Two plasma membrane H+-ATPase genes are differentially expressed in iron-deficient cucumber plants

Santi, Simonetta; Cesco, Stefano; Varanini, Zeno; Pinton, Roberto

doi:10.1016/j.plaphy.2005.02.007

Cited by 135 publications

(98 citation statements)

References 32 publications

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“…It is well known that Fe-starved roots of dicotyledonous plants change morphologically, showing dense root hair development and typical subapical swellings. In parallel with this change, biochemical changes have been observed, including proton extrusion by the activation of a proton-pump H + -ATPase, thereby reducing soil pH and increasing the solubility of Fe 3+ Waters et al, 2002;Hell and Stephan, 2003;Schmidt, 2003;Santi et al, 2005). Concomitantly, organic acids such as citric and malic acids that act as Fe 3+ -chelators (Ryan et al, 2001;Dakora and Phillips, 2002) are released, chelated Fe 3+ is reduced to Fe 2+ by the action of a membrane-bound Fe 3+ chelate reductase, and then Fe 2+ is transported into cells by a Fe-regulated transporter (Waters et al, 2002;Connolly et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Iron deficiency induces changes in riboflavin secretion and the mitochondrial electron transport chain in hairy roots of Hyoscyamus albus

Higa

Mori

Kitamura

2010

Journal of Plant Physiology

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Authorship note: AH and YM have equal contribution. 3 SummaryHyoscyamus albus hairy roots secrete riboflavin under Fe-deficient conditions. To determine whether this secretion was linked to an enhancement of respiration, both riboflavin secretion and the reduction of 2,3,5-triphenyltetrazolium (TTC), as a measure of respiration activity, were determined in hairy roots cultured under Fe-deficient and Fe-replete conditions, with or without aeration. Appreciable TTC-reducing activity was detected at the root tips, at the bases of lateral roots and in internal tissues, notably the vascular system. TTC-reducing activity increased under Fe deficiency and this increase occurred in concert with riboflavin secretion and was more apparent under aeration. Riboflavin secretion was not apparent under Fe-replete conditions.In order to examine which elements of the mitochondrial electron transport chain (mtETC) might be involved, the effects of the respiratory inhibitors, barbiturate, dicoumarol, malonic acid, antimycin, KCN and salicylhydroxamic acid (SHAM) were investigated. Under Fe-deficient conditions, malonic acid affected neither root growth, TTC-reducing activity nor riboflavin secretion, whereas barbiturate and SHAM inhibited only root growth and TTC-reducing activity, respectively, and the other compounds variously inhibited growth and TTC-reducing activity.Riboflavin secretion was decreased, in concert with TTC-reducing activity, by dicoumarol, antimycin and KCN, but not by SHAM. In Fe-replete roots, all inhibitors which reduced riboflavin secretion in Fe-deficient roots showed somewhat different effects: notably, antimycin and KCN did not significantly inhibit TTC-reducing activity and the inhibition by dicoumarol was much weaker in Fe-replete roots. Combined treatment with KCN and SHAM also revealed that Fe-deficient and Fe-replete roots reduced TTC in different ways. A decrease in the Fe content of mitochondria in Fe-deficient roots was confirmed. Overall, the results suggest that, under conditions of Fe deficiency in H. albus hairy roots, the alternative NAD(P)H dehydrogenases, complex III and complex IV, but not the alternative oxidase, are actively involved both in respiration and in riboflavin secretion.4

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Section: Introductionmentioning

confidence: 99%

Iron deficiency induces changes in riboflavin secretion and the mitochondrial electron transport chain in hairy roots of Hyoscyamus albus

Higa

Mori

Kitamura

2010

Journal of Plant Physiology

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“…Two isoforms of P-ATPases (CsHA1 and CsHA2) were isolated from cucumber (Cucumis sativus L.) and their expressions were analyzed in terms of nutritional status of Fe (Santi et al 2005). The authors showed that in roots with Fe deficiency, there was a greater expression of the CsHA1 gene, which was not detectable in the leaves, and concluded that the Fe supply to the deficient plants caused a decrease in the transcription level of CsHA1.…”

Section: Physiological Basis For Iron Uptake By Plantsmentioning

confidence: 99%

Ecophysiology of iron homeostasis in plants

Krohling

Eutrópio

Bertolazi

et al. 2016

Soil Science and Plant Nutrition

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In nature, iron (Fe) occurs in abundance and ranks fourth among all elements on Earth's surface. Still, its availability to plants is reduced, once this element is in the form of hydrated oxides, which can limit plant productivity and biomass production. On the other hand, in high concentrations, this essential micronutrient for the plants can become a toxic agent, increasing the environmental contamination. Fe is necessary for the maintenance of essential processes like respiration and photosynthesis, participating in the electron transport chain and in the conversion between Fe 2+ and Fe 3+

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“…The gene-specific primers used to amplify: CsFRO1 and 113R1 (5'-TCCTTCATCTCTTTCTGCAACA-3') (Santi at al., 2005). The thermal cycle program was: one initial cycle of 94°C 5 min, followed by cycles of 94°C 30 sec; 56°-60°C 1 min (as annealing temperature we used 56°C for CsFRO1 and 60°C for Cspepc, CsIRT1, CsHA1, and Csactin), 72°C 1 min, with 28 cycles for CsFRO1, CsIRT1, CsHA1, Cspepc and 26 cycles for actin, all followed by a final 72°C elongation cycle for 5 min.…”

Section: Pepcase Assaymentioning

confidence: 99%

“…Concerning to the H + -ATPase, we considered the differential expression level of CsHA1 gene (Santi et al, 2005 (AF071788) (Sanchez & Cejudo, 2003). Cspepc1 showed 79% identity with Atppc3, 74%, with Atppc2 and 78% with Atppc1, while Cspepc2 showed 76% identity with Atppc3, 73% with Atppc2 and 78% with Atppc1.…”

Section: Expression Of Iron Deficiency Response Genes In Cucumber Plantsmentioning

confidence: 99%

“…Enhancement of H + efflux, due to an increase in a P-type H + -ATPase activity in response to iron deprivation, was demonstrated in many Strategy I plants (Schmidt, 1999;Zocchi, 2006). A multigene family encoding different isoforms for H + -ATPase and tissue specific expression patterns have been demonstrated (Palmgren, 2001;Dell'Orto et al, 2002;Santi et al, 2005). After mobilization and reduction, the ferrous form, the unique form to be absorbed by these plants, needs to be taken up across the plasma membrane by a specific iron transporter (IRT1) that has been characterized in A. thaliana (Eide et al, 1996) and successively in pea and tomato (Cohen et al, 1998;Eckhardt et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Modulation of iron responsive gene expression and enzymatic activities in response to changes of the iron nutritional status in Cucumis sativus L.

2010

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Regulation exerted by the iron status of the plant on the iron deficiency responses was investigated in cucumber roots (Cucumis sativus L.) both at the biochemical and molecular level. Absence of iron induced the expression of the CsFRO1, CsIRT1, CsHA1 and the Cspepc1 transcripts that was followed by an increase in the corresponding enzymatic activities. Supply of iron repressed gene expression, in particular those of the Fe(III)-chelate reductase and for the high affinity iron transporter and reduce the enzymatic activities. Our results confirm and extend the hypothesis of a coordinate regulation of these responses. Besides these two activities strictly correlated with iron deficiency adaptation, we considered also the H + -ATPase and the phosphoenolpyruvate carboxylase, that have been shown to be involved in this response.

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Two plasma membrane H+-ATPase genes are differentially expressed in iron-deficient cucumber plants

Cited by 135 publications

References 32 publications

Iron deficiency induces changes in riboflavin secretion and the mitochondrial electron transport chain in hairy roots of Hyoscyamus albus

Iron deficiency induces changes in riboflavin secretion and the mitochondrial electron transport chain in hairy roots of Hyoscyamus albus

Ecophysiology of iron homeostasis in plants

Modulation of iron responsive gene expression and enzymatic activities in response to changes of the iron nutritional status in Cucumis sativus L.

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