Two transcription factors TaPpm1 and TaPpb1 co-regulate anthocyanin biosynthesis in purple pericarps of wheat

Jiang, Wenhui; Liu, Tianxiang; Nan, Wenzhi; Jeewani, Diddugodage Chamila; Niu, Yanlu; Li, Chunlian; Wang, Yong; Xue, Song; Wang, Cong; Wang, Jiahuan; Li, Yang; Gao, Xin; Wang, Zhonghua

doi:10.1093/jxb/ery101

Cited by 90 publications

(92 citation statements)

References 63 publications

(84 reference statements)

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“…In another study, Li et al [171] isolated an MYB transcription factor gene, TaMYB3, localised on chromosome 4BL which induces anthocyanin synthesis in the pericarp cells. Recently, Jiang et al [172] characterised two transcription factors: TaPpm1 (purple pericarp-MYB 1) and TaPpb1 (purple pericarp-bHLH 1) in wheat, and it was demonstrated that the interaction of TaPpm1 and TaPpb1 co-regulates the synthesis of anthocyanin in pericarps.…”

Section: Genetic Basis Of Purple Coloured Wheat Grainsmentioning

confidence: 99%

QTLs identified for Biofortification Traits in Wheat: A Review

Devi¹,

Kaushik²,

Saini³

2019

Preprint

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Wheat is the essential constituent of cereal-based diets and one of the most significant sources of calories. However, there is an inherently low bioavailability of proteins, mineral, and vitamins in modern wheat grains. Biofortification has earned recognition as an outstanding approach, at the same time as a cure for world hunger. The developments in the identifications of quantitative trait loci (QTL) analysis and understanding of the physiological and molecular basis of QTLs controlling the biofortification traits in wheat has revealed new horizons for the improvement of modern wheat varieties. Within this review, we have compiled the information from the studies carried out in wheat using QTL mapping methodologies that is among the best methods for biofortification traits. We hope this review will serve as an essential reference for the QTLs identified for the several important biofortification traits in wheat.that could contribute to underexploited wild relatives [25,26]. The different steps involved in the development of biofortification wheat with the aid of marker-assisted breeding are presented in Figure1. Figure 1.Representation of different strategies for biofortification of the wheat, especially explaining different steps of marker-assisted breeding.Conventional breeding methods are easy to operate with qualitative traits. These traits depend on a single gene whereas traits like yield are quantitative and therefore are impacted by several genes [27,28,29]. There are several techniques for mapping quantitative trait loci (QTL) in an experimental cross [30]. The molecular basis of QTLs is challenging to dissect, even for model plants like Arabidopsis and rice, because of the problems in precisely narrowing intervals to single genes [31]. Experimental design, type of plant population analysed and the level of polymorphisms between parental genomes also affect the predictions of QTLs. Statistical methods to determine quantitative trait loci (QTL) require numerous molecular markers with high-resolution genetic maps [32,33]. This approach is also related to genomics methods which are geared toward the dissection of complex phenotypes [34].Genomic resources of wheat have provided important support for functional genomics and conservation biology (by conserving the important landraces) [35,36]. The wheat genome is complex to interpret simply because of broadly dispersed repetitive sequences, heterozygosity, and polyploidy [37,38]. Nevertheless, the developments in sequencing methodologies, decreased sequencing price, together with the advancements in the computational resources have permitted the spread of these resources [39,40]. Besides, the comparative genomics among plant species is demonstrating to be an efficient method for the identification of novel genes regarding the biofortification of modern wheat [41]. Therefore, in this review, we have compiled the QTLs Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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Section: Genetic Basis Of Purple Coloured Wheat Grainsmentioning

confidence: 99%

QTLs identified for Biofortification Traits in Wheat: A Review

Devi¹,

Kaushik²,

Saini³

2019

Preprint

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“…Pp3 (mapped to chromosome 2A) encodes a bHLH transcription factor (Shoeva et al, 2014). The Pp-1 genes (7A, 7B, 7D) encode R2R3-MYB-type transcription factors (Jiang et al, 2018). The Ba genes responsible for blue aleurone were transferred into cultivated wheat varieties from distant relatives such as Thinopyrum ponticum, Th.…”

Section: Regulation Of Anthocyanin Biosynthesis In Wheat Pericarpmentioning

confidence: 99%

Anthocyanins in wheat grain: genetic control, health benefit and bread-making quality

2019

Plant Genetic Resources for Breeding and Producing Functional Nutraceutical Food

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Anthocyanins are plant secondary metabolites having multiple biological functions. In this paper, we summarize data on anthocyanins in wheat grain, including molecular-genetic mechanisms underlying the synthesis of anthocyanins in the grain pericarp, the effect of anthocyanins on bread-making quality and health benefit. Anthocyanin synthesis in wheat pericarp tissue is controlled by two complementary genes, Pp3/TaMyc1 and Pp-1, mapped to chromosome 2A and homologous group 7 chromosomes, respectively. These genes encode transcription factors with the bHLH and R2R3-MYB-regulatory domains, respectively, which activate expression of the anthocyanin biosynthesis structural genes, encoding enzymes of the pathway, in a tissue-specific manner. A comprehensive study of the effect of wheat genes associated with the level of anthocyanins in grain on the preventive effect of grain products in neurodegenerative disorders was carried out. The use of near-isogenic lines (NILs) having almost similar genomes with the exception of a small part of chromosome 2A, which contains Pp3, has allowed relating the observed differences precisely with anthocyanin biosynthesis. As a result, it was revealed that the Pp3 gene is related to the production of dietary products preventing neurodegenerative changes caused by Alzheimer's and Parkinson's diseases and natural aging. The beneficial effect of a grain diet with a high content of anthocyanins on the working memory and molecular processes of neuroregeneration was shown on mice. The use of the same wheat NILs for testing bread-making quality showed the resistance of anthocyanins to the backing process and a better quality and organoleptic properties of bakery products made from anthocyanin-rich grains in comparison with products obtained from control grains. An optimal scheme for the accelerated creation of wheat breeding lines with a high content of anthocyanins in the grain pericarp is considered.

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“…Based on the high-throughput screening and stringent screening pressure, many researches have obtained candidate prey proteins by yeast one-and two-hybrid assays, like in wheat [3,4], Arabidopsis [5], rice [6], populus [7], kiwifruit [8] and so on. Moreover, increasing numbers of studies focused on improving the screening efficiency, saving time and decreasing the cost required to identify target molecules in yeast one-and two-hybrid system assays [9,10].…”

Section: Introductionmentioning

confidence: 99%

Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assays

Zhou

Liu

et al. 2020

BMC Biotechnol

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Background: Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate (MeJA) signaling pathway, one of the main components of flower fragrance in C. faberi, yeast one-and two-hybrid three-frame cDNA libraries were constructed. Results: In this study, a modified cDNA library used for yeast one-and two-hybrid screening was successfully constructed, with a recombinant efficiency of 95%. The lengths of inserted fragments ranged from 750~3000 bp, and the library capacity reached 6 × 10 9 CFU/ μg of cDNA insert, which was suitable for the requirements of subsequent screening. Finally, a homologous protein related with pathogenesis was screened out by the bait vector of CfbHLH36, which may participate in the MeJA signaling pathway.Conclusion: The yeast one-and two-hybrid library of C. faberi provides large amounts of useful information for the functional genomics research in C. faberi, and this method could also be applied to other plants to screen DNAprotein and protein-protein interactions.

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Two transcription factors TaPpm1 and TaPpb1 co-regulate anthocyanin biosynthesis in purple pericarps of wheat

Abstract: Two genes controlling the purple pericarp trait in wheat, TaPpm1 and TaPpb1, are identified and the mechanism by which they co-regulate anthocyanin synthesis is proposed.

Cited by 90 publications

References 63 publications

QTLs identified for Biofortification Traits in Wheat: A Review

QTLs identified for Biofortification Traits in Wheat: A Review

Anthocyanins in wheat grain: genetic control, health benefit and bread-making quality

Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assays

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