Two Nuclear Export Signals Specify the Cytoplasmic Localization of Calcineurin B Homologous Protein 1

Nagita, Mana; Inoue, Hiroki; Nakamura, Norihiro; Kanazawa, Hiroshi

doi:10.1093/jb/mvg223

Cited by 8 publications

(9 citation statements)

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“…However, in A. nidulans, there are several arguments against this hypothesis: (i) computer analysis † of the amino acid sequence of both HapC and HapE did not reveal any obvious NES sequence. 29 The same was concluded on the basis of the NES consensus sequence defined by Nagita et al 30 However, since NESs are poorly defined, the HapC amino acid sequence shows a region of some similarity to the NES consensus found for Nap1p from S. cerevisiae, 29 whereas HapE does not contain such a sequence. Even if the putative HapC NES were functional, it would be present in HapC and not HapE and therefore, its presence would not support the hypothesis of an NES present in a HapE homolog.…”

Section: Discussionsupporting

confidence: 55%

A Single Subunit of a Heterotrimeric CCAAT-binding Complex Carries a Nuclear Localization Signal: Piggy Back Transport of the Pre-assembled Complex to the Nucleus

Steidl

Tüncher

Goda

et al. 2004

Journal of Molecular Biology

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Section: Discussionsupporting

confidence: 55%

A Single Subunit of a Heterotrimeric CCAAT-binding Complex Carries a Nuclear Localization Signal: Piggy Back Transport of the Pre-assembled Complex to the Nucleus

Steidl

Tüncher

Goda

et al. 2004

Journal of Molecular Biology

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“…Although it is well established that the dephosphorylation of Crz1p by calcineurin leads to its nuclear translocation (Stathopoulos‐Gerontides et al ., 1999), there are no reports of the nuclear localization of calcineurin B in the yeasts or filamentous fungi. Nuclear localization of a calcineurin B homologous protein (CHP1) has been reported (Nagita et al ., 2003; Jiménez‐Vidal et al ., 2010). We could not identify any potential NLS sequence in the A. fumigatus CnaB and it is possible that CnaB may translocate into the nucleus with CnaA and CrzA.…”

Section: Discussionmentioning

confidence: 99%

Localization and activity of the calcineurin catalytic and regulatory subunit complex at the septum is essential for hyphal elongation and proper septation in Aspergillus fumigatus

Juvvadi

Fortwendel

Rogg

et al. 2011

Molecular Microbiology

115

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Summary Calcineurin, a heterodimer composed of the catalytic (CnaA) and regulatory (CnaB) subunits, plays key roles in growth, virulence, and stress responses of fungi. To investigate the contribution of CnaA and CnaB to hyphal growth and septation, ΔcnaB and ΔcnaA ΔcnaB strains of A. fumigatus were constructed. CnaA co-localizes to the contractile actin ring early during septation and remains at the center of the mature septum. While CnaB's septal localization is CnaA-dependent, CnaA's septal localization is CnaB-independent but CnaB is required for CnaA's function at the septum. Catalytic null mutations in CnaA caused stunted growth despite septal localization of the calcineurin complex, indicating the requirement of calcineurin activity at the septum. Compared to the ΔcnaA and ΔcnaB strains, the ΔcnaA ΔcnaB strain displayed more defective growth and aberrant septation. While three Ca2+-binding motifs in CnaB were sufficient for its association with CnaA at the septum, the amino-terminal arginine-rich domains (16-RRRR-19 and 44-RLRKR-48) are dispensable for septal localization, yet required for complete functionality. Mutation of the 51-KLDK-54 motif in CnaB causes its mislocalization from the septum to the nucleus, suggesting it is a nuclear export signal sequence. These findings confirm a cooperative role for calcineurin complex in regulating hyphal growth and septation.

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“…1996). Only one functional NES (aa 137–148) was identified in CHP2, although several studies have reported two putative NES (Nagita et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

Nuclear accumulation of Calcineurin B Homologous Protein 2 (CHP2) results in enhanced proliferation of tumor cells

Li¹,

Wang²,

Lin

et al. 2011

Genes to Cells

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The interaction between calcineurin B homologous protein 2 (CHP2) and Na + ⁄ H + exchanger 1 (NHE1), two membrane proteins, is essential for protecting cells from serum deprivationinduced death. Although four putative EF-hands in CHP2 had been predicted for years, Ca 2+ -binding activities of these motifs have not been tested yet, their role in this process remain poorly understood. To identify Ca 2+ -binding motifs required for the stable formation of CHP2 ⁄ NHE1 complexes, we developed a mutagenesis-based assay in PS120 cells. We found that 45 Ca 2+ bond to two EF-hand motifs (EF3 and 4) of CHP2 proteins with high affinity. Complex formation between CHP2 and the CHP2 binding domain of NHE1 resulted in a marked increase in the Ca 2+ -binding affinity of CHP2. Co-immunoprecipitation and distribution of GFP-tagged CHP2-EF3m ⁄ 4m also indicated that Ca 2+ affected the membrane location of CHP2 to interact with NHE1. The C-terminal region of CHP2 contains a nuclear export sequence (NES). When the six leucines of NES were mutated to alanines, the resulting CHP2 protein was predominantly localized to the nucleus. Furthermore, mutation of the NES resulted in enhanced proliferation and oncogenic potential of HeLa cells. Together, these results show that calcium and NES control the subcellular distribution of CHP2 and then distinctively regulate cell proliferation.

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Two Nuclear Export Signals Specify the Cytoplasmic Localization of Calcineurin B Homologous Protein 1

Cited by 8 publications

References 0 publications

A Single Subunit of a Heterotrimeric CCAAT-binding Complex Carries a Nuclear Localization Signal: Piggy Back Transport of the Pre-assembled Complex to the Nucleus

A Single Subunit of a Heterotrimeric CCAAT-binding Complex Carries a Nuclear Localization Signal: Piggy Back Transport of the Pre-assembled Complex to the Nucleus

Localization and activity of the calcineurin catalytic and regulatory subunit complex at the septum is essential for hyphal elongation and proper septation in Aspergillus fumigatus

Nuclear accumulation of Calcineurin B Homologous Protein 2 (CHP2) results in enhanced proliferation of tumor cells

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