Two Small Lymphocyte Subpopulations in Human Peripheral Blood

Hokland, Peter; Hokland, Marianne; Heron, Iver

doi:10.1159/000232146

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…Apart from such complement re ceptors, B lymphocytes also carry internally produced immunoglobulins on their surface. These are usually detected by fluorescent heteroantisera [24], but they can also be de tected through a more sensitive rosette test with autologous erythrocytes coupled (by chromic chloride) with similar heteroantis era [8,12], Sensitization of erythrocytes with IgG antibodies (EA complexes) can be used for determinations of Fc-IgG receptor-carrying lymphocytes. These include subpopulations of both B [5] and T lymphocytes [21] and the major part of the so-called third subpo pulation [7],…”

Section: Introductionmentioning

confidence: 99%

Effects of Basic Proteins on Spontaneous Rosette Formation between Human Lymphocytes and Xenogeneic Erythrocytes

Hokland¹,

Heron²,

Larsen

1981

Int Arch Allergy Immunol

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Two basic proteins (methylated human serum albumin, MHSA, and protamine sulphate, PS) were investigated for their modulatory capacities on a panel of human lymphocyte surface markers. MHSA and PS enhanced the spontaneous rosettes with mouse, sheep and horse erythrocytes, but did not affect the number of rosettes with zymosan particles coated with human complement, or with sheep erythrocytes sensitized with rabbit anti-sheep IgG antibody. Whereas the standard sheep (E) rosette test was not influenced by the negatively charged glycoprotein ceruloplasmin, this compound could, however, abolish the MHSA- and PS-induced enhancements of the number of sheep rosettes. By double marker studies it was furthermore demonstrated that a subset of B cells was induced by MHSA to form E rosettes, and that the same agent increased the number of B cells forming mouse rosettes, so that nearly all B cells were made positive for this marker.

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Section: Introductionmentioning

confidence: 99%

Effects of Basic Proteins on Spontaneous Rosette Formation between Human Lymphocytes and Xenogeneic Erythrocytes

Hokland¹,

Heron²,

Larsen

1981

Int Arch Allergy Immunol

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Effector Cell Potential of Two Human T‐Cell Subpopulations with Different Affinities to Sheep Erythrocytes

Hokland¹,

Heron²

1980

Scand J Immunol

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Two human T lymphocyte subpopulations were purified on the basis of their different affinities to sheep erythrocytes and compared with unseparated and non-T lymphocytes as effectors in cell-mediated lympholysis (CML) after mixed lymphocyte culture (MCL), in antibody-dependent cellular cytotoxicity (ADCC) against the mouse mastocytoma line P 815, and in spontaneous cell-mediated cytotoxicity (SCMC) against the human myeloid leukaemia line K 562 and the Burkitt lymphoma line RAJI. The low-avidity T cells (T1) had developed into more potent effectors than the high-avidity (Te) when assessed in CML. In ADCC and SCMC both subsets exhibited low but consistent lysis with no demonstrable differences. Addition of human leucocyte interferon to the ADCC and SCMC cultures resulted in enhanced SCMC, most notable and to an equal extent with the T cell subsets. In contrast, ADCC of unseparated cells, non-T cells, and the two T subsets was found to be unchanged after interferon addition. These data are discussed in relation to findings with human T-cell subpopulations purified by other methods.

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Mitogenic Activation of Human Lymphocytes: a Protein A Plaque Assay Evaluation of Polyclonal B‐Cell Activators

Hammarström¹,

Bird²,

Smith³

1980

Scand J Immunol

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Using the protein A plaque assay, the capacity of various polyclonal B cell activators to induce differentiation in human B lymphocytes was investigated. Dextran sulphate and native dextran were both virtually devoid of mitogenic properties. Lipopolysaccharide, however, was found to be a potent mitogen in human cells that, although giving rise to low DNA synthetic response, induced high numbers of immunoglobulin-synthesizing cells. Mean plaque-forming cell (PFC) numbers in healthy blood donors assayed on the optimal day (days 5-7) were 23,493 IgM/10(6) cells, 11,288 IgG/10(6) cells, and 2643 IgA/10(6) cells. Values obtained in spleen cells, peaking at days 4-6, were slightly higher. Purified protein derivative (PPD) was equally or even more effective than lipopolysaccharide (LPS) in generating PFC of different subclasses in peripheral blood with mean of 29,241 IgM/10(6), 21,269 IgG/10(6), and 3681 IgA/10(6). PPD furthermore induced a marked DNA synthetic response in human lymphocytes. These data suggest that LPS and PPD may both be used as functional markers in human cells when analysing patients with a suspected immunodeficiency state. It is suggested that cultures should be assayed using the protein A plaque assay, thereby being able not only to investigate the individual immunoglobulin classes but also to avoid the possible hazards involved in measuring antigen-specific responses in patients whose prior immunization to the antigen tested can never be totally excluded.

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Two Small Lymphocyte Subpopulations in Human Peripheral Blood

Cited by 3 publications

References 8 publications

Effects of Basic Proteins on Spontaneous Rosette Formation between Human Lymphocytes and Xenogeneic Erythrocytes

Effects of Basic Proteins on Spontaneous Rosette Formation between Human Lymphocytes and Xenogeneic Erythrocytes

Effector Cell Potential of Two Human T‐Cell Subpopulations with Different Affinities to Sheep Erythrocytes

Mitogenic Activation of Human Lymphocytes: a Protein A Plaque Assay Evaluation of Polyclonal B‐Cell Activators

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