Two recessive mutations in FGF5 are associated with the long-hair phenotype in donkeys

Legrand, Romain; Tiret, Laurent; Abitbol, Marie

doi:10.1186/s12711-014-0065-5

Cited by 41 publications

(33 citation statements)

References 24 publications

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“…Hair growth was considered to be regulated by hairless gene, FOXI3 13, hypertrichosis gene, FGF13, Trps1, Sox9 1415, and hair overgrowth gene, ABCA5 16. However, natural long hair, well described angora phenotype, results from a regulator gene, FGF5 , in many kinds of mammals1718192021. Here FGF5 have been included in the candidate genes sets based on low heterozygosity and high genetic differentiation in IMCG population (Fig.…”

Section: Discussionmentioning

confidence: 99%

Scanning of selection signature provides a glimpse into important economic traits in goats (Capra hircus)

Guan

Luo

Tan³

et al. 2016

Sci Rep

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Goats (Capra hircus) are one of the oldest livestock domesticated species, and have been used for their milk, meat, hair and skins over much of the world. Detection of selection footprints in genomic regions can provide potential insights for understanding the genetic mechanism of specific phenotypic traits and better guide in animal breeding. The study presented here has generated 192.747G raw data and identified more than 5.03 million single-nucleotide polymorphisms (SNPs) and 334,151 Indels (insertions and deletions). In addition, we identified 155 and 294 candidate regions harboring 86 and 97 genes based on allele frequency differences in Dazu black goats (DBG) and Inner Mongolia cashmere goats (IMCG), respectively. Populations differentiation reflected by Fst values detected 368 putative selective sweep regions including 164 genes. The top 1% regions of both low heterozygosity and high genetic differentiation contained 239 (135 genes) and 176 (106 genes) candidate regions in DBG and IMCG, respectively. These genes were related to reproductive and productive traits, such as “neurohypophyseal hormone activity” and “adipocytokine signaling pathway”. These findings may be conducive to molecular breeding and the long-term preservation of the valuable genetic resources for this species.

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Section: Discussionmentioning

confidence: 99%

Scanning of selection signature provides a glimpse into important economic traits in goats (Capra hircus)

Guan

Luo

Tan³

et al. 2016

Sci Rep

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“…Further investigation of the wool traits of the targeted founders indicated that GM sheep with loss‐of‐function modifications exhibited an increase in wool staple length, wool stretched length and grease fleece yield. The improvement of wool traits is in agreement with long‐haired phenotypes that were previously observed in spontaneous loss‐of‐function mutations of FGF5 in mice and donkeys , as well as in targeted dysfunctional modification in FGG5 knockout Dorper sheep . Overall, we deduced that the targeted effect most possibly resulted from loss‐of‐function modifications of the FGF5 gene because of a lack of the β‐strand domain of mutant FGF5.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9‐mediated loss of FGF5 function increases wool staple length in sheep

Liu²,

Zhang³

et al. 2017

The FEBS Journal

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Fibroblast growth factor 5 (FGF5) regulates hair length in humans and a variety of other animals. To investigate whether FGF5 has similar effects in sheep, we used clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) to generate loss-of-function mutations with the FGF5 gene in Chinese Merino sheep. A total of 16 lambs were identified with genetic mutations within the targeting locus: 13 lambs had biallelic modifications and three lambs had monoallelic modifications. Characterization of the modifications revealed that 13 were frameshift mutations that led to premature termination, whereas the other three were inframe deletions. Thus, CRISPR/Cas9 efficiently generated loss-of-function mutations in the sheep FGF5 gene. We then investigated the effect of loss of FGF5 function on wool traits in 12 lambs and found that wool staple length and stretched length of genetically modified (GM) yearling sheep were significantly longer compared with that of wild-type (WT) control animals. The greasy fleece weight of GM yearling sheep was also significantly greater compared with that of WT sheep. Moreover, the mean fiber diameter in GM sheep showed no significant difference compared with WT sheep, suggesting that the increase in greasy fleece weight was likely attributed to the increase in wool length. The results of this study suggest that CRISPR/Cas9-mediated loss of FGF5 activity could promote wool growth and, consequently, increase wool length and yield.

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“…Studies on Przewalski horses (Equus przewalskii) (Yasinetskaja 1999) and Polish Konik horses (Stachurska et al 2007) showed that the coat cover hairs are thinner in winter than in summer. This phenomenon may be due to hormone changes induced by day length increase in the spring, causing hair follicles to enter catagen (regressive phase of the hair cycle) and telogen (dormancy) (Nixon et al 2002, Legrand et al 2014). On the other hand, in the summer, nutrition is richer and more differentiated than in winter, which can increase the horse hair thickness.…”

Section: Introductionmentioning

confidence: 99%

Coat cover hair density is a symptom of primitive horse adaptation to the environment

Stachurska¹,

Pluta²,

Wójcik³

et al. 2018

PHK

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The objective of the study was to determine whether the coat cover in Polish Konik mares is more dense in winter than in summer and whether the possible difference is effected by the system of the horses' maintenance, lactation or age. Thirty three Polish Konik horses were included: 23 mares kept in a stable and pasture/paddock, and 10 mares living on a close reserve. The study was conducted in July and December, hence summer and winter coats were analysed. The measurements were taken from single horses. Nineteen mares were examined in both seasons, whereas five mares were studied only in the summer and other nine mares only in the winter which resulted in 52 measurements in total. The mares were split into four age groups. Most of mares in both groups lactated in summer, whereas in winter only reserve mares had foals at foot. The coat hair density was determined from the horse's scapula, where an approximately 9 cm 2 area was shaved to obtain hair 0.5 mm in length. Photographs of this area were taken and after conversion a 1 mm 2 square net was put on the picture to enable counting the hair leavings. The measurement was the number of hairs in 1 cm 2 . Data were analyzed using three-way ANOVA with repeated measures (summer, winter), considering two factors: the system of maintenance (stable, reserve) and lactation (lactating mares, non-lactating mares). The age group factor was considered in an additional two-way ANOVA with repeated measures. Spearman's correlation, Pearson's correlations, regression and determination coefficients were determined. The results showed that the coat hair density is a symptom of primitive horses' adaptation to changing environmental conditions. The hair density is significantly higher in winter than in summer, and the density in summer and winter is correlated. The coat hair is denser in reserve mares that are subject to more severe conditions than in stable mares. In winter, the coat hair density increases with the mares' age, which may be associated with higher requirements for isolation from harsh conditions in older mares. The coat hair density is not related to the mare's lactation. These findings may be important for improving the welfare of stable horses.Citation: Stachurska A., Pluta M., Wójcik M., Giżejewski Z., Janczarek I., Różańska-Boczula M. (2018) Coat cover hair density is a symptom of primitive horse adaptation to the environment. Pferdeheilkunde 34, 550-556;Coat cover hair density is a symptom of primitive horse adaptation to the environment A. Stachurska et al.

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Two recessive mutations in FGF5 are associated with the long-hair phenotype in donkeys

Cited by 41 publications

References 24 publications

Scanning of selection signature provides a glimpse into important economic traits in goats (Capra hircus)

Scanning of selection signature provides a glimpse into important economic traits in goats (Capra hircus)

CRISPR/Cas9‐mediated loss of FGF5 function increases wool staple length in sheep

Coat cover hair density is a symptom of primitive horse adaptation to the environment

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