Two-Photon Imaging Reveals Somatodendritic Chloride Gradient in Retinal ON-Type Bipolar Cells Expressing the Biosensor Clomeleon

Duebel, Jens; Haverkamp, Silke; Schleich, Wolfram; Feng, Guoping; Augustine, George J; Kuner, Thomas; Euler, Thomas

doi:10.1016/j.neuron.2005.10.035

Cited by 160 publications

(174 citation statements)

References 58 publications

Supporting

Mentioning

162

Contrasting

Unclassified

Order By: Relevance

“…GABA inputs could evoke a dual-effect in rod-BCs: depolarizing the dendrites and hyperpolarizing the axonal terminals (Duebel et al, 2005;Varela et al, 2005a). Interestingly, we also found that KCC2 was present in PKC-positive rod-bipolar dendrites (see Fig.…”

Section: Nkcc1 In the Outer Retinas In Adult Animalssupporting

confidence: 61%

Spatial and temporal distribution patterns of Na-K-2Cl cotransporter in adult and developing mouse retinas

McKERNAN

Shen

2008

Vis Neurosci

View full text Add to dashboard Cite

The Na-K-2Cl cotransporter (NKCC) is a Cl − uptake transporter that is responsible for maintaining a Cl − equilibrium potential positive to the resting potential in neurons. If NKCC is active, GABA and glycine can depolarize neurons. In view of the abundance of GABAergic and glycinergic synapses in retina, we undertook a series of studies using immunocytochemical techniques to determine the distribution of NKCC in retinas of both developing and adult mice. We found NKCC antibody (T4) labeling present in retinas from wild-type mice, but not in NKCC1-deficient mice, suggesting that the NKCC1 subtype is a major Cl − uptake transporter in mouse retina. Strong labeling of NKCC1 was present in horizontal cells and rod-bipolar dendrites in adult mice. Interestingly, we also found that a diffuse labeling pattern was present in photoreceptor terminals. However, NKCC1 was barely detectable in the inner retina of adult mice. Using an antibody against K-Cl cotransporter 2 (KCC2), we found that KCC2, a transporter that extrudes Cl − , was primarily expressed in the inner retina. The expression of NKCC1 in developing mouse retinas was studied from postnatal day (P) 1 to P21, NKCC1 labeling first appeared in the dendrites of horizontal and rod-bipolar cells as early as P7, followed by photoreceptor terminals between P10-P14; with expression gradually increasing concomitantly with the growth of synaptic terminals and dendrites throughout retinal development. In the inner retina, NKCC1 labeling was initially observed in the inner plexiform layer at P1, but labeling diminished after P5. The developmental increase in NKCC expression only occurred in the outer retina. Our results suggest that the distal synapses and synaptogenesis in mouse retinas undergo a unique process with a high intracellular Cl − presence due to NKCC1 expression.

show abstract

Section: Nkcc1 In the Outer Retinas In Adult Animalssupporting

confidence: 61%

Spatial and temporal distribution patterns of Na-K-2Cl cotransporter in adult and developing mouse retinas

McKERNAN

Shen

2008

Vis Neurosci

View full text Add to dashboard Cite

show abstract

“…Although these mice have previously been used for several purposes (Haverkamp et al, 2005;Duebel et al, 2006;Pond et al, 2006), this paper provides the first comprehensive description of these mice and the properties of the transgenically expressed Clomeleon. We also demonstrate that dynamic changes in [Cl − ] i resulting from synaptic activity can be detected in these mice, establishing a new avenue for imaging mechanisms of synaptic inhibition and inhibitory network activity.…”

Section: Discussionmentioning

confidence: 99%

“…Unlike organic Cl − dyes (Verkman, 1990), Clomeleon expression can be targeted to distinct populations of neurons. These properties make Clomeleon suitable for studying many poorly understood aspects of [Cl − ] i regulation, including activity-dependent ionic plasticity (Kaila, 1994;Payne et al, 2003), compartmental gradients of [Cl − ] i (Duebel et al, 2006;Szabadics et al, 2006), sensory signal transduction (Reuter et al, 1998), and pathophysiological mechanisms in a multitude of diseases (Payne et al, 2003;Pond et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon

et al. 2006

Self Cite

View full text Add to dashboard Cite

show abstract

“…Photoreceptors in the surround of the ganglion cell's receptive field may depolarize horizontal cells (Fig. 8Bii), which excite ON bipolar cells (Duebel et al 2006) and finally the ON ganglion cell. This multisynaptic retinal circuitry leads to long-latency spikes (Figs.…”

Section: Characterization Of Capacitive Stimulation Arraymentioning

confidence: 99%

Electrical stimulation of retinal neurons in epiretinal and subretinal configuration using a multicapacitor array

Eickenscheidt

Jenkner

Thewes

et al. 2012

Journal of Neurophysiology

110

120

View full text Add to dashboard Cite

Eickenscheidt M, Jenkner M, Thewes R, Fromherz P, Zeck G. Electrical stimulation of retinal neurons in epiretinal and subretinal configuration using a multicapacitor array. J Neurophysiol 107: 2742-2755, 2012. First published February 22, 2012 doi:10.1152/jn.00909.2011.-Electrical stimulation of retinal neurons offers the possibility of partial restoration of visual function. Challenges in neuroprosthetic applications are the long-term stability of the metal-based devices and the physiological activation of retinal circuitry. In this study, we demonstrate electrical stimulation of different classes of retinal neurons with a multicapacitor array. The array-insulated by an inert oxideallows for safe stimulation with monophasic anodal or cathodal current pulses of low amplitude. Ex vivo rabbit retinas were interfaced in either epiretinal or subretinal configuration to the multicapacitor array. The evoked activity was recorded from ganglion cells that respond to light increments by an extracellular tungsten electrode. First, a monophasic epiretinal cathodal or a subretinal anodal current pulse evokes a complex burst of action potentials in ganglion cells. The first action potential occurs within 1 ms and is attributed to direct stimulation. Within the next milliseconds additional spikes are evoked through bipolar cell or photoreceptor depolarization, as confirmed by pharmacological blockers. Second, monophasic epiretinal anodal or subretinal cathodal currents elicit spikes in ganglion cells by hyperpolarization of photoreceptor terminals. These stimuli mimic the photoreceptor response to light increments. Third, the stimulation symmetry between current polarities (anodal/cathodal) and retina-array configuration (epi/sub) is confirmed in an experiment in which stimuli presented at different positions reveal the center-surround organization of the ganglion cell. A simple biophysical model that relies on voltage changes of cell terminals in the transretinal electric field above the stimulation capacitor explains our results. This study provides a comprehensive guide for efficient stimulation of different retinal neuronal classes with low-amplitude capacitive currents.

show abstract

Two-Photon Imaging Reveals Somatodendritic Chloride Gradient in Retinal ON-Type Bipolar Cells Expressing the Biosensor Clomeleon

Cited by 160 publications

References 58 publications

Spatial and temporal distribution patterns of Na-K-2Cl cotransporter in adult and developing mouse retinas

Spatial and temporal distribution patterns of Na-K-2Cl cotransporter in adult and developing mouse retinas

Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon

Electrical stimulation of retinal neurons in epiretinal and subretinal configuration using a multicapacitor array

Contact Info

Product

Resources

About