Two pathways of 2n gamete formation and differences in the frequencies of 2n gametes between wild species and interspecific hybrids

Kondo, Haruka; Deguchi, Ayumi; Kikuchi, Shinichi; Miyoshi, K.

doi:10.1007/s00299-022-02915-5

Plant Cell Rep

2022

DOI: 10.1007/s00299-022-02915-5

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Two pathways of 2n gamete formation and differences in the frequencies of 2n gametes between wild species and interspecific hybrids

Haruka Kondo

Ayumi Deguchi

Shinichi Kikuchi

et al.

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2024

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Cited by 3 publications

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“…The cross compatibility between different species was reported to be high, and even an increasing number of interspecific and intergeneric hybrids has been obtained through artificial pollination (Li et al, 2014;Ogura-Tsujita et al, 2014;Joffard et al, 2022). These interspecific hybrids have a high percentage in occurrence of 2n gametes and have been successfully employed to create sexual polyploids in breeding programs (Zeng et al, 2020;Kondo et al, 2022). However, because of reproductive isolation caused by geographical, temporal, and spatial isolations, the interspecific hybrids are rare in nature.…”

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Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants

Su²,

et al. 2022

Front. Plant Sci.

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Triploid is considered a reproductive barrier and also a bridge in the formation of polyploids. However, few reports are available in Cymbidium. In this study, diploid ‘Xiaofeng’, sexual triploid ‘Yuchan’ and ‘Huanghe’ of Cymbidium were used to evaluate hybridization compatibility of the triploids. Results showed that the sexual triploids were fertile whether they were used as male or female parents. ‘Yuchan’ produced male gametes of 1x, 1x~2x, 2x, 2x~3x, and 3x at frequencies of 8.89%, 77.78%, 6.67%, 3.33%, and 3.33%, respectively; while ‘Huanghe’ produced 3.33% 1x, 80.00% 1x~2x, 8.89% 2x, 5.56% 2x~3x, and 2.22% 3x male gametes. The cross of ‘Xiaofeng’ with ‘Yuchan’ produced progenies with a wide range of ploidy levels, including one diploid, 34 2×~3× aneuploids, 12 triploids, and one tetraploid, indicating that male gametes produced by sexual triploid were fertile and could be transmitted and fused with egg cells. On the other hand, 10 progenies obtained from the cross of ‘Yuchan’ × ‘Xiaofeng’ were all aneuploids. The cross of ‘Yuchan’ with ‘Huanghe’ produced 40 progenies including three 2×~3× aneuploids, nine 3×~4× aneuploids, 21 tetraploids, six 4×~5× aneuploids, and one pentaploid, suggesting that 2x gametes, instead of the unreduced ones played a more important role in the formation of tetraploids. The survival rates of the hybrids were all above 80.00%, with the tetraploids at 96.67%. Cytological analysis revealed that during meiosis of sexual polyploids, two chromosome sets of the 2n gamete were inclined to enter into the same daughter cell, resulting in the production of 2x gametes. Our results indicate that the triploid cymbidiums are not reproductive barrier but serve as a bridge in the formation of polyploid plants.

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confidence: 99%

Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants

Su²,

et al. 2022

Front. Plant Sci.

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Exploring the patterns of evolution: Core thoughts and focus on the saltational model

Usai,

Fambrini,

Pugliesi

et al. 2024

BioSystems

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Chromosomal analysis of progenies between Lilium intersectional hybrids and wild species using ND-FISH and GISH

Zhou,

Yong,

Zhu

et al. 2024

Front. Plant Sci.

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IntroductionIntersectional hybrids in lilies possess significant breeding value, but the lack of complete lily genomes and complex genotypes pose challenges for early identification of lily hybrids. This study aimed to use intersectional hybrid cultivars as female parents and wild lilies as male parents to facilitate early identification of hybrid offsprings and enhance the efficiency and convenience of the process.MethodsWe investigated the nature of cross combinations using Non-denaturing Fluorescence In Situ Hybridization (ND-FISH) and Genomic In Situ Hybridization (GISH) techniques. Three novel oligonucleotide probes—Oligo-pTa794, Oligo-pITS and Oligo-telo—were developed for lily chromosome research.ResultsOur results demonstrated successful hybridization between wild lilies and intersectional hybrid cultivars, producing a total of 130 hybrid progenies. The combination of ND-FISH and GISH analyses effectively revealed the genomic composition of the hybrid progeny and determined the parental origin of specific chromosomes.DiscussionThis research provides significant guidance for lily breeding practices and offers a valuable reference for the application of ND-FISH and GISH techniques in interspecific hybridization breeding and molecular cytogenetic research across various plant species. The methods developed enable more precise, efficient, and convenient identification of hybrid offsprings.

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Two pathways of 2n gamete formation and differences in the frequencies of 2n gametes between wild species and interspecific hybrids

Cited by 3 publications

References 60 publications

Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants

Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants

Exploring the patterns of evolution: Core thoughts and focus on the saltational model

Chromosomal analysis of progenies between Lilium intersectional hybrids and wild species using ND-FISH and GISH

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