Two-step Enzymatic Synthesis of Guanine Arabinoside

The aim of this work was to explore the possibility of the application of cloud point extraction (CPE) method in micelle media to recovery betaine from beet molasses. Response surface method was employed to assess the effects of surfactant concentration, molasses concentration, incubation time, pH, electrolyte concentration, mixing time, and surfactant type on efficiency of betaine recovery from beet molasses. Also, a mathematical model was developed to predict the effect of each variable and their interactions on the efficiency of betaine recovery. The model showed that best surfactant was Triton X-114 and under the optimum conditions, betaine recovery from beet molasses was achieved up to 80% when three CPE steps with total of 1.5% (w/v) of surfactant were used. Subsequently, betaine was recovered nearly 100% from surfactant rich phase after adjusting pH at 2.5 and re-incubation at 40 °C. The results showed that the proposed method is suitable for extraction of betaine from beet molasses.

show abstract

Use of Citrobacter koseri whole cells for the production of arabinonucleosides: A larger scale approach

Nóbile

Médici

Terreni

et al. 2012

Process Biochemistry

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Two-step Enzymatic Synthesis of Guanine Arabinoside

Cited by 3 publications

References 13 publications

Citrobacter koseri immobilized on agarose beads for nucleoside synthesis: a potential biocatalyst for preparative applications

Citrobacter koseri immobilized on agarose beads for nucleoside synthesis: a potential biocatalyst for preparative applications

A new approach for separation and recovery of betaine from beet molasses based on cloud point extraction technique

Use of Citrobacter koseri whole cells for the production of arabinonucleosides: A larger scale approach

Contact Info

Product

Resources

About