Two species of Ulva inhibits the progression of cervical cancer cells SiHa by means of autophagic cell death induction

Pal, Asmita; Verma, Preeti; Paul, Subhabrata; Majumder, Indira; Kundu, Rita

doi:10.1007/s13205-020-02576-9

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…This finding was consistent with a previous report of autophagy inducing effect of HDHE in MDA-MD-231 human breast cancer cells at the same concentration [7]. A previous study found that palmitic acid-enriched chloroform extracts of green algae Ulva intestinalis and Ulva lactuca could induce autophagic cell death in SiHa cervical cancer cells [17].…”

Section: Promotion Of Cellular Autophagysupporting

confidence: 93%

Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies

Vivithanaporn,

Siangcham,

Tanawoot

et al. 2023

Trends Sci

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Glioblastoma (GBM) considered as aggressive brain cancer with high mortality rate in patients even after surgical resection. Resistant to chemotherapy is the major problem in GBM therapy. Discovery of novel bioactive compounds from algae is being investigated as alternative sources for potential treatment as well as prevention in glioblastoma. This study revealed the effects of marine red algae extract from hexane solvent fraction of Halymenia durvillei (HDHE) on proliferation and cell death in A172 human GBM cells. HDHE decreased proliferation and promoted cell cycle arrest at G2/M phase. HDHE induced apoptotic cell death in A172 cells through mitochondrial membrane dysfunction, the decrease of anti-apoptotic Bcl-2 protein expression, and activation of caspase 3/7. Moreover, HDHE increased intracellular reactive oxygen species (ROS) production and accumulation of LC3-II, an autophagic marker. The docked conformation of palmitic acid, a major component of HDHE, showed a high affinity binding to TP53 and Beclin-1 as cell death-related target molecules. This research conclusively demonstrated that HDHE might serve as a potent anticancer agent against glioblastoma by promoting apoptotic and autophagic cell death in A172 human GBM cells. HIGHLIGHTS The palmitic acid-enriched extract of red alga Halymenia durvillei (HDHE) could inhibit proliferation of A172 human glioblastoma cells by arresting cell cycle at G2/M phase HDHE could induce both caspase-dependent apoptotic and autophagic death of A172 cells Palmitic acid, a major component of HDHE, showed a high affinity binding to TP53 and Beclin-1 The extract could be beneficial to develop as chemopreventive agents or food supplements against glioblastoma GRAPHICAL ABSTRACT

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Section: Promotion Of Cellular Autophagysupporting

confidence: 93%

Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies

Vivithanaporn,

Siangcham,

Tanawoot

et al. 2023

Trends Sci

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“…Additionally, like us, Nazarudin et al [ 107 ] reported that the Chlorophyte U. intestinalis inhibited growth of liver HepG2 cancer cells. In addition, U. intestinalis extract inhibited cervical cancer cells by autophagy induction through increases of p53, Bax, atg12, and p62 proteins [ 108 ]. Furthermore, lipid extracts from U. rigida exhibited inhibition of breast MDA-MB-231 cancer cells [ 109 ].…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Activity and Inhibition of Liver Cancer Cells’ Growth of Extracts from 14 Marine Macroalgae Species of the Mediterranean Sea

Goutzourelas

Kevrekidis

Barda

et al. 2023

Foods

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Macroalgae exhibit beneficial bioactivities for human health. Thus, the aim of the present study was to examine the antioxidant and anticancer potential of 14 macroalgae species’ extracts, namely, Gigartina pistillata, Gigartina teedei, Gracilaria gracilis, Gracilaria sp., Gracilaria bursa pastoris, Colpomenia sinuosa, Cystoseira amentacea, Cystoseira barbata, Cystoseira compressa, Sargassum vulgare, Padina pavonica, Codium fragile, Ulva intestinalis, and Ulva rigida, from the Aegean Sea, Greece. The antioxidant activity was assessed using DPPH, ABTS•+, •OH, and O2•− radicals’ scavenging assays, reducing power (RP), and protection from ROO•-induced DNA plasmid damage assays. Moreover, macroalgae extracts’ total polyphenol contents (TPCs) were assessed. Extracts’ inhibition against liver HepG2 cancer cell growth was assessed using the XTT assay. The results showed that G. teedei extract’s IC50 was the lowest in DPPH (0.31 ± 0.006 mg/mL), ABTS•+ (0.02 ± 0.001 mg/mL), •OH (0.10 ± 0.007 mg/mL), O2•− (0.05 ± 0.003 mg/mL), and DNA plasmid breakage (0.038 ± 0.002 mg/mL) and exhibited the highest RP (RP0.5AU 0.24 ± 0.019 mg/mL) and TPC (12.53 ± 0.88 mg GAE/g dw). There was also a significant correlation between antioxidant activity and TPC. P. pavonica (IC50 0.93 ± 0.006 mg/mL) exhibited the highest inhibition against HepG2 cell growth. Conclusively, some of the tested extracts exhibited significant chemopreventive properties, and so they may be used for food products.

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“…In recent years, the regulation of autophagy has received much attention in relation to cancer development and treatment ( Yamazaki et al, 2021 ), Ulva intestinalis and Ulva lactuca have been shown to induce autophagic cell death in SiHa cells by up-regulating the expression of Bax and p53 and downregulating the expression of Bcl2, and they also reduced the expression of the viral oncogene HPV-E6 by 1.8 to 2-fold. Both algae were characterized and analyzed by GC-MS, and the main active component of both was palmitic acid ( Pal et al, 2021 ; Wang et al, 2023a ). However, Gu et al found that berberine can cause substantial antiproliferative effects on the cervical cancer cell line HeLa by inhibiting intracellular fatty acid synthesis through the activation of AMPK, thereby decreasing the activity of acetyl coenzyme a carboxylase, and exogenous supplementation with palmitic acid rescued this inhibitory effect ( Gu et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Plasma free fatty acid levels in cervical cancer: concurrent chemoradiotherapy improves abnormal profile

Xu,

Ping,

Zhang

et al. 2024

Front. Pharmacol.

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Background: Epidemiology has demonstrated that plasma free fatty acids (FFAs) can prevent the development of cancer. Our study sought to evaluate the relationship between plasma (FFA) levels and cervical cancer.Methods: In recent years, metabolomics-based approaches have been recognized as an emerging tool, so we examined the plasma FFA profiles of 114 patients with cervical cancer and 151 healthy people using liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods.Results: The data results were analyzed by multifactorial binary logistic regression analysis, and it was found that palmitic acid, docosahexaenoic acid (DHA), and total ω-3 fatty acids were negatively correlated with the risk of cervical cancer; whereas tetracosanoic acid was positively correlated with the risk of cervical cancer (OR, 1.026; 95% CI, 1.013–1.040; p < 0.001). Dynamic follow-up of 40 cervical cancer patients who successfully completed CCRT revealed that most fatty acid levels tended to increase after the end of treatment, except for palmitic and stearic acid levels, which were lower than before treatment.Conclusion: Plasma FFA profiles were altered in cervical cancer patients, which may be related to abnormal fatty acid metabolism in cervical cancer. The described changes in fatty acid profiles during CCRT may be related to the good functioning of CCRT. Further studies on plasma FFA composition and its changes due to CCRT in patients with cervical cancer are warranted.

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Two species of Ulva inhibits the progression of cervical cancer cells SiHa by means of autophagic cell death induction

Cited by 6 publications

References 38 publications

Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies

Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies

Antioxidant Activity and Inhibition of Liver Cancer Cells’ Growth of Extracts from 14 Marine Macroalgae Species of the Mediterranean Sea

Plasma free fatty acid levels in cervical cancer: concurrent chemoradiotherapy improves abnormal profile

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