Two-photon fabrication of hydrogel microstructures for excitation and immobilization of cells

Hasselmann, Nils Frederik; Hackmann, Michael J.; Horn, Wolfgang

doi:10.1007/s10544-017-0252-6

Cited by 11 publications

(8 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study again showed the importance of high water content systems to reach good biocompatibility. The above-mentioned work, together with the 2P-microfabrication of waveguides for selective single yeast cells' immobilization and illumination 502 in PEG-DA hydrogel, is, to our knowledge, the only study in which cell-laden synthetic polymers have been used. Importantly, the writing process exhibited high cytotoxicity that can be attributed to the Irgacure 819 which, being an inefficient 2PI, requires higher laser doses.…”

Section: Two-photon Slmentioning

confidence: 99%

Guiding Lights: Tissue Bioprinting Using Photoactivated Materials

et al. 2020

View full text Add to dashboard Cite

Photo-activated materials have found widespread use in biological and medical applications and are playing an increasingly important role in the nascent field of three-dimensional (3D) bioprinting. Light can be used as a trigger to drive the formation or the degradation of chemical bonds, leading to unprecedented spatiotemporal control over a material's chemical, physical, and biological properties. With resolution and construct size ranging from nanometres to centimeters, light-mediated biofabrication allows multicellular and multimaterial approaches. It promises to be a powerful tool to mimic the complex multiscale organization of living tissues including skin, bone, cartilage, muscle, vessels, heart, and liver, among others, with increasing organotypic functionality. With this review, we comprehensively discuss photochemical reactions, photo-activated materials, and their use in state-of-the-art deposition-based (extrusion and droplet) and vat polymerization-based (one-and two-photon) bioprinting. By offering an up-to-date view on these techniques, we identify emerging trends, focusing on both the chemistry and instrument aspects, thereby allowing the readers to select the best-suited approach. Starting with photochemical reactions and photo-activated materials, we then discuss principles, applications, and limitations of each technique. With a critical eye to the most recent achievements, the reader is guided through this exciting, emerging field with special emphasis on cell-laden hydrogel constructs.

show abstract

Section: Two-photon Slmentioning

confidence: 99%

Guiding Lights: Tissue Bioprinting Using Photoactivated Materials

et al. 2020

View full text Add to dashboard Cite

show abstract

“…A second type of approach to cell trapping and sensing/manipulation by means of 3D MPL was given a few years later by Hasselmann et al [ 125 ]. In this study, single fluorescent E. coli cells were trapped by the fast (10–15 s) fabrication of PEGDA hydrogel (with Irgacure TM PIs) pillars (2

) close to the cell.…”

Section: Laser-fabricated Active Microstructured Hydrogelsmentioning

confidence: 99%

“…( B ): Schematic illustration showing the cross-linking process in a cell-loaded hydrogel with a waveguide starting from a fiber connecting a cell for fluorescent excitation. Reprinted from [ 125 ]. ( C ): LSM images of 200 × 200 × 200 μm 3 RCPhC1-NB/SH-based cubes printed in the presence of living ASC–GFPs using 2 mol % DAS with respect to the photo-cross-linkable functionalities (left).…”

Section: Figurementioning

confidence: 99%

“…∼ = 94 fs, λ = 780 nm, f R =80 MHz P = 0.9-2.64 mW ω 0 ∼ = 1 µm, v S =10-30 µm/s E. coli trapping[125,126] PEGda, 700Da) 50 wt%, 2 wt% PI: 2PCK or DAS MPL fabrication τ P ∼ = 150 fs, λ = 800 nm, f R = 80 MHz P = 60-410 mW, v S =1-100 mm/s ∼ = 70 fs, λ = 720 nm, f R = 80 MHz P = 10-100 mW, v S =1000 mm/s ∼ = 100 fs, λ = 810 nm, f R = 73 MHz, P = 400 mW; v S =10 mm/s ∼ = 100 fs, λ = 700-850 nm, f R =80 MHz, P = 0.8 mW; v S =0.8 mm/s fs laser (λ = 780 nm, τ P = 100-200 fs), P = 16 mW, v S =30 µm/s. Sapphire, λ = 780 nm, P = 17 mW, v S =100 µm/s.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Multiphoton Laser Fabrication of Hybrid Photo-Activable Biomaterials

Bouzin

Zeynali

Marini

et al. 2021

Sensors

View full text Add to dashboard Cite

The possibility to shape stimulus-responsive optical polymers, especially hydrogels, by means of laser 3D printing and ablation is fostering a new concept of “smart” micro-devices that can be used for imaging, thermal stimulation, energy transducing and sensing. The composition of these polymeric blends is an essential parameter to tune their properties as actuators and/or sensing platforms and to determine the elasto-mechanical characteristics of the printed hydrogel. In light of the increasing demand for micro-devices for nanomedicine and personalized medicine, interest is growing in the combination of composite and hybrid photo-responsive materials and digital micro-/nano-manufacturing. Existing works have exploited multiphoton laser photo-polymerization to obtain fine 3D microstructures in hydrogels in an additive manufacturing approach or exploited laser ablation of preformed hydrogels to carve 3D cavities. Less often, the two approaches have been combined and active nanomaterials have been embedded in the microstructures. The aim of this review is to give a short overview of the most recent and prominent results in the field of multiphoton laser direct writing of biocompatible hydrogels that embed active nanomaterials not interfering with the writing process and endowing the biocompatible microstructures with physically or chemically activable features such as photothermal activity, chemical swelling and chemical sensing.

show abstract

“…Furthermore, realization of accurate structures becomes more challenging when the structural design has an open geometry, as the capillary forces caused by the evaporating developer lead to significant distortion or structural collapse . To date, most of the PEGDA formulations applied in 2PP exploit low-molar-mass PEGs (<750 g mol –1 ) to obtain an effective microfabrication process owing to a sufficiently high cross-linking density. − ,− Nevertheless, it was reported that the processability of the latter is still prone to deformations associated with swelling and detachment from glass substrates. ,, Moreover, a narrow polymerization window has been observed, which limits tuning of microstructure dimensions and therefore the resolution of the printed structures . Scarpa et al.…”

Section: Introductionmentioning

confidence: 99%

Increasing the Microfabrication Performance of Synthetic Hydrogel Precursors through Molecular Design

et al. 2021

View full text Add to dashboard Cite

Implementation of hydrogel precursors in twophoton polymerization (2PP) technology provides promising opportunities in the tissue engineering field thanks to their soft characteristics and similarity to extracellular matrix. Most of the hydrogels, however, are prone to post-fabrication deformations, leading to a mismatch between the computer-aided design and the printed structure. In the present work, we have developed novel synthetic hydrogel precursors to overcome the limitations associated with 2PP processing of conventional hydrogel precursors such as post-processing deformations and a narrow processing window. The precursors are based on a poly(ethylene glycol) backbone containing urethane linkers and are, on average, functionalized with six acrylate terminal groups (three on each terminal group). As a benchmark material, we exploited a precursor with an identical backbone and urethane linkers, albeit functionalized with two acrylate groups, that were reported as state-of-the-art. An in-depth characterization of the hexafunctional precursors revealed a reduced swelling ratio (<0.7) and higher stiffness (>36 MPa Young's modulus) compared to their difunctional analogs. The superior physical properties of the newly developed hydrogels lead to 2PP-based fabrication of stable microstructures with excellent shape fidelity at laser scanning speeds up to at least 90 mm s −1 , in contrast with the distorted structures of conventional difunctional precursors. The hydrogel films and microscaffolds revealed a good cell interactivity after functionalization of their surface with a gelatin methacrylamide-based coating. The proposed synthesis strategy provides a one-pot and scalable synthesis of hydrogel building blocks that can overcome the current limitations associated with 2PP fabrication of hydrogel microstructures.

show abstract

Two-photon fabrication of hydrogel microstructures for excitation and immobilization of cells

Cited by 11 publications

References 29 publications

Guiding Lights: Tissue Bioprinting Using Photoactivated Materials

Guiding Lights: Tissue Bioprinting Using Photoactivated Materials

Multiphoton Laser Fabrication of Hybrid Photo-Activable Biomaterials

Increasing the Microfabrication Performance of Synthetic Hydrogel Precursors through Molecular Design

Contact Info

Product

Resources

About