Two replication-defective adenoviral vaccine vectors for the induction of immune responses to PPRV

Rojas, José M.; Moreno, Hector; García, A. J.; Ramírez, Juan Camilo; Sevilla, Noemı́; Martı́n, Verónica

doi:10.1016/j.vaccine.2013.11.033

Cited by 19 publications

(30 citation statements)

References 51 publications

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“…However, no protection assays were performed in either vaccination study. Similarly, our group reported the potential use as a vaccine for PPRV of the two recombinant replication defective human Ad5 adenoviruses expressing the F or H proteins from PPRV [25], that induced specific humoral and cellular responses to PPRV in mice. In this report, we have extended our previous analyses to sheep, showing that both Ad5-PPRV-F or Ad5-PPRV-H induce humoral and cellular responses in inoculated sheep.…”

Section: Discussionmentioning

confidence: 83%

“…Previously, we have generated two recombinant adenoviruses expressing the F or H proteins from PPRV and demonstrated that they induce specific PPRV neutralizing antibodies as well as cellular immune responses to PPRV in mice [25]. In the present study, protective immune response to these two recombinant adenoviruses was evaluated in sheep.…”

Section: Introductionmentioning

confidence: 87%

“…PPRV strains Nigeria 75/1 or Ivory Coast'89 (ICV) (lineages I and II), respectively, were obtained from Dr. Batten, IAH Pirbright, and recombinant adenoviruses (Ad5-PPRV-F and Ad5-PPRV-H) were used as detailed in [25].…”

Section: Methodsmentioning

confidence: 99%

“…Anti-PPRV IgG ELISA were adapted from a method previously described [25]. Briefly, ELISA plates (Maxisorp, Nunc) were coated with purified PPRV Nigeria 75/1 overnight at 4°C (equivalent to 10 4 pfu per well).…”

Section: Methodsmentioning

confidence: 99%

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Vaccination with Recombinant Adenoviruses Expressing the Peste des Petits Ruminants Virus F or H Proteins Overcomes Viral Immunosuppression and Induces Protective Immunity against PPRV Challenge in Sheep

et al. 2014

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Peste des petits ruminants (PPR) is a highly contagious disease of small ruminants caused by the Morbillivirus peste des petits ruminants virus (PPRV). Two recombinant replication-defective human adenoviruses serotype 5 (Ad5) expressing either the highly immunogenic fusion protein (F) or hemagglutinin protein (H) from PPRV were used to vaccinate sheep by intramuscular inoculation. Both recombinant adenovirus vaccines elicited PPRV-specific B- and T-cell responses. Thus, neutralizing antibodies were detected in sera from immunized sheep. In addition, we detected a significant antigen specific T-cell response in vaccinated sheep against two different PPRV strains, indicating that the vaccine induced heterologous T cell responses. Importantly, no clinical signs and undetectable virus shedding were observed after virulent PPRV challenge in vaccinated sheep. These vaccines also overcame the T cell immunosuppression induced by PPRV in control animals. The results indicate that these adenovirus constructs could be a promising alternative to current vaccine strategies for the development of PPRV DIVA vaccines.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Vaccination with Recombinant Adenoviruses Expressing the Peste des Petits Ruminants Virus F or H Proteins Overcomes Viral Immunosuppression and Induces Protective Immunity against PPRV Challenge in Sheep

et al. 2014

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“…Developing genetically marked vaccines would be advantageous during the eradication phase. Subunit vaccines prepared by expressing the PPRV F and/or H gene in pox and adeno viral vectors found to be experimentally protective (Caufour et al, 2014;Chandran et al, 2010;Chen et al, 2010;Rojas et al, 2014). Attempt was made to develop a subunit vaccine by expressing the H protein of PPRV in peanut plants (Arachis hypogea) and found to elicit neutralizing antibody responses in sheep (Khandelwal et al, 2011).…”

Section: Vaccinementioning

confidence: 99%