Two-photon in vivo imaging of cells

Christensen, Daniel J.; Nedergaard, Maiken

doi:10.1007/s00467-011-1818-9

Cited by 16 publications

(7 citation statements)

References 72 publications

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“…Instead multiphoton microscopy using light with higher wavelengths and allowing a better focus of the illuminating beam is ideal for in vivo imaging (Christensen and Nedergaard, 2011). We next set-up the optimal imaging conditions with a multiphoton microscope LSM 7 MP OPO (Zeiss, France).…”

Section: A Reliable Multiphoton Microscope Set Up For Live Msc Mitochmentioning

confidence: 99%

In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath

et al. 2015

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Section: A Reliable Multiphoton Microscope Set Up For Live Msc Mitochmentioning

confidence: 99%

In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath

et al. 2015

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“…A variety of modalities have been explored for transmitting energy and information through biological media, including: magnetic fields (MRI4 and near-field powered devices5), microwave6, ultrasound78, Infrared9 and photonic10. The exacting requirements of magnetic field strength and uniformity for micro-MRI are well documented, and in general limit the method to the examination of small biological volumes that can be contained within the controlled field environment.…”

Section: Resultsmentioning

confidence: 99%

Electromagnetic limits to radiofrequency (RF) neuronal telemetry

Díaz

Sebastian

2013

Sci Rep

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The viability of a radiofrequency (RF) telemetry channel for reporting individual neuron activity wirelessly from an embedded antenna to an external receiver is determined. Comparing the power at the transmitting antenna required for the desired Channel Capacity, to the maximum power that this antenna can dissipate in the body without altering or damaging surrounding tissue reveals the severe penalty incurred by miniaturization of the antenna. Using both Specific Absorption Rate (SAR) and thermal damage limits as constraints, and 300 Kbps as the required capacity for telemetry streams 100 ms in duration, the model shows that conventional antennas smaller than 0.1 mm could not support human neuronal telemetry to a remote receiver (1 m away.) Reducing the antenna to 10 microns in size to enable the monitoring of single human neuron signals to a receiver at the surface of the head would require operating with a channel capacity of only 0.3 bps.

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“…There is some debate as to which high-resolution imaging method, two-photon or confocal, is the superior choice for fluorescent imaging of cells [1]. Because of the lower wavelengths (400-700 nm) used in confocal imaging, there is a tendency for the tissue to absorb heat and thus it is only suitable for fixed tissue, which can introduce distortions.…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Imaging of the Mouse Organ of Corti Cytoarchitecture for Mechanical Modeling

Puria¹,

Hartman²,

Kim³

et al. 2011

AIP Conference Proceedings

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Abstract. Cochlear models typically use continuous anatomical descriptions and homogenized parameters based on two-dimensional images for describing the organ of Corti. To produce refined models based more closely on the actual cochlear cytoarchitecture, three-dimensional morphometric parameters of key mechanical structures are required. Towards this goal, we developed and compared three different imaging methods: (1) A fixed cochlear whole-mount preparation using the fluorescent dye Cellmask®, which is a molecule taken up by cell membranes and clearly delineates Deiters' cells, outer hair cells, and the phalangeal process, imaged using confocal microscopy; (2) An in situ fixed preparation with hair cells labeled using anti-prestin and supporting structures labeled using phalloidin, imaged using two-photon microscopy; and (3) A membrane-tomato (mT) mouse with fluorescent proteins expressed in all cell membranes, which enables two-photon imaging of an in situ live preparation with excellent visualization of the organ of Corti. Morphometric parameters including lengths, diameters, and angles, were extracted from 3D cellular surface reconstructions of the resulting images. Preliminary results indicate that the length of the phalangeal processes decreases from the first (inner most) to third (outer most) row of outer hair cells, and that their length also likely varies from base to apex and across species.

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Two-photon in vivo imaging of cells

Cited by 16 publications

References 72 publications

In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath

In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath

Electromagnetic limits to radiofrequency (RF) neuronal telemetry

Three-Dimensional Imaging of the Mouse Organ of Corti Cytoarchitecture for Mechanical Modeling

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