Two Techniques in Paper Chromatography

Disposition of Intra-Articularly Injected Hydrocortisone Acetate, Hydrocortisone and Cortisone Acetate in Arthritis. I. Concentrations in Synovial Fluid and Cells*

“…The finding of reduced blue tetrazolium at the appropriate positions on the paper chromatograms for cortisone and hydrocortisone and their acetates was considered presumptive evidence that these compounds were responsible for the reduction of the blue tetrazolium. Approximations of quantities on the chromatogram were made by the method of Tennent, Whitla and FJorey (5).…”

Steroid Formation by Adrenal Tissue from Hypertensives1

“…The strips, after drying in air, were scanned at 245 mju in a Beckman model D.U. spectrophotometer equipped with an adapter as described by Tennent, Whitla and Florey (18). The instrument was set to 100 per cent transmittance against an area of the paper strip one inch above the starting line.…”

Quantitative Determination of Buckwheat Flavonoid Substances by Direct Absorptiometry of Paper Chromatograms.

“…Many such methods have been described, all of which utilize a relation between amount of substance and some measure of zone dimension. Several devices have been used to measure the light absorption of substances on filter paper strips (3,4,14,15,16). In the present work modifications of two such techniques-those of Tennent, Whitla, and Florey (16) and of Paladini and Leloir (14)-have been applied to the estimation of rutin and four other buckwheat flavonoids.…”

“…Several devices have been used to measure the light absorption of substances on filter paper strips (3,4,14,15,16). In the present work modifications of two such techniques-those of Tennent, Whitla, and Florey (16) and of Paladini and Leloir (14)-have been applied to the estimation of rutin and four other buckwheat flavonoids. This direct absorptiometric procedure has proved adequate for the simultaneous determination of small quantities of these five substances in mixtuires.…”