Two species of the Asian endemic genus Keteleeria form ectomycorrhizas with diverse fungal symbionts in southwestern China

Abstract

The ectomycorrhizal status of Keteleeria species is reported for the first time based on morphological and molecular analyses of root tips from southwestern China. Based on internal transcribed spacer rDNA sequences, we detected 26 ectomycorrhizal (ECM) fungal species on roots of Keteleeria evelyniana and Keteleeria davidiana collected from natural sites and a botanical garden in Kunming, China. These ECM symbionts represent six fungal lineages, including /russula-lactarius, /inocybe, /sebacina, /tomentella-thelephora, /wilcoxina, and /cenococcum. Our results provide the first evidence of ECM formation by Keteleeria and also supply ecologically important information for conservation and restoration efforts to recover populations of Keteleeria.

Enigmatic Sebacinales

“…was recorded from Pinus sylvestris on serpentine soil in East Austria by Urban et al (2008). Among ectomycorrhizal fungi of Keteleeria davidiana and K. evelyniana of Southwest China, sebacinoids were also present (Ge et al 2011). Reithmeier (2011 studied the facilitation of ectomycorrhizal colonization of Picea mariana by alternate host plants above treeline in Labrador.…”

Six New Species of Tomentella (Thelephorales, Basidiomycota) From Tropical Pine Forests in Central Vietnam

“…The combined dataset of the 276 sequences had an aligned length of 1683 sites with 643-bp ITS and 1040-bp LSU, which included 955 constant characters, 109 parsimony-uninformative variable characters, and 619 parsimony informative positions. The multiple sequence alignment included the following: 12 sequences of the 6 new species; 10 sequences of 5 Thelephora species ( Kõljalg et al, 2000 ; Tedersoo et al, 2014 ; Larsson et al, 2019 ); 199 sequences of 101 other Tomentella species ( Larsson et al, 2004 ; Tedersoo et al, 2006 ; Smith et al, 2007 ; Yorou et al, 2007 , 2011 ; Morris et al, 2008 ; Yorou and Agerer, 2008 ; Suvi et al, 2010 ; Richard et al, 2011 ; Ge et al, 2012 ; Peintner and Dämmrich, 2012 ; Huang et al, 2014 ; Disyatat et al, 2016 ); 53 sequences of Tomentella spp. from China, Vietnam, and Thailand ( Wang et al, 2011 , 2012 ; Ge et al, 2012 ; Huang et al, 2014 ; Kaewgrajang et al, 2014 ; Tedersoo et al, 2014 ; Disyatat et al, 2016 ); and 2 outgroup sequences of Odontia ferruginea from Estonia ( Tedersoo et al, 2014 ; Yuan et al, 2018 ).…”

A molecular analysis reveals hidden species diversity within the current concept of Russula maculata (Russulaceae, Basidiomycota)

“…Two sequences from outside Europe (UDB013256 from a Papua New Guinea, from a fruitbody collection, recorded with Castanopsis acuminatissima, and KR082870 from China with no published ecological details) have the same monophyletic lineage, but sufficient phylogenetic support does not exist to conclude whether they are conspecific with the Pakistani species. The other Asian sequence (JN129407 from China) was generated from a mycorrhizal root tip of Keteleeria (Pinaceae) (Ge et al 2012) and possibly represents a distinct, undescribed species. Because of geographical distance, ecological difference, ITS variation and paraphyly (Fig.…”

“…Gminder and co-workers also reported occurrences in conifer plantations. The species is also known from Asia, but there, it is found in association with conifers as follows: with pines in Pakistan (Sultana et al 2011) and with Keteleeria evelyniana (Pinaceae) in China (Ge et al 2012). Das et al (2006) described an Indian species associated with Quercus, R. mayawatiana Das, Miller & Sharma (2006: 206), and they placed it close to R. maculata according to ITS data (the nrDNA internal transcribed spacer region) despite its lack of yellow-brownish spots.…”