Two-photon excitation nanoparticles for photodynamic therapy

Shen, Yizhong; Shuhendler, Adam J.; Ye, Deju; Xu, Jing‐Juan; Chen, Hong‐Yuan

doi:10.1039/c6cs00442c

Cited by 494 publications

(308 citation statements)

References 178 publications

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“…Several inorganic NPs, including metallic NPs (Wang et al, 2017), inorganic nanocrystals (Ipe et al, 2005; Wang et al, 2015) and magnetic NPs, have been reported to have sizable effects on the ROS population, thus representing an interesting strategy for photothermal therapies (Ye and Chen, 2016; Dayem et al, 2017). Among carbon-based materials, carbon nanotubes, graphene and polymer nano-bioconjugates can also sustain photo-thermal stimulation (Miyako et al, 2014; Chechetka et al, 2016; Lyu et al, 2016; Li et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Photocatalytic Activity of Polymer Nanoparticles Modulates Intracellular Calcium Dynamics and Reactive Oxygen Species in HEK-293 Cells

Bossio

Aziz

Tullii

et al. 2018

Front. Bioeng. Biotechnol.

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Optical modulation of living cells activity by light-absorbing exogenous materials is gaining increasing interest, due to the possibility both to achieve high spatial and temporal resolution with a minimally invasive and reversible technique and to avoid the need of viral transfection with light-sensitive proteins. In this context, conjugated polymers represent ideal candidates for photo-transduction, due to their excellent optoelectronic and biocompatibility properties. In this work, we demonstrate that organic polymer nanoparticles, based on poly(3-hexylthiophene) conjugated polymer, establish a functional interaction with an in vitro cell model (Human Embryonic Kidney cells, HEK-293). They display photocatalytic activity in aqueous environment and, once internalized within the cell cytosol, efficiently generate reactive oxygen species (ROS) upon visible light excitation, without affecting cell viability. Interestingly, light-activated ROS generation deterministically triggers modulation of intracellular calcium ion flux, successfully controlled at the single cell level. In perspective, the capability of polymer NPs to produce ROS and to modulate Ca2+ dynamics by illumination on-demand, at non-toxic levels, may open the path to the study of biological processes with a gene-less approach and unprecedented spatio-temporal resolution, as well as to the development of new biotechnology tools for cell optical modulation.

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Section: Discussionmentioning

confidence: 99%

Photocatalytic Activity of Polymer Nanoparticles Modulates Intracellular Calcium Dynamics and Reactive Oxygen Species in HEK-293 Cells

Bossio

Aziz

Tullii

et al. 2018

Front. Bioeng. Biotechnol.

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“…[4] As far as PDT is concerned, oxygen is known to be essential in the process of singlet oxygen generation, while tumor hypoxia microenvir-onment considerably limits the therapeutic effect of PDT. [9] However,h ow to combine these techniques into one system for solving both problems PDT face is still ag reat challenge. [7] Researchers have proposed many solutions to solve these problems,i ncluding increasing oxygen supply inside tumor to enhance PDT efficiency, [8] applying two-photon excitation (TPE) to improve treatment depth in PDT.…”

mentioning

confidence: 99%

An Assembled Nanocomplex for Improving both Therapeutic Efficiency and Treatment Depth in Photodynamic Therapy

et al. 2018

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Photodynamic therapy (PDT) shows unique selectivity and irreversible destruction toward treated tissues or cells, but still has several problems in clinical practice. One is limited therapeutic efficiency, which is attributed to hypoxia in tumor sites. Another is the limited treatment depth because traditional photosensitizes are excited by short wavelength light (<700 nm). An assembled nano-complex system composed of oxygen donor, two-photon absorption (TPA) species, and photosensitizer (PS) was synthesized to address both problems. The photosensitizer is excited indirectly by two-photon laser through intraparticle FRET mechanism for improving treatment depth. The oxygen donor, hemoglobin, can supply extra oxygen into tumor location through targeting effect for enhanced PDT efficiency. The mechanism and PDT effect were verified through both in vitro and in vivo experiments. The simple system is promising to promote two-photon PDT for clinical applications.

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“…This technique combine the advantages of TPE near-infrared (NIR) photosensitizers and nanotechnology and has been reviewed by Shen et al [466]. The absorption of two relatively low-energy NIR photons will enable the emission of high-energy photons in the visible spectrum, which in its turn will sensitize oxygen producing singlet oxygen and reactive oxygen species (ROS) able to kill targeted cancer cells due to its cytotoxic effect.…”

Section: Multimodal Therapy Options For Prostate and Breast Cancermentioning

confidence: 99%

Nanoparticles as Theranostic Vehicles in Experimental and Clinical Applications—Focus on Prostate and Breast Cancer

Elgqvist

2017

IJMS

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Prostate and breast cancer are the second most and most commonly diagnosed cancer in men and women worldwide, respectively. The American Cancer Society estimates that during 2016 in the USA around 430,000 individuals were diagnosed with one of these two types of cancers, and approximately 15% of them will die from the disease. In Europe, the rate of incidences and deaths are similar to those in the USA. Several different more or less successful diagnostic and therapeutic approaches have been developed and evaluated in order to tackle this issue and thereby decrease the death rates. By using nanoparticles as vehicles carrying both diagnostic and therapeutic molecular entities, individualized targeted theranostic nanomedicine has emerged as a promising option to increase the sensitivity and the specificity during diagnosis, as well as the likelihood of survival or prolonged survival after therapy. This article presents and discusses important and promising different kinds of nanoparticles, as well as imaging and therapy options, suitable for theranostic applications. The presentation of different nanoparticles and theranostic applications is quite general, but there is a special focus on prostate cancer. Some references and aspects regarding breast cancer are however also presented and discussed. Finally, the prostate cancer case is presented in more detail regarding diagnosis, staging, recurrence, metastases, and treatment options available today, followed by possible ways to move forward applying theranostics for both prostate and breast cancer based on promising experiments performed until today.

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Two-photon excitation nanoparticles for photodynamic therapy

Cited by 494 publications

References 178 publications

Photocatalytic Activity of Polymer Nanoparticles Modulates Intracellular Calcium Dynamics and Reactive Oxygen Species in HEK-293 Cells

Photocatalytic Activity of Polymer Nanoparticles Modulates Intracellular Calcium Dynamics and Reactive Oxygen Species in HEK-293 Cells

An Assembled Nanocomplex for Improving both Therapeutic Efficiency and Treatment Depth in Photodynamic Therapy

Nanoparticles as Theranostic Vehicles in Experimental and Clinical Applications—Focus on Prostate and Breast Cancer

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