Two of three promoter elements identified in a rice gene for proliferating cell nuclear antigen are essential for meristematic tissue‐specific expression

Kosugi, Shunichi; Suzuka, Iwao; Ohashi, Yuko

doi:10.1046/j.1365-313x.1995.07060877.x

Cited by 71 publications

(85 citation statements)

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“…This suggests that sites Ila and Ilb most probably function as positive cisacting elements in proliferating cells. In addition, results of electrophoretic mobility shift assays (EMSAs) have also shown that these sites bind to nuclear proteins with identical or similar DNA binding specificity in rice and tobacco (Kosugi et al, 1995). These data suggest that sites Ila and Ilb have a similar function in diversified species.…”

Section: Introductionmentioning

confidence: 86%

“…We have previously defined the promoter elements (site Ila and site llb) essential for meristematic tissue-specific expression of the rice PCNA gene (Kosugi et al, 1995). In this study, we isolated cDNAs for specific binding proteins for sites Ila and Ilb.…”

Section: Cloning Of the Pcfl And Pcf2 Cdnasmentioning

confidence: 99%

“…These observations indicate that PCFl and PCF2 are DNA binding proteins specific for sites Ila and Ilb and that the DNA binding specificity and affinity of PCFl are similar to that of PCF2. lntroduction of this mutated sequence in the rice PCNA promoter leads to a loss of the promoter activity (Kosugi et al, 1995).…”

Section: Cloning Of the Pcfl And Pcf2 Cdnasmentioning

confidence: 99%

“…The PCNA protein is also present in proliferating cells but absent from nondividing cells and terminally differentiated plant tissues (Daidoji et al, 1992). Loss-of-function analysis of the rice PCNA promoter using transgenic plants has demonstrated that two elements (sites Ila and llb) in the proximal region are essential for the proliferating cell-specific transcriptional activity (Kosugi et al, 1995). On the other hand, two repeatedsite Ila sequences located upstream of the cauliflower mosaic virus 35s minimal promoter confer transcriptional activation in tobacco protoplasts (Kosugi et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene.

1997

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We have previously defined the promoter elements, sites Ila and Ilb, in the rice proliferating cell nuclear antigen (PCNA) gene that are essential for meristematic tissue-specific expression. In this study, we isolated and characterized cDNAs encoding proteins that specifically bind to sites Ila and Ilb. The two DNA binding proteins, designated PCFl and PCFP, share >To% homology in common conserved sequences at the N-terminal regions. The conserved regions are responsible for DNA binding and homodimer and heterodimer formation, and they contain a putative basic helix-loop-helix (bHLH) motif. The structure and DNA binding specificity of the bHLH motif are distinguishable from those of other known bHLH proteins that bind to the E-box. The motif is >70% homologous t o several expressed sequence tags from Arabidopsis and rice, suggesting that PCFl and PCF2 are members of a nove1 family of proteins that are conserved in monocotyledons and dicotyledons. A supershift assay using an anti-PCF2 antibody showed the involvement of PCF2 in site Ila (site Ilb) binding activities in rice nuclear extracts, particularly in meristematic tissues. PCFl and PCFS were also more likely t o form heterodimers than homodimers. Our results suggest that PCFl and PCFS are involved in meristematic tissue-specific expression of the rice PCNA gene through binding to sites Ila and Ilb and formation of homodimers or heterodimers.

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Section: Introductionmentioning

confidence: 86%

Section: Cloning Of the Pcfl And Pcf2 Cdnasmentioning

confidence: 99%

Section: Cloning Of the Pcfl And Pcf2 Cdnasmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene.

1997

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“…Two families of proteins that bind to the CRT in promoters of genes responding to these stresses are C repeat binding factors (CBFs; also known as DRE binding factor 1s [DREB1s]) and DREB2s (Jaglo-Ottosen et al, 1998;Liu et al, 1998). The sequence AGGCCCAT ( Figure 2D) contains the core of a sequence originally described as one of three cis-acting elements in the promoter of the proliferating cell nuclear antigen (PNCA) gene, originally in rice (Oryza sativa), which was thus designated Site IIa (Kosugi et al, 1995). Rice proteins identified as binding to this motif include bHLH transcription factors PCF1 and PCF2 (Kosugi and Ohashi, 1997).…”

Section: Imposed [Ca 2+ ] C Elevations Are Capable Of Inducing Signifmentioning

confidence: 99%

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

et al. 2011

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Increases in intracellular calcium concentration ([Ca 2+ ] c ) mediate plant responses to stress by regulating the expression of genes encoding proteins that confer tolerance. Several plant stress genes have previously been shown to be calciumregulated, and in one case, a specific promoter motif Abscisic Acid Responsive-Element (ABRE) has been found to be regulated by calcium. A comprehensive survey of the Arabidopsis thaliana transcriptome for calcium-regulated promoter motifs was performed by measuring the expression of genes in Arabidopsis seedlings responding to three calcium elevations of different characteristics, using full genome microarray analysis. This work revealed a total of 269 genes upregulated by [Ca 2+ ] c in Arabidopsis. Bioinformatic analysis strongly indicated that at least four promoter motifs were [Ca 2+ ] c -regulated in planta. We confirmed this finding by expressing in plants chimeric gene constructs controlled exclusively by these cis-elements and by testing the necessity and sufficiency of calcium for their expression. Our data reveal that the C-Repeat/Drought-Responsive Element, Site II, and CAM box (along with the previously identified ABRE) promoter motifs are calcium-regulated. The identification of these promoter elements targeted by the second messenger intracellular calcium has implications for plant signaling in response to a variety of stimuli, including cold, drought, and biotic stress.

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Identification of a Sequence Element Involved in AC2-Mediated Transactivation of the Pepper Huasteco Virus Coat Protein Gene

et al. 1999

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The geminivirus AC2 gene product transactivates the expression of the coat and movement protein (CP and BV1) genes, and this effect seems to be mediated by specific although hitherto unknown cis-acting elements. In this work we examined regions from the CP and BV1 gene promoters of pepper huasteco virus (PHV) to define the sequence elements involved in regulation by AC2. Results from transient gene expression and transgenic plant assays suggest that a truncated 115-nt CP promoter is still responsive to the viral transactivator. This promoter contains three elements similar to a sequence motif termed conserved late element (CLE), which is found in the regulatory regions of many geminiviruses and that was previously suggested, on a theoretical basis, to be a potential functional target for AC2 (Argüello-Astorga et al. (1994), Virology 203, 90-100). To confirm these results, an oligonucleotide containing two CLE motifs was synthesized and characterized in gain-of-function experiments. Transient expression assays showed that this 29-nt sequence is able to confer AC2 responsiveness to heterologous promoters. A smaller oligonucleotide (16 nt) containing a single CLE also conferred this activity. In addition, when the CLE motifs were mutated in their original context (truncated 115-nt promoter), this modified promoter lost its ability to be transactivated by AC2. All these results support the involvement, at least in the case of PHV, of CLE sequences in the process of transactivation.

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Two of three promoter elements identified in a rice gene for proliferating cell nuclear antigen are essential for meristematic tissue‐specific expression

Cited by 71 publications

References 0 publications

PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene.

PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene.

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

Identification of a Sequence Element Involved in AC2-Mediated Transactivation of the Pepper Huasteco Virus Coat Protein Gene

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