Two-photon microscopy at &gt;500 volumes/second

Tsai, Yu Lin; Li, Youfu; Wan, Lin; Wang, Chien-Sheng; Chiang, Chi-Huan; Singh, Vijay Raj; So, Peter T. C.; Chou, Chia-Fu; Chu, Shi‐Wei

doi:10.1101/2020.10.21.349712

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Sofroniew et al, 17 Stirmal et al, 18 Tsai et al, 19 Yu et al, 18 Clough et al 20 Combined 2P calcium imaging and optogenetics ("Alloptical" physiology)…”

Section: Mesoscopic Imagingmentioning

confidence: 99%

Dynamic Fluorescence Imaging in Experimental Epilepsy

Lawlor

Goldberg

2022

Epilepsy Curr

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The ability to develop effective new treatments for epilepsy may depend on improved understanding of seizure pathophysiology, about which many questions remain. Dynamic fluorescence imaging of activity at single-neuron resolution with fluorescent indicators in experimental model systems in vivo has revolutionized basic neuroscience and has the potential to do so for epilepsy research as well. Here, we review salient issues as they pertain to experimental imaging in basic epilepsy research, including commonly used imaging technologies, data processing and analysis, interpretation of results, and selected examples of how imaging-based approaches have revealed new insight into mechanisms of seizures and epilepsy.

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“…Sofroniew et al, 17 Stirmal et al, 18 Tsai et al, 19 Yu et al, 18 Clough et al 20 Combined 2P calcium imaging and optogenetics ("Alloptical" physiology)…”

Section: Mesoscopic Imagingmentioning

confidence: 99%

Dynamic Fluorescence Imaging in Experimental Epilepsy

Lawlor

Goldberg

2022

Epilepsy Curr

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“…Nevertheless, in most cases, 2PM is based on a point scan geometry, thus significantly limiting the imaging speed. Various high speed volumetric 2PM techniques have been developed recently, including light sheet [6], wide-field [7,8], spatio-temporal multiplexing [9][10][11][12], and inertia-free high-speed axial scanning [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Plug-and-play adaptive optics for two photon high-speed volumetric imaging

et al. 2022

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To understand brain functions, it is important to study functional connectivity among stereoscopically distributed neurons. Since the brain is composed of 3D neuron networks, volumetric imaging with high spatiotemporal resolution is highly desirable. Two-photon microscopy (2PM) conveniently offers 3D tissue imaging with sub-micrometer resolution based on its intrinsic optical sectioning and deep penetration capabilities. However, the main challenge lies in the volumetric imaging speed and contrast reduction in deep tissue due to aberration. In this study, we integrate a tunable acoustic gradient (TAG) lens and a plug-and-play adaptive-optics (AO) lens into 2PM. The former provides ~100kHz axial scan rate, achieving volumetric imaging rate in 1-10Hz range, while the latter enhances image contrast by nearly two-fold in deep brain regions via correcting both systematic and sample aberrations. The combination offers a practical approach toward high-speed, high-contrast optical volumetric imaging of brain tissues.

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