Two types of coumarins-specific enzymes complete the last missing steps in pyran- and furanocoumarins biosynthesis

The telomere-to-telomere (T2T) genome of Peucedanum praeruptorum Dunn provides insights into the genome evolution and coumarin biosynthesis

“…PpPT1 (ON934685) has a umbelliferone 6-prenyltransferase (U6P) activity, and PpPT2 (ON934686) has a umbelliferone 8-prenyltransferase (U8P) activity with a minor U6P activity. PpPT3 (ON934687) has both U6P and U8P activities, whereas the rest of the 3 homologs have weak U6P activities [ 9 ]. Additionally, 2 CYP P450 monooxygenases, PpDC (ON934691) and PpOC (ON934692), have been identified [ 9 ].…”

“…PpPT3 (ON934687) has both U6P and U8P activities, whereas the rest of the 3 homologs have weak U6P activities [ 9 ]. Additionally, 2 CYP P450 monooxygenases, PpDC (ON934691) and PpOC (ON934692), have been identified [ 9 ].…”

“…Currently, through the analysis of both transcriptomic and metabolomic data, 3 distinct prenyltransferases (PpPT1–3) in P. praeruptorum have been identified as responsible for the prenylation of the simple coumarin skeleton, forming linear or angular precursors. Additionally, 2 novel CYP450 cyclases (PpDC and PpOC) have been shown to be responsible for the cyclization of these linear/angular precursors into either tetrahydrofurans or tetrahydropyrans [ 9 ]. Another recent study in P. praeruptorum also combined comparative transcriptomics and metabolomics to provide insights into transcriptional changes and the reduction of coumarins after blooming, disclosing the key gene regulatory networks of coumarin biosynthesis in P. praeruptorum at the vegetative growth stages and reproductive stages [ 10 ].…”

Research progress on the biosynthesis of plant phenylpropanoids

“…七叶亭或东莨菪内酯 [63,64] 。2019 年 Vanholme 等人从 拟南芥中筛选到一个香豆素合酶 (coumarin synthase， COSY)可以催化 2,4-二羟基肉桂酰辅酶 A 等生成伞 形酮等简单香豆素类化合物， 对该类化合物的生物合 成途径进行了完善 [65] (图 5) 。 伞形酮通过异戊烯基不同位置的取代形成线型 或角型香豆素前体。2021 年 Villard 等人从桑科无花 果树中鉴定了一个氧化酶 CYP76F112，以 6 位异戊 烯基化修饰的伞形酮(去甲基软木花椒素)为底物催 化合成线型呋喃香豆素异紫花前胡内酯 [66] (图 5) 。 2023 年中国药科大学孔令义教授团队利用代谢组-转 录组联合分析、系统进化分析、共表达分析等策略从 白花前胡中挖掘并表征了三条异戊烯基转移酶及两 种新型环化酶，阐明了线型和角型呋喃/吡喃香豆素 基本母核的生物合成机制 [67] 李凯薇等: 植物苯丙素类天然产物生物合成研究进展 umbelliferone 6-prenyltransferase (U6PT); PpPT2：umbelliferone 8-prenyltransferase (U8PT); PpPT3：umbelliferone 6/8-prenyltransferase (U6,8PT); PpDC：demelthyisuberosin cyclase; PpOC：osthenol cyclase; CmOMT1/2：O-methyltransferase 1/2 from Cnidium monnieri; R1-R4 represent different substituent groups.…”