Two-Photon Techniques in Tissue Engineering

Schade, R.; Weiß, Thomas; Berg, Albrecht; Schnabelrauch, Matthias; Liefeith, Klaus

doi:10.1177/039139881003300406

Cited by 30 publications

(23 citation statements)

References 22 publications

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“…SU-8), which are not conventional biomaterials. Several of these materials have proven biocompatible and have been used as scaffold materials in cell culture models [12][13][14][15]. A promising new photoresist is SZ2080, which has a hybrid organic-inorganic silicon-zirconium composition [10].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional structural niches engineered via two-photon laser polymerization promote stem cell homing

et al. 2013

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Section: Introductionmentioning

confidence: 99%

Three-dimensional structural niches engineered via two-photon laser polymerization promote stem cell homing

et al. 2013

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“…[61] Any subsequent process such as fluorescence or a photoinduced chemical reaction is thus localized in this small volume enabling a near 100-nm lateral spatial resolution. [62] 2PP has only recently been applied to the microfabrication of 3D scaffolds enabling biocompatibility, [63,64] cell adhesion, [65][66][67][68] and cell migration [69] studies. ECM proteins (like fibrinogen, fibronectin, collagen) as biocompatible scaffold materials were microstructured via singlet oxygen crosslinking using sensitizing dyes [70] even inside living cells.…”

mentioning

confidence: 99%

Two‐Photon Polymerization of Biocompatible Photopolymers for Microstructured 3D Biointerfaces

et al. 2011

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Three‐dimensional microstructured scaffolds provide a means for cells to be cultured in vitro in a way that resembles natural conditions more closely than flat tissue culture polystyrene. In the presented work, two‐photon polymerization (2PP) is applied as a tool for the engineering of high‐resolution 3D scaffold structures with a well defined microarchitecture made of biocompatible photo resins. 2PP is a novel photolithographic technique using femtosecond laser pulses which enables free 3D microstructuring of liquid photo resins due to the relationship of the axial and lateral spatial confinement of the photoreaction to the focal volume of a focused laser beam. A set of photo resins were tested with regard to 2PP processability and three different classes of methacrylated photopolymerizable monomers (methacrylated oligolactones, urethane dimethacrylate, poly(ethylene glycol diacrylate)) were found to be efficient 2PP materials. 3D microstructures based on computer models were produced and tested for biocompatibility. The initial cell adhesion and the viability of bovine chondrocytes on the polymeric scaffolds were evaluated morphologically by confocal laser scanning microscopy (CLSM) after three‐day culture on 2PP derived microstructures. 2PP derived scaffolds were fabricated in different sizes and geometries, starting from the 100 µm‐range reaching out to the cm‐range showing the actual possibilities to produce large volume scaffolds even for implantation purposes.

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“…With high resolution and high fidelity, TPP is capable to fabricate several types of small-scale medical devices, including micro-needles, microfluidic devices, etc. [14,15]. In contrast to other RP techniques, TPP is not restricted to a layer-by-layer build-up of a 3D structure.…”

Section: Two-photon Polymerizationmentioning

confidence: 99%

Selective Laser Sintering and Its Biomedical Applications

Duan

Wang

2013

Laser Technology in Biomimetics

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Selective laser sintering (SLS), a mature and versatile rapid prototyping (RP) technology, uses a laser beam to selectively sinter powdered materials to form three-dimensional objects, porous or non-porous, according to the computer-aided design which can be based on data obtained from advanced medical imaging technologies such as magnetic resonance imaging (MRI) and computer tomography (CT). In this chapter, major RP technologies suitable for biomedical applications are briefly introduced first. A review is made on SLS, including its working principle, modification of commercial SLS machines for fabricating biomedical products, biomedical SLS materials, and optimization of SLS parameters. Finally, a detailed presentation is given on the biomedical application of SLS, focusing on the fabrication of tissue engineering scaffolds and drug or biomolecule delivery vehicles. It is shown that SLS has great potential for many biomimetic and biomedical applications.

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Two-Photon Techniques in Tissue Engineering

Cited by 30 publications

References 22 publications

Three-dimensional structural niches engineered via two-photon laser polymerization promote stem cell homing

Three-dimensional structural niches engineered via two-photon laser polymerization promote stem cell homing

Two‐Photon Polymerization of Biocompatible Photopolymers for Microstructured 3D Biointerfaces

Selective Laser Sintering and Its Biomedical Applications

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