Two types of jaw-muscle spindle afferents in the cat as demonstrated by intra-axonal staining with HRP

Shigenaga, Yoshio; Mitsuhiro, Y.; Shirana, Y.; Tsuru, Hiromichi

doi:10.1016/0006-8993(90)91418-g

Cited by 61 publications

(71 citation statements)

References 35 publications

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“…Additional evidence that the stained afferents are jaw-muscle spindle afferents comes from the fact that the biotinamide-stained somata of all intracellularly stained neurons were located within the trigeminal mesencephalic nucleus, and all exhibited the pseudounipolar soma morphology characteristic of such neurons. Finally, the distribution and size of axon collaterals and boutons displayed by the neurons intracel- lularly stained in this study are comparable to what has been previously described for jaw-muscle spindle afferents (Shigenaga et al, 1988a(Shigenaga et al, , 1990Dessem and Taylor, 1989;Ligenhöhl and Friauf, 1991;Dessem et al, 1997).…”

Section: Jaw-muscle Spindle Afferentssupporting

confidence: 78%

“…Although jaw-muscle spindle afferent input to neurons in the supratrigeminal region has previously been reported (Miyazaki and Luschei, 1987;Shigenaga et al, 1988aShigenaga et al, , 1990Yabuta et al, 1996;Dessem et al, 1997;Kishimoto et al, 1998;Yoshida et al, 1999), it has not been shown that Vsup neurons which receive muscle spindle afferent input project to the trigeminal motor nucleus. Due to the close proximity of the Vsup to the HRP injection site, we were unable to clearly distinguish between retrogradely labeled, ipsilateral Vsup neurons and neurons which may have incorporated horseradish peroxidase directly from the injection site.…”

Section: Premotor Neurons Receiving Jaw-muscle Spindle Afferent Inputmentioning

confidence: 96%

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Jaw‐muscle spindle afferent pathways to the trigeminal motor nucleus in the rat

Luo

Moritani

Dessem

2001

J of Comparative Neurology

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Neural pathways conveying proprioceptive feedback from the jaw muscles were studied in rats by combining retrograde and intracellular neuronal labeling. Initially, horseradish peroxidase was iontophoresed unilaterally into the trigeminal motor nucleus (Vmo). Two days later, 1-5 jaw-muscle spindle afferent axons located in the mesencephalic trigeminal nucleus were physiologically identified and intracellularly stained with biotinamide. Stained mesencephalic trigeminal jaw-muscle spindle afferent axon collaterals and boutons were predominantly distributed in the supratrigeminal region (Vsup), Vmo, dorsomedial trigeminal principal sensory nucleus (Vpdm), parvicellular reticular formation (PCRt), alpha division of the parvicellular reticular formation (PCRtA), and dorsomedial portions of the spinal trigeminal subnuclei oralis (Vodm), and interpolaris (Vidm). Numerous neurons retrogradely labeled with horseradish peroxidase from the trigeminal motor nucleus were found bilaterally in the PCRt, PCRtA, Vodm, and Vidm. Retrogradely labeled neurons were also present contralaterally in the Vsup, Vpdm, Vmo, peritrigeminal zone, and bilaterally in the dorsal medullary reticular field. Putative contacts between intracellularly stained mesencephalic trigeminal jaw-muscle spindle afferent boutons and trigeminal premotor neurons retrogradely labeled with horseradish peroxidase were found in the ipsilateral Vodm, PCRtA, and PCRt, as well as the contralateral Vsup, Vmo, Vodm, PCRt, and PCRtA. Thus, multiple disynaptic jaw-muscle spindle afferent-motoneuron circuits exist. These pathways are likely to convey long-latency jaw-muscle stretch reflexes and may contribute to stiffness regulation of the masticatory muscles.

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Section: Jaw-muscle Spindle Afferentssupporting

confidence: 78%

Section: Premotor Neurons Receiving Jaw-muscle Spindle Afferent Inputmentioning

confidence: 96%

Jaw‐muscle spindle afferent pathways to the trigeminal motor nucleus in the rat

Luo

Moritani

Dessem

2001

J of Comparative Neurology

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“…On the other hand, all of the descending axons traveled within Vjux, PR.f, or PGd, with their path gradually directed medially. This axonal trajectory is quite similar to that of the Probst's tract (Gottlieb et al, 1984;Nomura and Mizuno, 1985;Shigenaga et al, 1988aShigenaga et al, ,b, 1989aShigenaga et al, , 1990a and passes through regions where neurons projecting to Vmo were observed (Mizuno et al, 1983;Travers and Norgren, 1983;Landgren et al, 1986). The neurons responsible for connecting trigeminal sensory (Panneton and Burton, 1982;Ikeda et al, 1984;Nasution and Shigenaga, 1987).…”

Section: Definition Of Vo Neuronsmentioning

confidence: 63%

Two major types of premotoneurons in the feline trigeminal nucleus oralis as demonstrated by intracellular staining with horseradish peroxidase

Yoshida

Yasuda

Dostrovsky³

et al. 1994

J of Comparative Neurology

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Previous studies suggest that neurons in the dorsomedial subdivisions of trigeminal nucleus oralis (Vo) may contribute to reflex control of jaw movements and to modulation of sensory information. The present study has addressed this possibility by the use of intracellular staining with horseradish peroxidase of physiologically identified neurons in Vo to examine functional and morphological properties of these neurons. Of 14 labeled neurons, eight had axon collaterals terminating exclusively in the dorsolateral subdivision of the trigeminal motor nucleus (DL neurons) and four in its ventromedial subdivision (VM neurons); axon collaterals of two neurons were not traced. Both groups of neurons sent terminal arbors into other nuclei of the lower brainstem. The DL neurons were distinguishable from the VM neurons in their receptive field (RF) location, neuronal position, somadendritic architecture, and projections to other brainstem nuclei. All neurons, except for two that were exclusively activated by noxious stimuli applied to the tongue, were responsive to light mechanical stimulation of peri- and intraoral structures. The RFs of the DL neurons were located in more posterior oral structures than those of the VM neurons. The RF of nearly all low-threshold DL neurons was located in the maxillary region, and that of the VM neurons, in contrast, involved the mandibular region. The VM neurons were located medial or ventral to the DL neurons. The soma size of the VM neurons was significantly larger than that of the DL neurons. Dendritic arbors of both groups could be separated into medial and lateral components. The ratio of the dendritic transverse areas in the medial vs. lateral component was significantly higher in the VM neurons than in the DL neurons. The DL neurons also issued collaterals that terminated in larger brainstem areas than those of the VM neurons. These observations provide new evidence on the morphological and functional properties of Vo neurons that contribute to reflex control of jaw and facial movements and modulation of sensory information.

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“…In a previous study we labeled 13 jaw muscle spindle afferents by intraaxonal injection of HRP and found that two of these afferents gave off axon collaterals that terminated in the Vo.r (Shigenaga et al, 1990a). In a comparable study, Luo et al (1995) also found a projection of jaw muscle spindle afferents to the Vo.r.…”

Section: Premotor Neurons In the Vormentioning

confidence: 78%

Quantitative ultrastructure of physiologically identified premotoneuron terminals in the trigeminal motor nucleus in the cat

et al. 2000

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Little is known about the ultrastructure of synaptic boutons contacting trigeminal motoneurons. To address this issue, physiologically identified premotor neurons (n = 5) in the rostrodorsomedial part of the oral nucleus (Vo.r) were labeled by intracellular injections of horseradish peroxidase (HRP) in cats. The ultrastructure of 182 serially sectioned axon terminals from the five neurons was both qualitatively and quantitatively analyzed. In addition, the effects of the glycine antagonist strychnine, GABA(A) antagonist bicuculline, NMDA antagonist 2-amino-5-phosphonovalerate (APV), and non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) on Vo.r-induced postsynaptic potentials in trigeminal motoneurons (n = 11) were examined to evaluate potential signaling substances of the premotor neurons. Labeled boutons made synaptic contacts with either jaw-closing or -opening motoneurons. All the boutons contained pleomorphic vesicles, and most formed a single symmetric synapse either on the somata or on primary dendrites. Morphometric analyses indicated that bouton volume, bouton surface area, apposed surface area, total active zone area, and mitochondrial volume were not different between boutons on jaw-closing and -opening motoneurons. Vesicle number and density, however, were higher for boutons on jaw-closing motoneurons. The five morphological parameters were positively correlated with bouton volume. Vesicle density was the exception, which tending to be negatively correlated. Intravenous infusion of strychnine or bicuculline suppressed Vo.r-induced inhibitory postsynaptic potentials (IPSPs) in jaw-closing motoneurons. Abolition of Vo. r-induced excitatory postsynaptic potentials in jaw-opening motoneurons with APV and CNQX unmasked IPSPs. The present results suggest that premotor neurons in the Vo.r are inhibitory and that positive correlations between the ultrastructural parameters associated with synaptic release and bouton size are applicable to the interneurons, as they are in primary afferents.

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Two types of jaw-muscle spindle afferents in the cat as demonstrated by intra-axonal staining with HRP

Cited by 61 publications

References 35 publications

Jaw‐muscle spindle afferent pathways to the trigeminal motor nucleus in the rat

Jaw‐muscle spindle afferent pathways to the trigeminal motor nucleus in the rat

Two major types of premotoneurons in the feline trigeminal nucleus oralis as demonstrated by intracellular staining with horseradish peroxidase

Quantitative ultrastructure of physiologically identified premotoneuron terminals in the trigeminal motor nucleus in the cat

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