Two-photon excited-state dynamics of mEGFP-linker-mScarlet-I crowding biosensor in controlled environments
Sarah A. Mersch,
Sarah Bergman,
Erin D. Sheets
et al.
Abstract:Macromolecular crowding affects many cellular processes such as diffusion, biochemical reaction kinetics, protein-protein interactions, and protein folding. Mapping the heterogeneous, dynamics crowding in living cells or tissues requires genetically encoded,...
Recently, we have investigated the sensitivity of an mEGFP-linker-mScarlet-I construct (GE2.3) in response to macromolecular crowding using ensemble time-resolved two-photon (2P) fluorescence measurements [Mersch et al., PCCP 26 (5), 2024]...
Recently, we have investigated the sensitivity of an mEGFP-linker-mScarlet-I construct (GE2.3) in response to macromolecular crowding using ensemble time-resolved two-photon (2P) fluorescence measurements [Mersch et al., PCCP 26 (5), 2024]...
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