Two-photon excited fluorescence of the lens for the diagnosis of presbyopia

Steiner, Rudolf; Keßler, Mirjam; Fugger, Oliver; Dolp, Frank; Russ, Detlef

doi:10.1134/s0030400x09090276

Cited by 1 publication

(3 citation statements)

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“…The mean lifetime of the AF of this layer was (1.42 ±0.10) nanoseconds (Table ). A similar value was reported for superficial cortex fibers of mouse lenses .…”

Section: Resultssupporting

confidence: 88%

“…The lens fibers originate from the lens‐epithelial cells after their elongation and loss of organelles . The endogenous fluorophores that contribute to the lens fibers' AF are complex and include also unknown species that accumulate with age . As such, a spectral separation of the detected signals was not performed (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…Current applications of TPI regarding imaging of the lens are not extensive, although, it has been used to establish a correlation between the lens‐AF intensity and the progression of presbyopia in a mouse animal model of cataract . The lifetime of the AF of lens fibers was used to evaluate the accumulation of fluorescent compounds with depth . A correlation between diabetes and changes of the lens AF has also been demonstrated using 1‐photon excitation techniques .…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the metabolism and morphology of the porcine cornea, lens and retina by 2‐photon imaging

Batista

Breunig

König

et al. 2018

Journal of Biophotonics

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Two-photon imaging is a noninvasive imaging technique with increasing importance in the biological and medical fields since it allows intratissue cell imaging with high resolution. We demonstrate the feasibility of using a single 2-photon instrument to evaluate the cornea, the crystalline lens and the retina based on their autofluorescence (AF). Image acquisition was performed using a custom-built 2-photon microscope for 5-dimensional microscopy with a near infrared broadband sub-15 femtosecond laser centered at 800 nanometers. Signals were detected using a spectral photomultiplier tube. The spectral ranges for the analysis of each tissue/layer AF were determined based on the spectra of each tissue as well as of pure endogenous fluorophores. The cornea, lens and retina are characterized at multiple depths with subcellular resolution based on their morphology and AF lifetime. Additionally, the AF lifetime of NAD(P)H was used to assess the metabolic activity of the cornea epithelium, endothelium and keratocytes. The feasibility to evaluate the metabolic activity of lens epithelial cells was also demonstrated, which may be used to further investigate the pathogenesis of cataracts. The results illustrate the potential of multimodal multiphoton imaging as a novel ophthalmologic technique as well as its potential as a diagnostic tool.

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“…The mean lifetime of the AF of this layer was (1.42 ±0.10) nanoseconds (Table ). A similar value was reported for superficial cortex fibers of mouse lenses .…”

Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%