Two Viruses from the Lucke Tumor Isolated in a Frog Pronephric Cell Line

Wong, W. Y.; Tweedell, Kenyon S.

doi:10.3181/00379727-145-37981

Cited by 20 publications

(14 citation statements)

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“…In a cell line derived from a carcass of metamorphosing X. laevis, the cell count increased 7 or 8 times in 5 days (Pudney et al., 1973). Other population doubling times reported were 46 hr for cells derived from R. pipiens kidneys (Malamud, 1967), 17 45 hr for cells from X. laevis kidneys (Godsell, 1974), 90 hr for cells from R. pipiens pronephroi (Wong and Tweedell, 1974), 39 hr for cells from X. laevis tadpoles (Anizet et al, 1981), 68 hr at 25°C for cells from Bombina orientalis embryos (Ellinger et al, 1983), and 75.5 hr at 25°C for cells from a tumor of X. laevis (Fukui et al, 1992).…”

Section: Population Doubling Timementioning

confidence: 99%

“…In amphibian cell cultures, there are reports of cells with normal chromosomal constitutions and those with aberrant ones (Wolf and Quimby, 1964;Rafferty, 1969;Freed and Mezger-Freed, 1970;Pudney et al, 1973;Wong and Tweedell, 1974;Handler et al, 1979;Anizet et al, 1981;Ellinger et al, 1983;Kondo and Ide, 1983;Fukui et al, 1992).…”

Section: Chromosomal Constitutionmentioning

confidence: 99%

“…A considerable number of studies have been reported hitherto on the isolation and cultivation of amphibian cells from embryos, tadpoles, and adults of various species, such as Rana pipiens (Auclair, 1961;Malamud, 1967;Rafferty, 1969;Freed et al, 1969;Freed and Mezger-Freed, 1970;Wong and Tweedell, 1974), Rana sylvatica, Rana clamitans, Hyla crucifer (Rafferty, 1969), Rana catesbeiana (Wolf and Quimby, 1964;Ide,1973Ide, ,1974Kondo and Ide, 1983;Yosizato, 1985, 1986;Yoshizato and Nishikawa, 1985), Bufo marinus (Handler et al, 1979), Bufo americanus (Freed et al, 1969), Bombina orientalis (Ellinger et al, 1983), Xenopus laevis (Pudney et al, 1973;Godsell, 1974;Miller and Daniel, 1977;Anizet et al, 1981;Asashima et al, 1986;Nishikawa et al, 1990;Fukui et al, 1992) and Notophthalmus viridescens (Ferretti and Brockes, 1988). However, most of these studies were concerned with relatively short-term cultures.…”

Section: Introductionmentioning

confidence: 99%

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Establishment of Three Cell Lines Derived from Frog Melanophores

Okumoto

2001

Zoological Science

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Section: Population Doubling Timementioning

confidence: 99%

Section: Chromosomal Constitutionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Establishment of Three Cell Lines Derived from Frog Melanophores

Okumoto

2001

Zoological Science

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“…Another herpesvirus-transformed pronephric cell line. WMPa/RT 217, previously infected with LTHV [53] was also utilized. Although virus replication had ceased, viral antigens were still detectable in the trans formed cells by LTHV antiserum [49].…”

Section: Cell Origin and Tissue Culturementioning

confidence: 99%

“…The carcinomas were developed after injection of Lucke tumor herpesvirus-enriched fractions of renal tumors into the nephrogenic ridge of R. pipiens embryos [46]. A normal pronephric cell line, WMPa, was used for comparison [53]. Another herpesvirus-transformed pronephric cell line.…”

Section: Cell Origin and Tissue Culturementioning

confidence: 99%

Changes in Nucleolar and Ribosomal RNA of the Frog Kidney after Malignant Transformation by the Lucke Tumor Herpesvirus

Tweedell¹,

Malin²

1984

Pathobiology

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Malignant transformation of the frog kidney by the Lucke herpesvirus changes the nucleotide base composition of normal kidney nucleolar and ribosomal RNA. In the Lucke tumor there is a moderate decline in guanylic acid and a sharp decline in adenylic acid levels. Conversely, there is a sharp increase in cytidylic acid and uridylic acid levels in the tumor cells. However, there was an increase in the G + C content of nucleolar and ribosomal RNA over that obtained from the normal kidney cells. Nearly identical quantitative changes in the base composition of each RNA species were measured for the adult (spontaneous) mesonephric carcinoma and a Lucke-herpesvirus-induced pronephric tumor cell line; similar correspondence was obtained for the normal adult mesonephros and a normal pronephric cell line.

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Viruses and renal carcinoma of rana pipiens. XV. The presence of virus‐associated membrane antigen(s) on lucké tumor cells

Naegele

Granoff

1977

Intl Journal of Cancer

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Virus-specific antigens were detected in Lucké tumor cells by indirect immunofluorescence using antiserum prepared against Lucké herpesvirus. Intracellular fluorescence, both cytoplasmic and nuclear, was observed only in acetone:methanol-fixed tumor cells that contained herpesvirus detected by electron microscopy. The number of positive cells correlated well with the number of cells containing virus. In contrast, both virus-containing and virus-free cells exhibited membrane fluorescence when viable unfixed tumor cells were tested. A striking reduction in the number of membrane fluorescent cells was observed with an increase in the length of time that tumor cells were in primary culture. No reaction was observed with a variety of normal R. pipiens cells. Absorption of the antiserum with normal frog kidney tissue had no effect on the number of positive cells whereas absorption with virus-free tumor reduced the reaction; absorption with virus-containing tumor eliminated it. These findings provide the first demonstration that the Lucké herpesvirus genome resident in virus-free tumor cells expresses a virus-associated membrane antigen(s).

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Two Viruses from the Lucke Tumor Isolated in a Frog Pronephric Cell Line

Cited by 20 publications

References 0 publications

Establishment of Three Cell Lines Derived from Frog Melanophores

Establishment of Three Cell Lines Derived from Frog Melanophores

Changes in Nucleolar and Ribosomal RNA of the Frog Kidney after Malignant Transformation by the Lucke Tumor Herpesvirus

Viruses and renal carcinoma of rana pipiens. XV. The presence of virus‐associated membrane antigen(s) on lucké tumor cells

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