Two relatively distinct patterns of ameloblastoma: an anti‐apoptotic proliferating site in the outer layer (periphery) and a pro‐apoptotic differentiating site in the inner layer (centre)

Sandra, Ferry; Nakamura, Norifumi; Mitsuyasu, Takeshi; Shiratsuchi, Yuji; Ohishi, Masamichi

doi:10.1046/j.1365-2559.2001.01138.x

Cited by 65 publications

(82 citation statements)

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“…Since KOT had been classified as an odontogenic cyst, several authors have compared cell proliferation between KOT and odontogenic cysts (8,12,13). Although several authors have assessed cell proliferation and apoptosis in the odontogenic epithelium in AM and in the epithelial lining in KOT (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22), few studies have compared cell proliferation between these tumors (7,8,11) and no previous study has simultaneously evaluated cell proliferation and apoptotic indexes in AM and KOT, comparing both lesions. Therefore, this is the first study assessing comparatively cell proliferation and apoptosis indexes in AM and KOT, although tissue cell population is controlled by a balance among these processes.…”

Section: Discussionmentioning

confidence: 99%

“…This antigen is considered to be a better specific marker of proliferating cells (23). Although several authors have used Ki-67 to access cell proliferation in KOT (12,13) and AM (19,20), few studies have compared cell proliferation between AM and KOT using the Ki-67 (10).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have evaluated apoptosis and related apoptotic factors in the odontogenic epithelium of AM and epithelial lining of KOT (10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Nevertheless, despite the fact that the cell population is controlled by the balance between cell proliferation and apoptosis, no study has evaluated simultaneously apoptotic and proliferation indexes in AMs and KOTs.…”

Section: Introductionmentioning

confidence: 99%

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Cell proliferation and apoptosis in ameloblastomas and keratocystic odontogenic tumors

et al. 2012

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Braz Dent J 23(2) 2012Proliferation and apoptosis in odontogenic tumors 91 INTRODUCTIONAmeloblastoma (AM) is a locally invasive benign epithelial odontogenic tumor that may arise from rests of dental lamina, enamel organ rests, cell rests, the epithelial lining of an odontogenic cyst or from the basal cell layer of oral mucosa (1). Its occurrence varies from 10% to 45.2% of all odontogenic tumors and reaches approximately 1% of all oral cavity neoplasms (2). The usual clinical presentation includes a painless swelling with expansion of the jaws. It can be classified in 3 main groups: solid or multicystic ameloblastoma, unicystic and peripheral. Among these, the solid type is more common and histopathologically represented by follicular, plexiform, acanthomatous, desmoplastic, granular and basal cell patterns (3). A high proliferative activity of the odontogenic epithelium in ameloblastoma (AM) and keratocystic odontogenic tumor (KOT) has been demonstrated. However, no previous study has simultaneously evaluated cell proliferation and apoptotic indexes in AM and KOT, comparing both lesions. The aim of this study was to assess and compare cell proliferation and apoptotic rates between these two tumors. Specimens of 11 solid AM and 11 sporadic KOT were evaluated. The proliferation index (PI) was assessed by immunohistochemical detection of Ki-67 and the apoptotic index (AI) by methyl green-pyronine and in situ DNA nick end-labelling methods. KOT presented a higher PI than AM (p<0.05). No statistically significant difference was found in the AI between AM and KOT. PI and AI were higher in the peripheral cells of AM and respectively in the suprabasal and superficial layers of KOT. In conclusion, KOT showed a higher cell proliferation than AM and the AI was similar between these tumors. These findings reinforce the classification of KOT as an odontogenic tumor and should contribute to its aggressive clinical behavior. Cell Proliferation and Apoptosis in Ameloblastomas and Keratocystic Odontogenic Tumors

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cell proliferation and apoptosis in ameloblastomas and keratocystic odontogenic tumors

et al. 2012

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“…This results suggested that these epithelial cyst linings were neither simple nor neoplastic, but comparatively differentiated stratified squamous epithelia. The bcl-2 proto-oncogene is a member of a gene family in the suppressors of the programmed cell death (17,(27)(28)(29). Recent studies have suggested that there is a close relationship between bcl-2 protein expression and cell population which are long lived, and/or with high proliferation ability (3,29).…”

Section: Discussionmentioning

confidence: 99%

“…The bcl-2 proto-oncogene is a member of a gene family in the suppressors of the programmed cell death (17,(27)(28)(29). Recent studies have suggested that there is a close relationship between bcl-2 protein expression and cell population which are long lived, and/or with high proliferation ability (3,29). Immunoreactivity for bcl-2 protein has been detected in the enamel organs and the dental lamina of tooth germs as well as in most neoplastic cells of ameloblastoma (3,30).…”

Section: Discussionmentioning

confidence: 99%

Odontogenic Keratocyst, Orthokeratinized Odontogenic Cyst and Epidermal Cyst: An Immunohistochemical Study Including Markers of Proliferation, Cytokeratin and Apoptosis Related Factors

Koizumi

2004

Int J Oral-Med Sci

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Objective: There are microscopic similarities among odontogenic keratocyst (OKC), orthokeratinized odontogenic keratocyst (DOC) and epidermal cyst (EDC). This study was to compare with cell proliferation, cell differentiation and apoptosis related factors in OKC, DOC and EDC in order to clarify the characters and category of three hitstopathologically similar cysts.Material and Methods: Fourteen cases of OKC, nine cases of DOC and eighteen cases ofEDC were examined immunohistochemically using antibodies against cytokeratins (CK7,10, 10/13,17,18,19,20) and apoptosis related factors (bcl-2 and Bax).Results: Ki-67 labeling index in OKC was statistically higher than that in DOC and EDC (p<0.05). And that in DOC was higher in EDC (p<0.05). In OKC, CKs and bcl-2 expression patterns were different from DOC and EDC, while both DOC and EDC showed similar reactivity with CKs and bcl-2.Conclusions: The present results suggested that OKC had high cellular proliferation and was relatively low differentiation as compare with DOC and EDC. In addition, there was participation of apoptosis suppressor in OKC. The character of DOC occupied an intermediate position between OKC and EDC. Therefore, it was possible to consider DOC as a distinct entity from OKC. EDC had the lowest cellular activity in the three cysts and it was comparatively maturated lesion.

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Expression of survivin and X chromosome-linked inhibitor of apoptosis protein in ameloblastomas

Kumamoto

Ooya

2004

Virchows Archiv

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To clarify the role of apoptosis in oncogenesis and cytodifferentiation of odontogenic epithelium, expression of survivin and X chromosome-linked inhibitor of apoptosis protein (XIAP), inhibitor of apoptosis protein (IAP) family proteins, was examined in tooth germs and in benign and malignant ameloblastomas by means of immunohistochemistry and reverse-transcription polymerase chain reaction. Immunoreactivity for survivin and XIAP was detected in developing and neoplastic odontogenic epithelium. In tooth germs, survivin expression was evident in inner enamel epithelium. Follicular, plexiform and metastasizing ameloblastomas showed survivin reactivity chiefly in neoplastic cells neighboring the basement membrane, and most neoplastic cells in basal cell and desmoplastic ameloblastomas and ameloblastic carcinomas were positive for survivin. Survivin mRNA levels were slightly higher in ameloblastomas than in tooth germs, suggesting that elevation of survivin expression might be involved in oncogenesis of odontogenic epithelium. Immunoreactivity for XIAP was detected in most odontogenic epithelial cells in tooth germs and in benign and malignant ameloblastomas, and XIAP mRNA levels were significantly higher in follicular ameloblastomas than in plexiform ameloblastomas. The expression of survivin and XIAP in odontogenic tissues suggests that these IAP family proteins contribute to the biological properties of ameloblastomas, such as cell survival, proliferation, differentiation and tissue structuring, as well as to cellular regulation during tooth development.

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Two relatively distinct patterns of ameloblastoma: an anti‐apoptotic proliferating site in the outer layer (periphery) and a pro‐apoptotic differentiating site in the inner layer (centre)

Cited by 65 publications

References 18 publications

Cell proliferation and apoptosis in ameloblastomas and keratocystic odontogenic tumors

Cell proliferation and apoptosis in ameloblastomas and keratocystic odontogenic tumors

Odontogenic Keratocyst, Orthokeratinized Odontogenic Cyst and Epidermal Cyst: An Immunohistochemical Study Including Markers of Proliferation, Cytokeratin and Apoptosis Related Factors

Expression of survivin and X chromosome-linked inhibitor of apoptosis protein in ameloblastomas

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