Two-step partial nitritation/Anammox process in granulation reactors: Start-up operation and microbial characterization

Dosta, J.; Vilà, Joaquim; Sancho, I.; Basset, N.; Grifoll, Magdalena; Mata‐Alvarez, J.

doi:10.1016/j.jenvman.2015.08.023

Cited by 55 publications

(22 citation statements)

References 46 publications

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“…B. sinica has greater sensitivity to SHARON effluent. Similar relative abundance of anammox bacteria was also detected previously when ANAMMOX reactors were fed with pre-treated wastewater from partial nitrification [7.9%, Dosta et al ( 2015 ); 0.3%, Wang et al ( 2016 )]. The decline of relative abundance of anammox bacteria might be due to the ambiguous compositions in piggery wastewater, e.g., antibiotics, heavy metals, phosphate and sulfide (Bernet et al 2000 ; Tang et al 2011 ; Tong et al 2009 ; Jin et al 2012 ).…”

Section: Discussionsupporting

confidence: 80%

Microbial community dynamics in an ANAMMOX reactor for piggery wastewater treatment with startup, raising nitrogen load, and stable performance

Huang

Miao

et al. 2018

AMB Expr

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Bacterial community dynamics of the ANAMMOX reactor of an integrated “UASB + SHARON + ANAMMOX” system for treating piggery wastewater were investigated using the Illumina MiSeq method with samples obtained at ~ 2-week intervals during a 314-day period. With aerobic activated sludge as seeds and low content artificial wastewater (NH4+–N 50 mg/L; NO2−–N 55 mg/L) as influent for the ANAMMOX reactor, nitrogen removal was initially observed on day 38 with a removal rate 1.3 mg N L−1 day−1, and increased to 90.4 mg N L−1 day−1 on day 55 with almost complete removal of ammonia and nitrite, indicating a successful startup of the reactor. Increasing influent load stepwise to NH4+–N 272.7 mg/L/NO2−–N 300 mg/L, nitrogen removal rate increased gradually to 470 mg N L−1 day−1 on day 228, and maintained a stable level (~ 420 mg N L−1 day−1) following introduction of SHARON effluent since day 229. Correlation between microbial community dynamics and nitrogen removal capability was significant (r = 0.489, p < 0.001). Microbial community composition was determined by influent ammonia, influent nitrite, effluent nitrate and some undefined factors. Anammox bacteria, accounting for ~ 98.7% of Planctomycetes, became detectable (0.03% relative abundance) since day 38 and increased to 0.9% on day 58, well consistent with nitrogen removal performance of the reactor. Relative abundance of anammox bacteria gradually increased to 38.4% on day 140 with stepwise increased influent load; decreased to 0.4% on day 169 because of nitrite inhibition; increased to 19.24% on day 233 when the influent load was dropped; kept at ~ 9.0% with SHARON effluent used as influent and dropped to 3.3% finally. Anammox bacteria, only Candidatus Brocadia and Ca. Kuenenia detected, were the most abundant at genus level. Ca. Brocadia related taxa were enriched firstly under low load and detectable during the entire experimental period. Three main groups represented by Ca. Brocadia related OTUs were enriched or eliminated at different loads, but Ca. Kuenenia related taxa were enriched only under high load (NO2−–N > 300 mg/L), suggesting their different niches and application for different loads. These findings improve the understanding of relationships among microbial community/functional taxa, running parameters and reactor performance, and will be useful in optimizing running parameters for rapid startup and high, stable efficiency.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0686-0) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 80%

Microbial community dynamics in an ANAMMOX reactor for piggery wastewater treatment with startup, raising nitrogen load, and stable performance

Huang

Miao

et al. 2018

AMB Expr

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“…In previous studies, denitrification and even chemolithotrophic denitrification by Proteobacteria, Chlorobi and Chloroflexi have been detected 25 26 27 28 . Proteobacteria, Chloroflexi, Bacteroidetes and Chlorobi were also the main dominant phyla in previous nitritation studies 29 30 . With the exception of S2 (52.11%), the relative abundance of Bacteroidetes decreased significantly from 38.57% (S1) to 4.41% (S8), which was less abundant than those reported studies regarding to nitritation process 29 30 .…”

Section: Resultsmentioning

confidence: 55%

“…Proteobacteria, Chloroflexi, Bacteroidetes and Chlorobi were also the main dominant phyla in previous nitritation studies 29 30 . With the exception of S2 (52.11%), the relative abundance of Bacteroidetes decreased significantly from 38.57% (S1) to 4.41% (S8), which was less abundant than those reported studies regarding to nitritation process 29 30 . Interestingly, the relative abundance of the NOB Nitrospirae increased from 2% (S1) to 3% (S2) after 7 days.…”

Section: Resultsmentioning

confidence: 55%

Performance and microbial ecology of a nitritation sequencing batch reactor treating high-strength ammonia wastewater

Chen

Dai

Cao

et al. 2016

Sci Rep

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The partial nitrification (PN) performance and the microbial community variations were evaluated in a sequencing batch reactor (SBR) for 172 days, with the stepwise elevation of ammonium concentration. Free ammonia (FA) and low dissolved oxygen inhibition of nitrite-oxidized bacteria (NOB) were used to achieve nitritation in the SBR. During the 172 days operation, the nitrogen loading rate of the SBR was finally raised to 3.6 kg N/m3/d corresponding the influent ammonium of 1500 mg/L, with the ammonium removal efficiency and nitrite accumulation rate were 94.12% and 83.54%, respectively, indicating that the syntrophic inhibition of FA and low dissolved oxygen contributed substantially to the stable nitrite accumulation. The results of the 16S rRNA high-throughput sequencing revealed that Nitrospira, the only nitrite-oxidizing bacteria in the system, were successively inhibited and eliminated, and the SBR reactor was dominated finally by Nitrosomonas, the ammonium-oxidizing bacteria, which had a relative abundance of 83%, indicating that the Nitrosomonas played the primary roles on the establishment and maintaining of nitritation. Followed by Nitrosomonas, Anaerolineae (7.02%) and Saprospira (1.86%) were the other mainly genera in the biomass.

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“…As discussões apresentadas nos itens anteriores permitem a reflexão a respeito da combinação dos processos de nitritação parcial e Anammox, em termos de uma nova proposta de tecnologia para remoção de nitrogênio. Dá-se o nome de desamonificação a toda tecnologia que opere simultaneamente com ambos os processos: nitrificação parcial Capítulo VI -Tratamento do digestato -remoção de nitrogênio 175 e Anammox (De Prá et al, 2012;Dosta et al, 2015;Gilbert et al, 2015;Magrí et al, 2012).…”

Section: Processos Combinados De Desamonificaçãounclassified

Fundamentos da digestão anaeróbia, purificação do biogás, uso e tratamento do digestato

Kunz¹,

Steinmetz²,

Amaral³

2019

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Two-step partial nitritation/Anammox process in granulation reactors: Start-up operation and microbial characterization

Cited by 55 publications

References 46 publications

Microbial community dynamics in an ANAMMOX reactor for piggery wastewater treatment with startup, raising nitrogen load, and stable performance

Microbial community dynamics in an ANAMMOX reactor for piggery wastewater treatment with startup, raising nitrogen load, and stable performance

Performance and microbial ecology of a nitritation sequencing batch reactor treating high-strength ammonia wastewater

Fundamentos da digestão anaeróbia, purificação do biogás, uso e tratamento do digestato

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