normal development, regeneration, and pathological conditions in the nervous system

et al. 2003

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Microtubule-associated protein 1B is the first MAP to be expressed during the development of the nervous system. Several different approaches have revealed that MAP1B function is associated with microtubule and actin microfilament polymerization and dynamics. In recent years, the generation of molecular models to inactivate MAP1B function in invertebrates and mammals has sparked some controversy about the real role of MAP1B. Despite discrepancies between some studies, it is clear that MAP1B plays a principal role in the development of the nervous system. In this article, we summarize the evidence for MAP1B function in a wide variety of cellular processes implicated in the proper construction of the nervous system. We also discuss the role of MAP1B in pathological processes.

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“…One can exclude that MAP2a is generated by heavy phosphorylation of MAPZb, because no shift of MAP2a in its electrophoretic mobility is observed after dephosphorylation with alkaline phosphatase (unpublished observations). MAP2a may be generated by alternate splicing of pre-mRNA, as determined in cultured neuronal cells (Langkopf et al, 1994), and high MI MAP2 may exist in several forms that contain four repeats in the microtubule-binding domain.…”

Section: Deve~opment-dependent Changes In Map2 Is0 Formsmentioning

confidence: 99%

Changes of MAP2 phosphorylation during brain development.

Riederer¹,

Dráberová²,

Viklický³

et al. 1995

J Histochem Cytochem.

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The microtubule-associated protein MAP2 is essential for development of early neuronal morphology and maintenance of adult neuronal morphology. Several splice variants exist, MAP2a4, with a lack of MAF' 2a in cat brain. MAP;! is widely used as a neuronal marker. In this study we compared five monoclonal antibodies (MAbs) against MAPZ. They show differences in the immunocytochemical distribution of MAP2 isoforms during development of the visual cortex and cerebellum of the cat. Local and temporal differences were seen with MAb AP18, an antibody directed against a phosphorylation-dependent epitope near the N-terminal end. In large pyramidal dendrites in visual cortex, the AP18 epitope remained in parts immunoreactive after treatment with alkaline phosphatase. Three MAbs, AP14, MT-01, and MT-02, recognized the central region of the M m b molecule, which is not present in MAP2c and 2d, and reacted with phosphotylation-independent epitopes. During the fmt postnatal week the immunostaining in cerebellum differed between antibodies in that some cellular elements in external and internal granular layers and Purkinje cells were stained to various

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Two novel HMW MAP2 variants with four microtubule‐binding repeats and different projection domains

Cited by 14 publications

References 29 publications

Expression of microtubule-associated proteins MAP2 and tau in cultured rat brain oligodendrocytes

Expression of microtubule-associated proteins MAP2 and tau in cultured rat brain oligodendrocytes

Microtubule‐associated protein 1B function during normal development, regeneration, and pathological conditions in the nervous system

Changes of MAP2 phosphorylation during brain development.

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