Two transcripts, differing at their 3′ ends, are produced from the Candida albicans SEC14 gene

Riggle, Perry J.; Slobodkin, I. V.; Brown, D. H.; Hanson, Mark P.; Volkert, Thomas L.; Kumamoto, Carol A.

doi:10.1099/00221287-143-11-3527

Cited by 14 publications

(9 citation statements)

References 27 publications

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“…Deletion of one allele conferred no observable phenotype, but attempts to delete the second allele were without success, suggesting that SEC14 is essential 80 . Similar results were obtained in an independent study of SEC14 81 and conditional expression of SEC14 using the GRACE method also indicates that it is essential 35 …”

Section: Golgi To the Plasma Membrane Transport And Exocytosissupporting

confidence: 71%

The protein secretory pathway of Candida albicans

Fonzi

2009

Mycoses

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Virulence of the opportunistic pathogen, Candida albicans, relies on an assemblage of attributes. These include the secretion of hydrolytic enzymes, cell surface adhesins, morphological transition between yeast and hyphae, phenotypic switching and biofilm formation. These diverse features are united by their dependence on the protein secretory apparatus for expression. Although the secretory apparatus of C. albicans has been studied limitedly, it appears to conform to the well-conserved eukaryotic system of vesicle-mediated transport between intracellular compartments and the cell surface. Genome comparison with Saccharomyces cerevisiae, however, shows multiple differences whose functional significance is yet unstudied. A unique aspect of the secretory pathway of C. albicans is its structural, and perhaps functional, rearrangement in hyphal vs. yeast cells. This, and evidence of non-conserved secretion mechanism(s), suggest that there is much fundamental knowledge to be derived from the analysis of secretion in C. albicans, which will be relevant to its ability to cause disease.

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Section: Golgi To the Plasma Membrane Transport And Exocytosissupporting

confidence: 71%

The protein secretory pathway of Candida albicans

Fonzi

2009

Mycoses

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“…2). Changes in levels of alternatively spliced transcripts also occur between the long and short C. albicans SEC14 transcripts during budding and filamentous growth (Riggle et al 1997). This regulation at the transcript level may then lead to changes in expressed isoform activities, stabilities, and subcellular localizations.…”

Section: Resultsmentioning

confidence: 97%

Genomic characterization of human SEC14L1 splice variants within a 17q25 candidate tumor suppressor gene region and identification of an unrelated embedded expressed sequence tag

et al. 2001

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Human SEC14L1 shows partial sequence homology to the budding yeast SEC14 protein and the Japanese flying squid retinal-binding protein and was previously generally localized to 17q25. We more precisely mapped SEC14L1 within a discrete region of 17q25 that likely harbors at least one putative breast and ovarian tumor suppressor gene. We determined that this gene consists of 18 exons ranging in size from 70 bp (exon 11) to 3088 bp (exon 17) and spanning at least 58 kb of DNA. Exon 17 contained a highly polymorphic variable number of tandem repeats (VNTR) and was present only in the larger ubiquitously expressed 5.5-kb transcript. The 3.0-kb ubiquitously expressed transcript included sequences at the beginning of exon 17 (designated exon 17a) and the end of exon 17 (designated exon 18), but lacked the internal 2439 bp of exon 17, including the VNTR. This alternative splicing resulted in a predicted protein of 719 residues from the smaller transcript with four more terminal amino acids than the 715 residue protein predicted from the larger transcript. EST H49244 spanned exon 11 of SEC14L1 and was specifically expressed in human peripheral blood leukocytes. One intragenic single nucleotide polymorphism (SNP) was confirmed. SEC14L1 contained the CRAL/TRIO domain also found in alpha-tocopherol transfer protein (TTPA) and cellular retinaldehyde-binding protein (CRALBP). As retinoids have been shown to inhibit the growth of breast cancer cells, loss of the proposed SEC14L1 retinal-binding function may contribute to breast tumorigenesis. As TTPA and CRALBP have been implicated in retinitis pigmentosa (RP), altered SEC14L1 expression may contribute to RP in previously unlinked families. Coding exon-specific PCR primers were designed to aid in future expression and mutational analyses.

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“…This suppressor analysis suggests a role for PLD1 in secretion, particularly in the budding of nascent secretory vesicles from the transGolgi network. Furthermore, work on Sec14 in both Yarrowia lipolytica (Lopez et al, 1994) and C. albicans (Monteoliva et al, 1996 ;Riggle et al, 1997) has revealed a possible role for Sec14 in morphogenesis. Sec14 has been proposed to regulate the DAG pool necessary for proper secretory function (Kearns et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

The role and relevance of phospholipase D1 during growth and dimorphism of Candida albicans

et al. 2001

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The phosphatidylcholine-specific phospholipase D1 (PLD1) in Saccharomyces cerevisiae is involved in vesicle transport and is essential for sporulation. The gene encoding the homologous phospholipase D1 from Candida albicans (PLD1) was used to study the role of PLD1 in this pathogenic fungus. In vitro and in vivo expression studies using Northern blots and reverse transcriptase-PCR showed low PLD1 mRNA levels in defined media supporting yeast growth and during experimental infection, while enhanced levels of PLD1 transcripts were detected during the yeast to hyphal transition. To study the relevance of PLD1 during yeast and hyphal growth, an essential part of the gene was deleted in both alleles of two isogenic strains. In vitro PLD1 activity assays showed that pld1 mutants produced no detectable levels of phosphatidic acid, the hydrolytic product of PLD1 activity, and strongly reduced levels of diacylglycerol, the product of lipid phosphate phosphohydrolase, suggesting no or a negligible background PLD1 activity in the pld1 mutants. The pld1 mutants showed no growth differences compared to the parental wild-type in liquid complex and minimal media, independent of the growth temperature. In addition, growth rates of pld1 mutants in media with protein as the sole source of nitrogen were similar to growth rates of the wild-type, indicating that secretion of proteinases was not reduced. Chlamydospore formation was normal in pld1 mutants. When germ tube formation was induced in liquid media, pld1 mutants showed similar rates of yeast to hyphal transition compared to the wild-type. However, no hyphae formation was observed on solid Spider medium, and cell growth on cornmeal/Tween 80 medium indicated aberrant morphogenesis. In addition, pld1 mutants growing on solid media had an attenuated ability to invade the agar. In a model of oral candidosis, pld1 mutants showed no attenuation of virulence. In contrast, the mutant was less virulent in two different mouse models. These data suggest that PLD1 is not essential for growth and oral infections. However, they also suggest that a prominent part of the phosphatidic acid and diacylglycerol pools is produced by PLD1 and that the level of these components is important for morphological transitions under certain conditions in C. albicans.

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Two transcripts, differing at their 3′ ends, are produced from the Candida albicans SEC14 gene

Cited by 14 publications

References 27 publications

The protein secretory pathway of Candida albicans

The protein secretory pathway of Candida albicans

Genomic characterization of human SEC14L1 splice variants within a 17q25 candidate tumor suppressor gene region and identification of an unrelated embedded expressed sequence tag

The role and relevance of phospholipase D1 during growth and dimorphism of Candida albicans

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