Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales

Azuma, Kyoichi; Kobayashi, Masaki; Nakamura, Masahisa; Suzuki, Nobuo; Yashima, Sayaka; Iwamuro, Shawichi; Ikegame, Mika; Yamamoto, Tetsuro; Hattori, Atsuhiko

doi:10.1016/j.bbrc.2007.08.010

Cited by 51 publications

(47 citation statements)

References 27 publications

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“…In the past, our laboratory has sequenced TRAP and cathepsin K from the goldfish scale [33]. In the scale osteoclasts, the osteoclast markers TRAP and cathepsin K were expressed which were detected by in situ hybridization [33].…”

Section: Discussionmentioning

confidence: 99%

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Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Suzuki

Danks

Maruyama

et al. 2011

Bone

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The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100 pg/ml to 10 ng/ml) significantly increased ALP activity at 6 h of incubation. High-dose (10 ng/ml) fugu PTH1 significantly increased ALP activity even after 18 h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6 h of incubation, but fugu PTH1 (100 pg/ml to 10 ng/ml) significantly increased TRAP activity at 18 h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-B ligand in osteoblasts and the receptor activator NF-B mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.3

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Section: Discussionmentioning

confidence: 99%

“…In the scale osteoclasts, the osteoclast markers TRAP and cathepsin K were expressed which were detected by in situ hybridization [33]. In osteoblasts as well, type I collagen [12], osteocalcin [13], and osteonectin [14,15] are present in the scales.…”

Section: Discussionmentioning

confidence: 99%

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Suzuki

Danks

Maruyama

et al. 2011

Bone

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“…In addition, the increase in TRAP activity was greater than the increase in ALP activity. Tartrateresistant acid phosphatase is a specific marker for osteoclasts in mammals (Helfrich, et al, 1987;1989;Lee et al, 1995), and TRAP mRNA is expressed in teleost scales (Azuma, et al, 2007). Multinucleated giant cells with a well-developed ruffled border, which is a characteristic feature of mammalian activated osteoclasts, have been found on the pit of goldfish scales (Azuma, et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Effects of Low-Intensity Ultrasound on Osteoblasts and Osteoclasts in Goldfish Scale

Kitamura

2010

Biol. Sci. Space

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Teleost scales are calcified tissue that contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in human bone. Recently, a new culture system has been developed for the evaluation of bone metabolism using goldfish scales with alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) as markers of osteoblasts and osteoclasts, respe ctively. Using this system, the activities of ALP and TRAP in ontogenic scales were measured after removal of almost all scales on one side of the body. On both days 7 and 10 after removal, the remaining ontogenic scales showed significantly higher TRAP and ALP activity than normal ontogenic scales (p<0.01). Using the remaining ontogenic scales, the effects of low-intensity ultrasound on ALP and TRAP activity were investigated. Alkaline phosphatase activity was significantly increased (p<0.05) 18 and 24 h after ultrasound treatment. In contrast, the TRAP activity in the ultrasound-treated scales was significantly suppressed (p<0.01) after incubation for 24 or 48 h. The results of the present study reveal that low-intensity ultrasound inhibits osteoclast activity in a native calcified tissue environment in which osteoblasts and osteoclasts with increased activity coexist.

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“…The teleost scale is calcified tissue that contains osteoblasts, osteoclasts, and the two layers of bone matrix, i.e., a bony layer, which is a thin, well-calcified external layer, and a fibrillary layer, which is a thick, partially calcified layer (Bereiter-Hahn and Zylberberg, 1993;Suzuki et al, 2000;Yoshikubo et al, 2005;Suzuki et al, 2007;Azuma et al, 2007;Ohira et al, 2007). The bone matrix, which includes type I collagen (Zylberberg et al, 1992), osteocalcin (Nishimoto et al, 1992), and hydroxyapatite (Onozato et al, 1979), is present in scale as well as in mammalian bone.…”

Section: Introductionmentioning

confidence: 99%

Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish,Carassius auratus

Suzuki¹,

Sato²,

Nassar³

et al. 2016

Zoological Science

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We have developed an original in vitro bioassay using teleost scale that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from 2 sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We can detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added into culture medium at dilution rates of 50, 100, and 500 and incubated with the goldfish scales for 6 hrs. Thereafter, ALP and TRAP activities were measured. As a result, ALP activity was significantly suppressed by both polluted seawater samples diluted at least 500 times, although TRAP activity did not change. In addition, mRNA expressions of osteoblastic markers (ALP, osteocalcin, and the receptor activator of the NF-B ligand) decreased significantly, as did the ALP enzyme activity. Actually, ALP activity decreased with selected PAHs and NPAHs treatments. We conclude that seawater polluted with highly concentrated PAHs and NPAHs influenced bone metabolism in teleosts.

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Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales

Cited by 51 publications

References 27 publications

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Effects of Low-Intensity Ultrasound on Osteoblasts and Osteoclasts in Goldfish Scale

Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish,Carassius auratus

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