Two Novel SNPs of<i>PPARγ</i>Significantly Affect Weaning Growth Traits of Nanyang Cattle

Huang, Jieping; Ning-bo, Chen; Li, Xin; An, Shanshan; Zhao, Mingfei; Sun, Taihong; Hao, Ruijie; Ma, Yun

doi:10.1080/10495398.2017.1304950

Cited by 5 publications

(6 citation statements)

References 29 publications

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“…Candidate gene is a useful method to investigate the association between phenotypes and genotypes, which is helpful for improving important economical traits in beef cattle [24,25,26]. Recent data have shown that effective molecular markers used in the selection of beef quality traits could bring benefits in the beef industry [27].…”

Section: Discussionmentioning

confidence: 99%

Two Novel SNPs in RET Gene Are Associated with Cattle Body Measurement Traits

Gao

Huang

Bai

et al. 2019

Animals

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Simple SummaryThe aim of this study was to identify crucial genes and markers potentially associated with cattle economic traits which would provide a basis for molecular marker-assisted breeding and assist in the genetic selection of cattle. The RET gene plays an important role in the development of the gastrointestinal nervous system, which may influence animal body measurement by nutrient absorption. However, there have been no reports on the effects of the RET gene on the body measurement traits of cattle. Two novel SNPs (c.1407A>G and c.1425C>G) were identified in this study, which were significantly associated with the body measurement of two Chinese cattle breeds (Qinchuan and Nanyang cattle). The results suggest that c.1407A>G and c.1425C>G can be used in cattle growth-related traits marker-assisted selection breeding.AbstractThe rearrangement of the transfection (RET) gene, which mediates the functions of the ganglion in the gastrointestinal tract, plays an important role in the development of the gastrointestinal nervous system. Therefore, the RET gene is a potential factor influencing animal body measurement. The aim of this study was to reveal the significant genetic variations in the bovine RET gene and investigate the relationship between genotypes and body measurement in two Chinese cattle breeds (Qinchuan and Nanyang cattle). In this study, two SNPs (c.1407A>G and c.1425C>G) were detected in the exon 7 of RET gene by sequencing. For the SNP1 and SNP2, the GG genotype was significantly associated with body height, hip height, and chest circumference in Qinchuan cattle (p < 0.05). Individuals with an AG-CC genotype showed the lowest value of all body measurement in both breeds. Our results demonstrate that the polymorphisms in the bovine RET gene were significantly associated with body measurement, which could be used as DNA marker on the marker-assisted selection (MAS) and improve the performance of beef cattle.

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Section: Discussionmentioning

confidence: 99%

Two Novel SNPs in RET Gene Are Associated with Cattle Body Measurement Traits

Gao

Huang

Bai

et al. 2019

Animals

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“…Results were presented as means ±SE. Other details can be found in a previous study (Huang et al, 2017). …”

Section: Discussionmentioning

confidence: 99%

“…Samples used in this study were shown in a previous study (Huang et al, 2017). Briefly, seven tissues were collected at the slaughter house for reverse transcription polymerase chain reaction (RT-PCR), including heart, liver, spleen, lung, kidney, muscle, and adipose of three Jiaxian cattle (bullock, 30 months).…”

Section: Samplesmentioning

confidence: 99%

“…Details of the method are shown in a previous study (Huang et al, 2017). Total RNA was reversely transcribed into cDNA using a PrimeScriptsRT reagent kit with gDNA Eraser (TaKaRa, Japan).…”

Section: Expression Analysis Of Ppardmentioning

confidence: 99%

“…The methods for SNP detection and genotyping were as in a previous study (Huang et al, 2017). Pooled DNA samples were used as a PCR template for SNP detection.…”

Section: Snp Detection and Genotypingmentioning

confidence: 99%

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A combined genotype of three SNPs in the bovine gene is related to growth performance in Chinese cattle

et al. 2017

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Abstract. PPARD is involved in multiple biological processes, especially for those associated with energy metabolism. PPARD regulates lipid metabolism through up-regulate expression of genes associating with adipogenesis. This makes PPARD a significant candidate gene for production traits of livestock animals. Association studies between PPARD polymorphisms and production traits have been reported in pigs but are limited for other animals, including cattle. Here, we investigated the expression profile and polymorphism of bovine PPARD as well as their association with growth traits in Chinese cattle. Our results showed that the highest expression of PPARD was detected in kidney, following by adipose, which is consistent with its involvement in energy metabolism. Three SNPs of PPARD were detected and used to undergo selection pressure according the result of Hardy-Weinberg equilibrium analysis (P < 0.05). Moreover, all of these SNPs showed moderate diversity (0.25 < PIC < 0.5), indicating their relatively high selection potential. Association analysis suggested that individuals with the GAAGTT combined genotype of three SNPs detected showed optimal values in all of the growth traits analyzed. These results revealed that the GAAGTT combined genotype of three SNPs detected in the bovine PPARD gene was a significant potential genetic marker for marker-assisted selection in Chinese cattle. However, this should be further verified in larger populations before being applied to breeding.

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