Two-stage microbial conversion of crude glycerol to 1,3-propanediol and polyhydroxyalkanoates after pretreatment

Pan, Chaozhi; Tan, Giin-Yu Amy; Ge, Liya; Chen, Chia Lung; Wang, Jing Yuan

doi:10.1016/j.jenvman.2018.11.118

Cited by 26 publications

(5 citation statements)

References 40 publications

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“…Untuk produksi biodiesel setiap ton di peroleh 100 kg crude gliserol. Crude gliserol merupakan cairan yang sangat kental dengan warna coklat gelap dan memiliki pH 9,6 [18]. Crude gliserol mengandung impuritis seperti garam anorganik, MONG dan air.…”

Section: Teoriunclassified

Pemanfaatan Limbah Cangkang Telur Ayam Sebagai Adsorben Pada Pemurnian Gliserol Dengan Metode Asidifikasi Dan Adsorpsi

Nadeak

Hasibuan²,

Naibaho³

et al. 2019

J. Teknik Kimia

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AbstrakGliserol sebagai hasil samping produksi biodiesel rata-rata terbentuk 10-20% dari berat biodiesel. Pengotor yang terkandung dalam crude gliserol seperti garam anorganik, katalis, air serta matter organic non glycerol (MONG) yang mengandung asam lemak bebas, asam lemak sisa metil ester, gliserida, dan alkohol (umumnya metanol atau etanol) memberikan pengaruh signifikan pada konsentrasi gliserol. Untuk itu diperlukan beberapa perlakuan terutama untuk menghilangkan impuritisnya. Penelitian ini bertujuan untuk memurnikan gliserol dengan metode asidfikasi menggunakan fosfat dan adsorpsi dengan limbah cangkang telur ayam. Percobaan diawali dengan pretreatment gliserol dengan asam fosfat dengan variabel uji rasio b e r a t (w/w) asam yang ditambahkan (1:0,2; 1:0,4; 1:0,6; 1:0,8 dan 1:1), dilanjutkan dengan adsorpsi menggunakan limbah cangkang telur ayam yang sudah diaktivasi secara termal dengan variabel uji persen berat adsorben (%w/w) 3%, 6%, 9%, 12% dan 15% dengan kondisi reaksi kecepatan pengadukan 250 rpm dan waktu adsorpsi 120 menit. Hasil kemurnian terbaik didapatkan pada rasio berat (w/w) asidifikasi 1:0,6, persen berat adsorben (%w/w) 15% dengan kadar gliserol sebesar 67,22%, densitas 1,171 g/cm 3 , kadar air 2,796%, kadar abu 13,852% dan kadar MONG 16,130%.Kata kunci: crude gliserol, MONG, asidifkasi, adsorpsi. AbstractGlycerol as a byproduct of biodiesel production was approximately formed 10-20% of the biodiesel weight. Impurities which contained in the glycerol such as inorganic salts, catalysts, water and matter organic non glycerol (MONG) which contained free fatty acids, fatty acids as residual of methyl ester, glycerides, and alcohols (generally methanol or ethanol) had a significant effect on glycerol concentration. We needed some treatment, especially to eliminate the impuritis. This study aims to purify glycerol by acidfication using phosphate and adsorption with chicken egg shell waste. This research was begun with the pretreatment of glycerol using phosphoric acid with the variable of test weight ratio (w/w) acid added 1: 0.2; 1: 0.4; 1: 0.6; 1: 0.8 and 1: 1 , continued with adsorption using thermally activated chicken egg shell waste with the variable weight percent adsorbent (%w/w) 3%, 6%, 9%, 12% and 15% with reaction conditions, stirring speed 250 rpm and adsorption time 120 minutes. The purest result was obtained at weight ratio (w/w) acidification of 1: 0.6, weight percent adsorbent (% w/w) 15% with glycerol content of 67.22%, density 1.171 g / cm3, moisture content 2.796%, ash content 13.852% and MONG content 16.130%.

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Section: Teoriunclassified

Pemanfaatan Limbah Cangkang Telur Ayam Sebagai Adsorben Pada Pemurnian Gliserol Dengan Metode Asidifikasi Dan Adsorpsi

Nadeak

Hasibuan²,

Naibaho³

et al. 2019

J. Teknik Kimia

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“…In general, the effect of impurities on bioprocess success depends on the origin of the crude glycerol and its concentration in the cultivation medium (Hejna et al 2016). Possible solutions to overcome these problems are the use of microorganisms that are tolerant to the impurities in crude glycerol (Konstantinović et al 2016), the application of appropriate treatments (Pan et al 2019) and the implementation of fed-batch cultivation systems in which crude glycerol is added gradually (Samul et al 2014). However, all of these activities can decrease the economic potential of processes that utilise crude glycerol as the raw material (Rahim et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Optimisation of xanthan production on glycerol-based medium using response surface methodology

Rončević

Bajić

Vlajkov

et al. 2020

Braz. J. Chem. Eng.

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Xanthan is one of the most important biopolymers characterised by a high commercial value. Since the availability of essential nutrients influences its biosynthesis, the aim of this study was the optimisation of carbon, organic and inorganic nitrogen content in glycerol-based medium employing response surface methodology. The cultivation of strain Xanthomonas campestris ATCC 13951 was carried out under appropriate conditions on media with glycerol, peptone and ammonium-nitrate formulated according to Box-Behnken design (3 3), while the desirability function approach was used for determination of optimal nutrient levels. The final model predicts that the maximal amount of xanthan (12.95 g/L) is produced when the initial contents of glycerol, peptone and ammonium-nitrate in the medium are 32.96 g/L, 0.55 g/L and 0.73 g/L, respectively. To minimize the residual nutrient content and therefore the costs of effluent processing, additional optimisation was performed. In order to validate the optimisation model developed and examine the bioprocess success with crude glycerol as the sole carbon source, additional experiments were performed. The results represent reliable information for further investigations.

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“…Other authors investigated this phenomenon and determined that the fatty acid fraction of crude glycerol enters a stable colloidal state between pH 11.1 and 6.4 due to the decrease below the pKa values for C 16 and C 18 fatty acids. pH values below 6.4 determined the aggregation of the colloid into a floating layer [20]. Both studies in which this phenomenon was observed chose to further purify the CG, this fraction having had an inhibiting effect on the bacterial culture [19].…”

Section: Comparison Of the Pha Accumulation Of Strain C22 Using Pure ...mentioning

confidence: 99%

Revealing the Phenotypic and Genomic Background for PHA Production from Rapeseed-Biodiesel Crude Glycerol Using Photobacterium ganghwense C2.2

Lascu

Tanase

Jablonski

et al. 2022

IJMS

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Polyhydroxyalkanoates (PHA) are promising biodegradable and biocompatible bioplastics, and extensive knowledge of the employed bacterial strain’s metabolic capabilities is necessary in choosing economically feasible production conditions. This study aimed to create an in-depth view of the utilization of Photobacterium ganghwense C2.2 for PHA production by linking a wide array of characterization methods: metabolic pathway annotation from the strain’s complete genome, high-throughput phenotypic tests, and biomass analyses through plate-based assays and flask and bioreactor cultivations. We confirmed, in PHA production conditions, urea catabolization, fatty acid degradation and synthesis, and high pH variation and osmotic stress tolerance. With urea as a nitrogen source, pure and rapeseed-biodiesel crude glycerol were analyzed comparatively as carbon sources for fermentation at 20 °C. Flask cultivations yielded 2.2 g/L and 2 g/L PHA at 120 h, respectively, with molecular weights of 428,629 g/mol and 81,515 g/mol. Bioreactor batch cultivation doubled biomass accumulation (10 g/L and 13.2 g/L) in 48 h, with a PHA productivity of 0.133 g/(L·h) and 0.05 g/(L·h). Thus, phenotypic and genomic analyses determined the successful use of Photobacterium ganghwense C2.2 for PHA production using urea and crude glycerol and 20 g/L NaCl, without pH adjustment, providing the basis for a viable fermentation process.

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Two-stage microbial conversion of crude glycerol to 1,3-propanediol and polyhydroxyalkanoates after pretreatment

Cited by 26 publications

References 40 publications

Pemanfaatan Limbah Cangkang Telur Ayam Sebagai Adsorben Pada Pemurnian Gliserol Dengan Metode Asidifikasi Dan Adsorpsi

Pemanfaatan Limbah Cangkang Telur Ayam Sebagai Adsorben Pada Pemurnian Gliserol Dengan Metode Asidifikasi Dan Adsorpsi

Optimisation of xanthan production on glycerol-based medium using response surface methodology

Revealing the Phenotypic and Genomic Background for PHA Production from Rapeseed-Biodiesel Crude Glycerol Using Photobacterium ganghwense C2.2

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