Two phase microalgae growth in the open system for enhanced lipid productivity

Das, Probir; Aziz, Siti Sarah; Obbard, Jeffrey Philip

doi:10.1016/j.renene.2011.02.002

Cited by 124 publications

(50 citation statements)

References 22 publications

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“…Yen and Chang (2013) similarly reported higher biomass concentration in sequential photoautotrophic-mixotrophic cultures; however, the linoleic acid content (18:1) was the same when compared with photoautotrophic cultures. Das et al (2011) studied intracellular lipid accumulation by Nannochloropsis sp. in sequential photoautotrophic-mixotrophic cultures.…”

Section: Sequential Photoautotrophic-mixotrophic Culturesmentioning

confidence: 99%

Culture Systems Incorporating Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae

Ogbonna

McHenry

2015

Biofuel and Biorefinery Technologies

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The feasibility of using various culture systems incorporating heterotrophic metabolism for biodiesel oil production was compared. Heterotrophic culture can be used to achieve high cell concentration, and depending on the strain and organic carbon source employed, the introduction of light (mixotrophic culture) can enhance cell growth and oil accumulation. However, mixotrophic cultures also face the problem of light limitation, and depending on the relative concentrations of the organic carbon source and light intensity, the interaction between the heterotrophic and photoautotrophic metabolic activities can have negative effects on cell growth and oil accumulation. Systems that separate the two metabolic activities in time or space, such as cyclic photoautotrophic-heterotrophic cultures, sequential heterotrophic-photoautotrophic cultures, and sequential photoautotrophic-mixotrophic cultures, can all be used to improve oil productivity. However, the effectiveness of each system depends on the strain of microalgae and other culture conditions.

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Section: Sequential Photoautotrophic-mixotrophic Culturesmentioning

confidence: 99%

Culture Systems Incorporating Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae

Ogbonna

McHenry

2015

Biofuel and Biorefinery Technologies

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“…The carbon source that gives high biomass productivity may not be the one that gives high oil production. Although for many strains, glucose has been reported to be the best in terms of cell growth, Das et al (2011) ranked the effectiveness of different organic substrates in terms of intracellular lipid contents in mixotrophic culture in the following order: glycerol > sucrose > glucose.…”

Section: Organic Carbon Sources For Heterotrophic and Mixotrophic Culmentioning

confidence: 99%

Potentials of Exploiting Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae

Ogbonna

Moheimani

2015

Biofuel and Biorefinery Technologies

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The current prices of microalgae oils are much higher than oils from higher plants (vegetable oils) mainly due to the high cost of photoautotrophic cultivation of microalgae. However, many strains of microalgae can also grow and produce oil using organic carbons, as the carbon source under dark (heterotrophy) or light conditions (mixotrophy). Lipid productivities of most strains of microalgae are higher in culture systems that incorporate heterotrophic metabolisms (presence of organic carbon source) than under photoautotrophic conditions. This is because for many strains, cell growth rates and final cell concentrations are higher in heterotrophic cultures than in photoautotrophic cultures. Furthermore, in some cases, the oil contents of the cells are also higher in cultures incorporating heterotrophic metabolisms. It has also been reported for some strains that the quality of oil produced in the presence of organic carbon sources are more suitable for biodiesel oil production than those produced under photoautotrophic conditions. Thus, heterotrophy can be used to reduce the cost of biodiesel oil production, but the effectiveness of the various organic carbons in supporting cell growth and oil accumulation depends on the strain and other culture conditions. Use of wastewaters for cultivation of microalgae can further substantially reduce the cost of production (since they contain carbon, nitrogen, and other nutrients) and also reduce the requirement for freshwater. Generally, many factors such as nitrogen limitation, phosphate limitation, silicon limitation, control of pH, and low temperature can be used to increase oil accumulation, although their effectiveness depends on the strain and other culture conditions.

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“…Moreover, P. tricornutum [13] and Nannochloropsis sp. [18] grow faster in mixotrophy with glycerol as a carbon source than with any other organic carbons. In other studies, however, glucose could enhance the growth of Cyclotella cryptica [10], Tetraselmis suecica [11], and Chlorella vulgaris [6] in heterotrophic culture, but this is at odds with our results.…”

Section: Discussionmentioning

confidence: 99%

Cultivation ofIsochrysis galbanain Phototrophic, Heterotrophic, and Mixotrophic Conditions

Alkhamis

Qin

2013

BioMed Research International

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This study compared the growth and biomass production of Isochrysis galbana under hetero-, mixo-, and phototrophic conditions using different organic carbon sources. The growth of I. galbana was inhibited in heterotrophy but was enhanced in mixotrophy compared to that in phototrophy. Subsequently, the influences of organic carbon and environmental factors (light and salinity) on the growth of I. galbana were further investigated. Algal dry weight increased as glycerol concentrations increased from 0 to 200 mmol and the highest algal production occurred at 50 mmol glycerol. At a range of light intensities of 25–200 μmol photons m−2 s−2, the highest algal growth rate occurred at 100 photons μmol m−2 s−2. The growth of I. galbana was significantly affected by photoperiod, and the maximal dry weight was obtained at 12 h light and 12 h dark. In the salinity test, I. galbana could grow in a wide range of salinities from 10 to 65‰, but the 35‰ salinity was optimal. This study suggests that the growth and production of I. galbana can be improved using mixotrophic culture at 50 mmol glycerol in 35‰ salinity.

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Two phase microalgae growth in the open system for enhanced lipid productivity

Cited by 124 publications

References 22 publications

Culture Systems Incorporating Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae

Culture Systems Incorporating Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae

Potentials of Exploiting Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae

Cultivation ofIsochrysis galbanain Phototrophic, Heterotrophic, and Mixotrophic Conditions

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