Two strains ofVibrio cholerae non-O1 possessing somatic (O) antigen factors in common withV. cholerae serogroup O139 synonym “Bengal”

Shimada, Toshio; Arakawa, Eiji; Itoh, Kiyohiko; Nakazato, Tamotsu; Okitsu, Tadayuki; Yamai, Shiro; Kusum, Mayura; Nair, G. Balakrish; Takeda, Yoshifumi

doi:10.1007/bf01570225

Cited by 41 publications

(37 citation statements)

References 15 publications

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“…The Shimada procedure [2] was used for preparation of O-AgH. Brie¯y, the broth culture was heated at 1008C for 2 h and centrifuged at 2000 g for 15 min.…”

Section: O-ag Preparationsmentioning

confidence: 99%

“…Reference strain 169-68, representing serogroup O22, is used for absorption of O139-speci®c antiserum [2,4], because the O-Ag of this strain is closely related to that of strain O139 [2]. However, they are not completely identical [2], as the homology of rfb genes of V. cholerae O22 and O139 is 91% [16].…”

Section: Sds-page and Immunoblottingmentioning

confidence: 99%

“…Being simple, rapid and inexpensive, serotyping is used successfully for differentiation of vibrios within the Vibrionaceae and for epidemiological typing in outbreaks caused by V. cholerae O1 or O139, the second known aetiological agent of cholera [1]. Recently, diagnostic antiserum against V. cholerae O139 strain was obtained by immunisation of rabbits with heat-stable O139 antigen [2]. However, when tested for speci®city in the agglutination test it reacted with V. cholerae O22 reference strain 169-68 [2,4] and so for practical use needed special absorption.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, diagnostic antiserum against V. cholerae O139 strain was obtained by immunisation of rabbits with heat-stable O139 antigen [2]. However, when tested for speci®city in the agglutination test it reacted with V. cholerae O22 reference strain 169-68 [2,4] and so for practical use needed special absorption.…”

Section: Introductionmentioning

confidence: 99%

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Immunochemical characterisation of Vibrio cholerae O139 O antigens and production of a diagnostic antiserum without absorption

Feodorova¹,

Gromova

Devdariani³

et al. 2001

Journal of Medical Microbiology

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Rabbits and mice immunised with chemically extracted O-antigens (O-Ags) of Vibrio cholerae O139 (O-AgB and O-AgD) developed antibodies (Abs) which appeared to be highly speci®c in ELISA for the relevant antigens and V. cholerae O139 strains without absorption, in contrast to the Abs against the heated O-Ag (O-AgH). An ELISA test based on the use of these Abs was shown to detect V. cholerae O139 strains down to concentrations of (9.4 3 10 4 )±(7.5 3 10 5 ) vibrios=ml and demonstrated no cross-reaction with other vibrios including representatives of serogroup O22. Native and proteinase Ktreated O-AgB, O-AgD, O-AgH, as well as whole-cell lysates of V. cholerae O139 strains of different origin were used in immunoblotting with these Abs. Clear differences in the patterns of zones of speci®c reaction between chemically extracted and heated O-Ags and between lipopolysaccharide pro®les of the V. cholerae O139 strains of different origin were observed. Serogroup-speci®c protein bands in the native O-AgB and O-AgD preparations were de®ned. The approach described for obtaining serogroup-speci®c Abs against vibrios and other bacteria seems to be promising for the development of speci®c diagnostic tests and further investigation of bacterial antigenic structure.

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“…The Shimada procedure [2] was used for preparation of O-AgH. Brie¯y, the broth culture was heated at 1008C for 2 h and centrifuged at 2000 g for 15 min.…”

Section: O-ag Preparationsmentioning

confidence: 99%

Section: Sds-page and Immunoblottingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Immunochemical characterisation of Vibrio cholerae O139 O antigens and production of a diagnostic antiserum without absorption

Feodorova¹,

Gromova

Devdariani³

et al. 2001

Journal of Medical Microbiology

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“…Alditol acetates and V. cholerae O139 cross-reacts with a number of bacteria in-partially methylated alditol acetates were separated on an HP-5 cluding V. cholerae serogroups O22 and O155 [7] and Aeromo-fused-silica column (0.25 mmϫ30 m) with a temperature pronas trota [8]. Until now, only the A. trota O-antigen structure gram of 180°C for 1 min followed by 3°C change/min to 210°C.…”

mentioning

confidence: 99%

Structural analysis of the O‐antigenic polysaccharide from Vibrio mimicus N‐1990

Landersjö

Weintraub

Ansaruzzaman

et al. 1998

European Journal of Biochemistry

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The O-antigenic polysaccharide part of the lipopolysaccharide from Vibrio mimicus N-1990 has been investigated. Sugar and methylation analysis of native and dephosphorylated polysaccharide together with NMR spectroscopy show that the polysaccharide is composed of tetrasaccharide repeating units. The structure of the repeating unit of the polysaccharide from V. mimicus N-1990 could be determined as :The V. mimicus N-1990 strain cross-reacts with antibodies elicited against the Vibrio cholerae O139. The nature of this cross-reactivity resides in the partial structure comprising the galactosyl residue substituted with a cyclic phosphate. This element is present in the cell-wall-associated polysaccharides of both strains.Keywords : Vibrio mimicus ; polysaccharide ; structure ; cyclic phosphate ; NMR.Vibrio cholerae O139 Bengal emerged as a second aetiologic MATERIALS AND METHODS agent of epidemic cholera in the Indian subcontinent in 1992General methods. Anion-exchange chromatography was [1]. It has a lipopolysaccharide (LPS) with a short side chain [2] performed on a DEAE-Sepharose CL column (3 cmϫ20 cm, and produces a polysaccharide capsule [3]. Serological and ge-Pharmacia) eluted with a gradient from 0.1 M to 1 M NaCl. Gelnetic studies suggested that the capsular polysaccharide has the permeation chromatography (GPC) was performed on a Bio-Gel same repeating unit as the O-antigen chain [4, 5] and, thus, car-P-2 column irrigated with 0.4% acetic acid and 1 % pyridine ries the determinants of O-specificity. The capsular polysaccha-in water. Column eluents were monitored using a differential ride (CPS) has two unique constituents, a 3,6-dideoxy-L-xylo-refractometer (Waters) or by detection of total carbohydrates hexose (colitose) and a cyclic phosphate diester [6].using the phenol/sulfuric acid assay [10]. Alditol acetates and V. cholerae O139 cross-reacts with a number of bacteria in-partially methylated alditol acetates were separated on an HP-5 cluding V. cholerae serogroups O22 and O155 [7] and Aeromo-fused-silica column (0.25 mmϫ30 m) with a temperature pronas trota [8]. Until now, only the A. trota O-antigen structure gram of 180°C for 1 min followed by 3°C change/min to 210°C. has been determined [9]. The epitope that is responsible for the Hydrogen was used as carrier gas. The column was fitted to a above mentioned cross-reactivity involves the colitose residues Hewlett-Packard model 5890 gas chromatograph (Hewlett-Packpresent in the antigens of both strains. While searching for bac-ard) equipped with a flame ionisation detector. Gas-chromatogteria that cross-react with V. cholerae O139, we detected two raphy/mass-spectrometry (GC/MS) was performed using a Hewstrains of Vibrio mimicus that agglutinated with specific antise-lett-Packard model 5970 mass spectrometer. The GC/MS separarum to V. cholerae O139 in slide and tube agglutination tests tions were performed on an HP-5-MS fused-silica column (Ansaruzzaman, M. and Albert, M. J., unpublished data). We (0.25 mmϫ30 m) with a temperature program of 170°C f...

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Structural analysis of the lipopolysaccharide from Vibrio cholerae serotype O22

Cox

Brisson

Thibault

et al. 1997

Carbohydrate Research

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Two strains ofVibrio cholerae non-O1 possessing somatic (O) antigen factors in common withV. cholerae serogroup O139 synonym “Bengal”

Cited by 41 publications

References 15 publications

Immunochemical characterisation of Vibrio cholerae O139 O antigens and production of a diagnostic antiserum without absorption

Immunochemical characterisation of Vibrio cholerae O139 O antigens and production of a diagnostic antiserum without absorption

Structural analysis of the O‐antigenic polysaccharide from Vibrio mimicus N‐1990

Structural analysis of the lipopolysaccharide from Vibrio cholerae serotype O22

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