Two-stage CSTR–UASB digestion enables superior and alkali addition-free cheese whey treatment

Diamantis, Vasileios; Kapagiannidis, Anastasios G.; Ntougias, Spyridon; Tataki, Vasiliki; Melidis, Paraschos; Aivasidis, A.

doi:10.1016/j.bej.2014.01.001

Cited by 47 publications

(32 citation statements)

References 34 publications

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“…In the only such study found to date, Kim et al (2008) reported lactate and ethanol as the main metabolic products of cheese whey fermentation. Despite the high production of propionate (Table 1), the biogas production rate in the UASB reactor was equal to 4.31 ± 0.53 L L −1 day −1 as the result of the enhanced acidification strategy followed and the two-stage configuration used (Diamantis et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The system consisted of an acidogenic CSTR accompanied with a clarifier for biomass settlement and a methanogenic UASB reactor (Diamantis et al 2014). The CSTR was initially filled with biomass which had spontaneously developed in a tank used for long-term cheese whey storage, whereas the UASB reactor was fed with granular sludge obtained from the cheese whey factory.…”

Section: Methodsmentioning

confidence: 99%

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Dominance of rumen microorganisms during cheese whey acidification: acidogenesis can be governed by a rare Selenomonas lacticifex-type fermentation

Ntougias

Tsiamis

Soultani

et al. 2015

Appl Microbiol Biotechnol

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The microbial basis of acidification process during spontaneous cheese whey wastewater fermentation was decrypted by implementing both culture-dependent and culture-independent techniques. Lac tobacillus and Bifidobacterium were the predominant taxa among the microbiota growing on MRS (deMan, Rogosa, and Sharpe), while Kazachstania unispora and Dekkera anomala yeast species were also isolated. Almost all Lactobacillus isolates were heterofermentative that could ferment glucose and lactose, with most of them being related to Lactobacillus hilgardii (99.0-100 % similarity). By employing fluorescence techniques, the dominance of long crescent-shaped bacteria in the acidogenic sludge was observed. Temperature gradient gel electrophoresis (TGGE), clone library, and next-generation sequencing techniques revealed the dominance of Selenomonas lacticifex. Based on Illumina data, Selenomonas in the continuous stirred-tank reactor (CSTR) represented 70.13 ± 4.64 % of the bacterial reads, while other Veillonellaceae taxa (Megasphaera and Pectinatus) represented a notable proportion (6.54 %). Prevotella was only detected by Illumina sequencing as an important constituent of the microbial population (14.97 ± 1.71 %). Budding yeasts represented 97 % of the fungal population in the CSTR, with Yarrowia strains representing 88.85 ± 5.52 % of the fungal reads. Spontaneous cheese whey acidification can favor the dominance of rumen bacteria and here was driven by the rarely reported S. lacticifex-type fermentation, which should be taken into consideration during evaluation of acidogenesis in process simulation and modelling. Moreover, the important nervonic acid content detected indicates that acidogenic sludge can be used as a source for the production of high value-added biomedical substrates.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Dominance of rumen microorganisms during cheese whey acidification: acidogenesis can be governed by a rare Selenomonas lacticifex-type fermentation

Ntougias

Tsiamis

Soultani

et al. 2015

Appl Microbiol Biotechnol

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“…Empleando un sistema compuesto por dos reactores tipo CSTR, que incorpora una unidad de filtración de membrana para la recirculación en la segunda etapa, se consiguió operar con cargas de hasta 19,8gDQO/Ld con un eliminación de DQO del 79% [84]. Diamantis et al [85], evaluaron un sistema de dos fases compuesto de un reactor CSTR para la acidificación, seguido de un reactor UASB para la metanización. Estos autores alcanzaron conversiones de biomasa de hasta un 90% con rendimientos de producción de metano de 0,37m 3 CH 4 /kgDQOeliminado.…”

Section: Producción De Hidrógenounclassified

Procesos biológicos para el tratamiento de lactosuero con producción de biogás e hidrógeno. Revisión bibliográfica

Rodríguez

Torres

Palao

et al. 2016

rev.ion

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ResumenLa industria láctea se caracteriza por generar lactosuero como subproducto del proceso de elaboración del queso. El lactosuero presenta un elevado contenido en materia orgánica en forma de lactosa, proteínas y otros compuestos procedentes de la leche. Su vertido incontrolado puede ocasionar problemas de contaminación con un grave impacto ambiental. Sin embargo, la recuperación de algunos componentes del lactosuero permite valorizar este subproducto, encontrando aplicación en la industria alimentaria, farmacéutica y recientemente en la conversión del lactosuero para la producción de biocombustibles. Actualmente, los biocombustibles son una fuente potencial de energía renovable con un papel importante como posibles sustitutos de los combustibles fósiles. Por este motivo, la opción de gestionar el lactosuero mediante una valorización energética ha adquirido gran interés durante los últimos años. Entre las alternativas disponibles de aprovechamiento energético, se encuentra la digestión anaerobia, la cual es una de las tecnologías más empleadas para el tratamiento de diverso tipo de residuos. En el caso del lactosuero, se han desarrollado múltiples trabajos en los que se han utilizado diferentes confi guraciones y condiciones de operación con el objetivo de mejorar el funcionamiento del proceso. Gracias a estos trabajos se han podido identifi car algunos de los factores que limitan su aplicación como son: la tendencia a la acidifi cación o la limitación de nutrientes. Por este motivo se han promovido otras alternativas como son los procesos de co-digestión con varios materiales o la fermentación oscura. El objetivo de este artículo es la realización de una revisión bibliográfi ca de los principales trabajos realizados en los que se contemplan los procesos biológicos anteriormente mencionados. Se presentan los resultados más relevantes, así como la infl uencia de las condiciones de operación y los factores que afectan a la producción de biogás. AbstractDairy industry is characterized by high generation of cheese whey, a byproduct from cheese factories with a high organic matter content in the form of lactose, proteins and other compounds coming from processed milk. If it is not properly disposed it might favour serious environmental pollution problems. However, it is possible the application of recovery technologies in order to obtain its main compounds for further applications such as food or pharmaceutical industry, and for obtaining biofuels. Nowadays, biofuels are an important source of renewable energy as potential future alternative for fossil fuels. For this reason, 47Cita: Fernández Rodríguez C, Martínez Torres EJ, Morán Palao A, Gómez Barrios X. Procesos biológicos para el tratamiento de lactosuero con producción de biogás e hidrógeno. Revisión bibliográfi ca. rev.ion. 2016;29(1):47-62.

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“…Diamantis et al [24] developed a two-stage CSTR-UASB system without any requirements for alkali consumption for the anaerobic treatment of diluted cheese whey with dairy-washing waters.…”

Section: Introductionmentioning

confidence: 99%

“…In this regard Spachos and Stamatis [14] performed an exergetic and economic analysis of an anaerobic treatment system of whey accompanied by the production of steam, concluding that the anaerobic treatment of whey can be a sustainable investment, at least for a substantial volume of whey. The anaerobic treatment of cheese whey has been well reported by several authors under different systems and conditions, mainly in high-rate anaerobic systems, such as the upflow anaerobic sludge blanket (UASB) reactor [11,[15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

High-load anaerobic co-digestion of cheese whey and liquid fraction of dairy manure in a one-stage UASB process: Limits in co-substrates ratio and organic loading rate

Rico

Muñoz

Fernández

et al. 2015

Chemical Engineering Journal

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Please cite this article as: C. Rico, N. Muñoz, J. Fernández, J.L. Rico, High-load anaerobic co-digestion of cheese whey and liquid fraction of dairy manure in a one-stage UASB process: limits in co-substrates ratio and organic loading rate, Chemical Engineering Journal (2014), doi: http://dx. AbstractCheese whey is considered an important pollutant in dairy wastewaters and an environmental problem to solve. This study aimed to develop a treatment process that combines the advantages of co-digesting cheese whey with manure and the short hydraulic retention time of a high-load anaerobic treatment system by using liquid fractions of dairy manure as a co-substrate. The proposed co-digestion process consists of a one-stage UASB reactor with an external settler and effluent recycling for alkalinity supplementation. Under a constant hydraulic retention time of 2.2 days and increasing proportion of cheese whey in the feed, the system demonstrated stable operation up to a 75% cheese whey fraction in the feed, with an applied organic loading rate of 19.4 kg COD m -3 d -1 , obtaining a 94.7% COD removal and a volumetric methane production rate of 6.4 m 3 CH 4 m -3 d -1 . Critical biomass washout was experienced when the cheese whey fraction in the feed was 85%.Operation at a constant 60% cheese whey fraction in the feed mixture enabled stable operation under an organic loading rate of 28.7 kg COD m -3 d -1 and 1.3 days HRT, with 95.1% COD removal and a volumetric methane production rate of 9.5 m 3 CH 4 m -3 d -1 . This new high-load co-digestion method proposed is a promising solution for areas where cheese factories and intensive livestock farming are responsible for environmental pollution caused by unsuitable cheese whey and manure management practices.

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Two-stage CSTR–UASB digestion enables superior and alkali addition-free cheese whey treatment

Cited by 47 publications

References 34 publications

Dominance of rumen microorganisms during cheese whey acidification: acidogenesis can be governed by a rare Selenomonas lacticifex-type fermentation

Dominance of rumen microorganisms during cheese whey acidification: acidogenesis can be governed by a rare Selenomonas lacticifex-type fermentation

Procesos biológicos para el tratamiento de lactosuero con producción de biogás e hidrógeno. Revisión bibliográfica

High-load anaerobic co-digestion of cheese whey and liquid fraction of dairy manure in a one-stage UASB process: Limits in co-substrates ratio and organic loading rate

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