Two-photon excited fluorescence imaging of the pancreatic solid pseudopapillary tumor without hematoxylin and eosin stains

Abstract

Solid pseudopapillary tumor (SPT) of the pancreas is an epithelial tumor with low-grade malignant potential and present more common in females. At present, the gold standard for accurate diagnosis of pancreatic tumor was mostly depending on the pathological and/or cytological evaluation. In this work, TPEF microscopy was applied to obtain the images of human normal pancreas and SPT of the pancreas without hematoxylin and eosin (H&E) staining, for the purpose of identifying the organization structural, cell morphological, and cytoplasm changing, which were then compared to their corresponding H&E stained histopathological results. Our results showed that high-resolution TPEF imaging of the pancreatic SPT can clearly distinguish the pathological features from normal pancreas in unstained histological sections, and the results are consistent with the histological results. Moreover, we measured the nuclear-cytoplasmic ratios of the pancreatic SPT and normal pancreas to characterize their difference in the cytomorphological feature. It indicated that this technique can achieve the consistent information of pathological diagnosis, and has the potential to substantially improve the optical diagnosis and treatment of the pancreatic SPT without H&E staining in the future. SCANNING 38:245-250, 2016. © 2015 Wiley Periodicals, Inc.

Practical two‐photon‐absorption cross sections and spectra of eosin and hematoxylin

“…Moreover eosin and hematoxylin stained samples can be in principle inspected also by using twophoton fluorescence microscopy. The advantages of this technique are the intrinsic high longitudinal resolution, low photobleaching, suitability for three-dimensional reconstruction, low phototoxicity, higher penetration depth with respect to one-photon fluorescence [21][22][23][24]. Indeed several investigations on eosin and hematoxylin stained tissues have been carried out [25][26][27]33].…”