Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.

Lerner, Michael R.; Andrews, Nancy C.; Miller, George; Steitz, Joan A.

doi:10.1073/pnas.78.2.805

Cited by 521 publications

(320 citation statements)

References 26 publications

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“…Furthermore, CR2 serves as the B cell receptor for EBV (2&23), and autoantibodies to a complex of protein and EBV-encoded RNA have been shown to occur in SLE (46), as has impaired T cell regulation of EBVinduced polyclonal B cell proliferation (47).…”

Section: Discussionmentioning

confidence: 99%

Decreased expression of the C3b/C4b receptor (CR1) and the C3d receptor (CR2) on B lymphocytes and of CR1 on neutrophils of patients with systemic lupus erythematosus

Wilson

Ratnoff

Schur

et al. 1986

Arthritis & Rheumatism

167

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Decreased numbers of complement receptor type 1 (CR1) have been observed on erythrocytes of patients wilh systemic lupus erythematosus (SLE) and on glomerular podocytes of patients having proliferative nephritis of SLE. In the present study, the analysis of the cellular expression of CR1 has been extended to include leukocytes. In addition, expression by B lymphocytes of the C3d receptor (CR2), which also serves as the receptor for the Epstein-Barr virus, was assessed. Receptor expression was measured by 2-color fluorescent flow cytometry of peripheral blood B cells, identified by the presence of the B1 antigen, that had also been stained with anti-CR1 or anti-CR2. B cells from 17 patients with SLE exhibited a mean relative fluorescence for CR1 that was 61% of that found in 17 normal individuals (P < 0.001). The expression of CR2 by the patients' B cells (n = 14) was 62% of that of the B cells fraim normal subjects (n = 17) (P < 0.001). The expression of CR1 correlated with that of CR2 among patients (r = 0.63; P < 0.01) but not with the expression of CR2 among normal individuals (r = 0.36; P > 0.1).Thle mean CR1 content of the patients' neutrophils was only 59% of the normal mean (P < 0.001). Thus, abnormalities of complement receptor expression occur

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Section: Discussionmentioning

confidence: 99%

Decreased expression of the C3b/C4b receptor (CR1) and the C3d receptor (CR2) on B lymphocytes and of CR1 on neutrophils of patients with systemic lupus erythematosus

Wilson

Ratnoff

Schur

et al. 1986

Arthritis & Rheumatism

167

View full text Add to dashboard Cite

show abstract

“…EBER1 has been previously demonstrated to bind to PKR and has been suggested to confer resistance to Fas-mediated apoptosis in tissue culture cells by blocking PKR activity (Clarke et al 1991;Sharp et al 1993;Nanbo et al 2005). EBER1 and EBER2 are the most abundant viral transcripts expressed during viral latency (∼5 × 10 6 per cell) and are predominantly localized in the nucleus (Lerner et al 1981;Howe and Steitz 1986;Fok et al 2006). The EBER RNAs were also found to play a key role in the maintenance of the malignant phenotypes of Burkitt's lymphoma cells (Nanbo and Takada 2002).…”

Section: Eber Rnasmentioning

confidence: 98%

Eukaryotic regulatory RNAs: an answer to the ‘genome complexity’ conundrum

Prasanth¹,

Spector²

2007

Genes Dev.

365

305

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A large portion of the eukaryotic genome is transcribed as noncoding RNAs (ncRNAs). While once thought of primarily as "junk," recent studies indicate that a large number of these RNAs play central roles in regulating gene expression at multiple levels. The increasing diversity of ncRNAs identified in the eukaryotic genome suggests a critical nexus between the regulatory potential of ncRNAs and the complexity of genome organization. We provide an overview of recent advances in the identification and function of eukaryotic ncRNAs and the roles played by these RNAs in chromatin organization, gene expression, and disease etiology.

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“…In the nucleus, it associates with the U-rich 3Ј termini of newly synthesized RNA polymerase III transcripts, facilitates transcriptional termination and transcript release from the DNA (18,19,33), and even promotes the initiation of polymerase III transcription (32). It also binds to small RNAs in some virus-infected cells (28,48,70). Three cytoplasmic functions have been described for La.…”

Section: Discussionmentioning

confidence: 99%

Human La Protein: a Stabilizer of Histone mRNA

McLaren

Caruccio

Ross

1997

Molecular and Cellular Biology

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Histone mRNA is destabilized at the end of S phase and in cell-free mRNA decay reaction mixtures supplemented with histone proteins, indicating that histones might autoregulate the histone mRNA half-life. Histone mRNA destabilization in vitro requires three components: polysomes, histones, and postpolysomal supernatant (S130). Polysomes are the source of the mRNA and mRNA-degrading enzymes. To investigate the role of the S130 in autoregulation, crude S130 was fractionated by histone-agarose affinity chromatography. Two separate activities affecting the histone mRNA half-life were detected. The histone-agarose-bound fraction contained a histone mRNA destabilizer that was activated by histone proteins; the unbound fraction contained a histone mRNA stabilizer. Further chromatographic fractionation of unbound material revealed only a single protein stabilizer, which was purified to homogeneity, partially sequenced, and found to be La, a wellcharacterized RNA-binding protein. When purified La was added to reaction mixtures containing polysomes, a histone mRNA decay intermediate was stabilized. This intermediate corresponded to histone mRNA lacking 12 nucleotides from its 3 end and containing an intact coding region. Anti-La antibody blocked the stabilization effect. La had little or no effect on several other cell cycle-regulated mRNAs. We suggest that La prolongs the histone mRNA half-life during S phase and thereby increases histone protein production.

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Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.

Abstract: Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus (Epstein-Barr virus-encoded RNA/La antigen/small nuclear ribonucleoprotein)

Cited by 521 publications

References 26 publications

Decreased expression of the C3b/C4b receptor (CR1) and the C3d receptor (CR2) on B lymphocytes and of CR1 on neutrophils of patients with systemic lupus erythematosus

Decreased expression of the C3b/C4b receptor (CR1) and the C3d receptor (CR2) on B lymphocytes and of CR1 on neutrophils of patients with systemic lupus erythematosus

Eukaryotic regulatory RNAs: an answer to the ‘genome complexity’ conundrum

Human La Protein: a Stabilizer of Histone mRNA

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