Two related viral genes are located on a single superhelical DNA segment of the multipartite Campoletis sonorensis virus genome

Blissard, Gary W.; Smith, O. P.; Summers, Max D.

doi:10.1016/0042-6822(87)90052-3

Cited by 46 publications

(56 citation statements)

References 29 publications

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“…Some sequences in the 3hUTR may also affect the poly(A) stability (Peltz et al, 1991). In our Blissard et al, 1987). The whole regions show 63 % nucleotide identity, while the sequences between k376 and j6 of WHv1.0 promoter and between k433 and j6 of WHv1.6 promoter show 75 % identity.…”

Section: Discussionmentioning

confidence: 81%

“…Very early expression of the cysteine-rich CsPDV genes resembles that of the baculovirus immediate early genes. All the CsPDV cysteine-rich genes (WHv1.6, WHv1.0, VHv1.4 and VHv1.1) have TATA box motifs 25-35 nucleotides upstream of the transcription initiation site (TIS) (Blissard et al, 1987 ;Dib-Hajj et al, 1993 ;Cui & Webb, 1996). Preliminary gel retardation assays show binding of nuclear protein(s) to the region containing the TATA box (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…1 a, 3h) containing the WHv1.6 gene polyadenylation signals was then cloned downstream of the CAT ORF. This fragment contains 150 bp of the 3h untranslated region (UTR) of the WHv1.6 gene (Blissard et al, 1987). To evaluate the region upstream of the PstI site in the promoter, a 1n5 kb XhoI-PstI fragment in segment W (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…A 639 bp fragment ( Fig. 1 a, 5ha) upstream of the ATG codon of WHv1.6 gene was amplified in a GeneAmp 480 (Perkin Elmer Cetus) with T7 and gene-specific primers (5h GAATTC-GGATCCGATCTCAGCAATGCTATG 3h) (BamHI site underlined) using a cloned PstI (11.45)-HindIII (13.29) fragment of segment W as the template (see Blissard et al, 1987, for physical map of segment W). The following cycles were used for the reaction : 1n5 min at 94 mC, 1n5 min at 56 mC, 3 min at 72 mC, 35 cycles.…”

Section: Methodsmentioning

confidence: 99%

“…Because of their importance in regulating host physiology, most studies have emphasized the isolation of PDV genes that are expressed in lepidopteran hosts. Five class II viral genes have been isolated, WHv1.6, 1.0, VHv1.1, 1.4 and BHv0.9 (Blissard et al, 1987 ;Theilmann & Summers, 1987 ;Dib-Hajj et al, 1993 ;Cui & Webb, 1996), which belong to two distinct gene families (Summers & Dib-Hajj, 1995). Four of these class II genes (WHv1.0, 1.6, VHv1.1, 1.4) have various degrees of identity to each other and constitute a cysteine-rich PDV gene family (Dib-Hajj et al, 1993 ;Cui & Webb, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Promoter analysis of a cysteine-rich Campoletis sonorensis polydnavirus gene.

Cuit

Webb

1997

Journal of General Virology

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Promoter analysis of a cysteine-rich Campoletis sonorensis polydnavirus gene.

Cuit

Webb

1997

Journal of General Virology

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Expression and hemocyte-targeting of aCampoletis sonorensis polydnavirus cysteine-rich gene inHeliothis virescens larvae

Cui

Soldevila

Webb

1997

Arch. Insect Biochem. Physiol.

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The polydnavirus associated with the parasitic wasp Campoletis sonorensis is injected into the lepidopteran insect, Heliothis virescens, during parasitization, after which viral gene products suppress the cellular immune system of the hosts. Four related cysteine‐rich polydnavirus genes have been identified in parasitized H. virescens larvae and grouped into a family. In this study, we investigated the expression and hemocyte targeting of the cysteine‐rich Vhv1.4 protein. Full‐ length and truncated Vhv1.4 proteins were produced in a bacterial expression system, and the purified proteins were used to raise polyclonal antisera. In immunoblots the Vhv1.4 protein was detected in parasitized insects as early as 6 h and throughout the entire course of parasitism. The Vhv1.4 protein appeared predominantly in the plasma fraction of hemolymph from parasitized larvae, suggesting that this protein is secreted. The Vhv1.4 protein expressed from a recombinant baculovirus was secreted in two lepidopteran cell lines and in larvae injected with the recombinant virus. Digestion with endoglycosidases suggests that the Vhv1.4 protein is glycosylated at multiple N‐glycosylation sites. Immunofluorescence assays showed that the Vhv1.4 protein binds to the hemocytes, most notably the granulocytes, in H. virescens larvae. After binding, the Vhv1.4 protein was internalized, probably by endocytosis. Specific binding of the Vhv1.4 to granulocytes implies an important function in the suppression of host cellular encapsulation response. Arch. Insect Biochem. Physiol. 36:251–271, 1997. © 1997 Wiley‐Liss, Inc.

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Identification of host translation inhibitory factor ofCampoletis sonorensis ichnovirus on the tobacco budworm,Heliothis virescens

Kim

2005

Arch. Insect Biochem. Physiol.

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Parasitization of a wasp, Campoletis sonorensis, against the larvae of Heliothis virescens depresses synthesis of specific host proteins related to growth and immunity. It has been suggested that the inhibition of host gene expression is targeted at a posttranscriptional level. This study aimed to verify the identity of host translation inhibitory factor (HTIF) derived from wasp parasitization. To identify HTIF, the proteins in the parasitized host were fractionated using different protein purification methods, and each fraction's HTIF activity was assessed. In the course of the protein purification steps, HTIF activity was highly correlated with the fractions containing VHv 1.4 protein, which has a conserved cysteine-motif and is encoded in C. sonorensis ichnovirus (CsIV). Purified VHv 1.4 protein using an immunoaffinity column exhibited a significant HTIF effect, while the heat-inactivated VHv 1.4 did not. Both recombinant VHv 1.4 and VHv 1.1 (another cys-motif protein encoded in CsIV) proteins were synthesized in Sf 9 cells through a baculovirus expression system. The purified recombinant VHv 1.4 and VHv 1.1 exhibited significant HTIF activities in a nanomolar range. However, VHv1.4 protein showed about four times higher HTIF activity than did VHv 1.1 protein. Both HTIFs acted directly on translation machinery because they inhibited a cell-free in vitro translation system using rabbit reticulocyte lysate. Both HTIFs are likely to discriminate specific target mRNAs because they inhibited translation of RNA extracts from the Tn 368 cell line, but not from Sf 9 cells. In addition, they inhibited translation of RNAs from fat body, hemocytes, and testis, but not from epidermis, gut, labial gland, and nerve tissues of H. virescens. These results indicate that both cys-motif proteins of VHv 1.4 and VHv 1.1 play a role as HTIF in C. sonorensis parasitization.

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Two related viral genes are located on a single superhelical DNA segment of the multipartite Campoletis sonorensis virus genome

Cited by 46 publications

References 29 publications

Promoter analysis of a cysteine-rich Campoletis sonorensis polydnavirus gene.

Promoter analysis of a cysteine-rich Campoletis sonorensis polydnavirus gene.

Expression and hemocyte-targeting of aCampoletis sonorensis polydnavirus cysteine-rich gene inHeliothis virescens larvae

Identification of host translation inhibitory factor ofCampoletis sonorensis ichnovirus on the tobacco budworm,Heliothis virescens

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